EP0136907A2
Expired / Lapsed
EP
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A xenogeneic expression control system, a method of using it, expression vectors containing it, cells transformed thereby and heterologous proteins produced therefrom
Assignee: Genentech Inc
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Filing: 1984-10-01
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Granted: —
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Expires: 2004-10-01
Abstract
Abstract A portable supplementary control system comprising a promoter/operator operably linked to a gene encoding the desired heterologous protein plus a gene encoding a compatible supplementary repressor protein operably linked to an inducible promotor, thus remedying any deficiency of repressor provided by the host's own genome, so that expression of a gene encoding a heterologous protein is repressed until a favourable point in the growth cycle of the host. The control system offers a method whereby heterologous protein production can be regulated by manipulation of the environment or of the medium.
Inventors
- 👤 Herbert Louis Heyneker
- 👤 Norm Shin Chsiang Lin
- 👤 Donna Thourson Palmer
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 21:44
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/635; C12N15/70; C12N15/72
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
80 words
A control system useful in suppressing the expression of a gene encoding a protein heterologous to a prokaryotic host which system comprises: a first DNA sequence which comprises a promoter/operator operably linked to the gene encoding said protein; and a second DNA sequence which comprises an inducible promoter operably linked to the gene encoding a repressor protein compatible with the promoter/operator, and supplementary to the host; wherein the first DNA sequence and second DNA sequence are xenogeneic to the host.
Claim 2
Dependent Claim
80 words
A control system useful in suppressing the expression of a gene encoding a protein heterologous to a prokaryotic host which system comprises: a first DNA sequence which comprises a promoter/operator operably linked to the gene encoding said protein; and a second DNA sequence which comprises an inducible promoter operably linked to the gene encoding a repressor protein compatible with the promoter/operator, and supplementary to the host; wherein the first DNA sequence and second DNA sequence are xenogeneic to the host.
Claim 3
Dependent Claim
15 words
The control system of Claim 1 wherein the first DNA sequence comprises the trp promoter/operator.
Claim 4
Dependent Claim
15 words
The control system of Claim 1 wherein the second DNA sequence comprises the lac promoter.
Claim 5
Dependent Claim
21 words
The control system of Claim 2 wherein the second DNA sequence comprises the lac promoter operably linked to the trpR gene.
Claim 6
Dependent Claim
15 words
The control system of Claim 1 wherein the host is a strain of E. coli.
Claim 7
Dependent Claim
34 words
The control system of Claim 1 wherein the heterologous protein is selected from the group consisting of FMD antigenic protein, a mammalian growth hormone, a mammalian interferon, human tissue plasminogen activator, urokinase and IL-2.
Claim 8
Dependent Claim
28 words
An expression vector effective in prokaryotic host cells in expressing a gene encoding a desired protein heterologous to said host, which comprises the control system of Claim 1.
Claim 9
Dependent Claim
12 words
Cells or a cell culture transformed with the vector of Claim 7.
Claim 10
Dependent Claim
19 words
A protein produced by expression of the gene encoding a heterologous protein disposed in the vector of Claim 7.
Claim 11
Dependent Claim
22 words
A method of controlling the expression of a gene encoding a heterologous protein, which method comprises culturing the cells of Claim 8.
Claim 12
Dependent Claim
90 words
-11. A method of controlling the expression of a gene encoding a desired heterologous protein, which method comprises culturing cells transformed with an expression vector for said gene which vector comprises a first DNA sequence which comprises a promoter/ operator operably linked to the gene encoding said protein; and a second DNA sequence which comprises an inducible promoter operably linked to the gene encoding a repressor protein compatible with the promoter/operator, and supplementary to the host; wherein the first DNA sequence and second DNA sequence are xenogeneic to the host.
Claim 13
Dependent Claim
90 words
-11. A method of controlling the expression of a gene encoding a desired heterologous protein, which method comprises culturing cells transformed with an expression vector for said gene which vector comprises a first DNA sequence which comprises a promoter/ operator operably linked to the gene encoding said protein; and a second DNA sequence which comprises an inducible promoter operably linked to the gene encoding a repressor protein compatible with the promoter/operator, and supplementary to the host; wherein the first DNA sequence and second DNA sequence are xenogeneic to the host.
References Cited (Backward Citations)
No citation records indexed.
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