EP0133035A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Process for the preparation of cellulase
Assignee: Research Association for Petroleum Alternatives Development
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Filing: 1984-07-27
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Granted: —
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Expires: 2004-07-27
Abstract
Abstract Cellulase may be prepared in good yield and at relatively low cost by culturing certain mutant strains of the genus Trichoderma, which exhibit increased inducibility of cellulase by L-sorbose, in medium wherein the carbon source comprises cellulose-containing material of plant origin, for example, bagasses, waste papers, rice plant hulls and straws or soybean wastes. Preferred mutant strains are exemplified by Trichoderma reesei PC-1-4, PC-3-7, X-30 and X-31.
Inventors
- 👤 Seigo Takasawa
- 👤 Yasushi Morikawa
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 20:17
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/14; C12N1/145; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2434; C12N9/2437; C12Y; C12Y302/00; C12Y302/01; C12Y302/01004; C12R; C12R2001/00; C12R2001/645; C12R2001/885; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/945
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
72 words
A process for the preparation of cellulase in which a microorganism of the genus Trichoderma, which is capable of producing cellulase, is cultured in order to accumulate cellulase in the culture medium and the resultant cellulase is harvested from the culture medium, characterised in that the said microorganism is a mutant strain of the genus Trichoderma, which exhibits increased inducibility of cellulase by L-sbrbose relative to that of a given parent strain.
Claim 2
Dependent Claim
72 words
A process for the preparation of cellulase in which a microorganism of the genus Trichoderma, which is capable of producing cellulase, is cultured in order to accumulate cellulase in the culture medium and the resultant cellulase is harvested from the culture medium, characterised in that the said microorganism is a mutant strain of the genus Trichoderma, which exhibits increased inducibility of cellulase by L-sbrbose relative to that of a given parent strain.
Claim 3
Dependent Claim
17 words
A process as claimed in claim 1, wherein said mutant strain is of the species Trichoderma reesei.
Claim 4
Dependent Claim
38 words
A process as claimed in claim 1 or claim 2, wherein said mutant strain is selected from Trichoderma reesei PC-1-4 (NRRL 15499), Trichoderma reesei PC-3-7 (NRRL 15500), Trichoderma reesei X-30 (NRRL 15501) and Trichoderma reesei X-31 (NRRL 15502).
Claim 5
Dependent Claim
50 words
A process as claimed in any of claims 1 to 3, wherein the carbon source of said medium comprises at least one member selected from filter papers, waste papers, bagasses, wheat straws, sweet sorghums, rice plant hulls and straws, soybean wastes, woody wastes and stalks of corns and Nepia grass.
Claim 6
Dependent Claim
33 words
A process as claimed in any of claims 1 to 4, wherein said medium contains a sugar source which comprises at least one member selected from sucrose, glucose, cane molasses, glycerol and cellobiose.
Claim 7
Dependent Claim
39 words
A process as claimed in any of claims 1 to 5, wherein culturing is effected at a temperature of from 20 to 32°C and a pH of from 3 to 6 for 4 to 10 days under aerobic conditions.
Claim 8
Dependent Claim
32 words
The mutant strain of Trichoderma reesei which is either PC-1-4 (NRRL 15499), PC-3-7 (NRRL 15500), X-30 (NRRL 15501) or X-31 (NRRL 15502) and mutants thereof which exhibit inducibility of cellulase by L-sorbose.
Claim 9
Dependent Claim
32 words
The mutant strain of Trichoderma reesei which is either PC-1-4 (NRRL 15499), PC-3-7 (NRRL 15500), X-30 (NRRL 15501) or X-31 (NRRL 15502) and mutants thereof which exhibit inducibility of cellulase by L-sorbose.
References Cited (Backward Citations)
No citation records indexed.
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