EP0136362A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Biosensor
Assignee: Panasonic Holdings Corp
•
Filing: 1984-03-06
•
Granted: —
•
Expires: 2004-03-06
Abstract
Abstract A measuring electrode (27) and an adjacent electrode (28) are embedded in an insulating substrate (26). The exposed surface of the electrode system is coated with a porous substance (29) carrying an oxidation reductase (30) and, if needed, an electron acceptor (31) in a dry state, to form a biosensor. A predetermined voltage is applied between the electrodes (27, 28) of the biosensor, and a matrix-containing sample is dropped on the porous substance (29). A current proportional to the substrate concentration flows, so it is possible to quantitatively determine the matrix concentration of various biological samples highly accurately, rapidly and easily.
Inventors
- 👤 Mariko Kawaguri
- 👤 Shiro Nankai
- 👤 Takashi Iijima
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-29 14:45
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; C12Q1/004
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
99 words
In a bio-sensor for measuring the substrate concentration of a liquid sample by putting an electrode system comprising at least a working electrode and a counter electrode on an insulating substrate plate, bringing an enzyme, electron acceptor and said liquid sample to reaction, and detecting an electrochemical change caused by said reaction on said electrode system, a bio-sensor characterized in that the substrate concentration of said liquid sample is measured by covering the exposed surface of said electrode system with a porous substrate containing oxidoreductase and electrochemically detecting the electron acceptor reduced by said reaction on said electrode system.
Claim 2
Dependent Claim
99 words
In a bio-sensor for measuring the substrate concentration of a liquid sample by putting an electrode system comprising at least a working electrode and a counter electrode on an insulating substrate plate, bringing an enzyme, electron acceptor and said liquid sample to reaction, and detecting an electrochemical change caused by said reaction on said electrode system, a bio-sensor characterized in that the substrate concentration of said liquid sample is measured by covering the exposed surface of said electrode system with a porous substrate containing oxidoreductase and electrochemically detecting the electron acceptor reduced by said reaction on said electrode system.
Claim 3
Dependent Claim
19 words
A bio-sensor as described in Claim 1 characterized in that said porous substrate carried said oxidoreductase and electron acceptor.
Claim 4
Dependent Claim
24 words
A bio-sensor as described in Claim 1 characterized in that said working electrode comprises platinum and said counter electrode comprises platinum or silver/silver chloride.
Claim 5
Dependent Claim
26 words
A bio-sensor as described in Claim 1 characterized in that said electrode system is composed of a working electrode, a counter electrode and a reference electrode.
Claim 6
Dependent Claim
18 words
A bio-sensor as described in Claim 1 characterized in that said porous substrate is a hydrophilic porous membrane.
Claim 7
Dependent Claim
15 words
A bio-sensor as described in Claim 1 characterized in that said electron acceptor is oxygen.
Claim 8
Dependent Claim
30 words
A bio-sensor as described in claim 1 characterized in that said enzyme is one member selected from the group consisting of glucose oxidase, alcohol oxidase, xanthine oxidase and cholesterol oxidase.
Claim 9
Dependent Claim
25 words
A bio-sensor as described in Claim 1 characterized in that, as said electron acceptor, an oxidizing dye working in conjugation with said oxidoreductase is used.
Claim 10
Dependent Claim
39 words
A bio-sensor as described in Claim 8, wherein, as said oxidizing dye working in conjugation with the oxidoreductase, one member selected from the group consisting of potassium ferricyanide, p-benzoquinone, 2,6-dichlorophenolindophenol, methylene blue, phenazine methosulfate and potassium B-naphtoquinone-4-sulfonate is used.
Claim 11
Dependent Claim
22 words
A bio-sensor as described in Claim 8 characterized in that said oxidoreductase is glucose oxidase and said electron acceptor is potassium ferrocyanide.
Claim 12
Dependent Claim
24 words
A bio-sensor as described in Claim 5 characterized in that at least the oxidoreductase in a dry state is carried on said porous membrane.
Claim 13
Dependent Claim
21 words
A bio-sensor as described in Claim 5, wherein said porous membrane has a pore diameter of not more than 3 pm.
Claim 14
Dependent Claim
23 words
A bio-sensor as described in Claim 5 characterized in that said porous substrate is a one previously treated with a surface active agent.
Claim 15
Dependent Claim
18 words
A bio-sensor as described in Claim 5 characterized in that an anticoagulant is carried on said porous membrane.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.