EP0132309A2
Expired / Lapsed
EP
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Novel regulatable eukaryotic promoter element
Assignee: Leland Stanford Junior University
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Filing: 1984-06-25
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Granted: —
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Expires: 2004-06-25
Abstract
Abstract Novel DNA sequences useful as portable divergent promoters for inducible simultaneous transcription. Specifically, galactose-induced promoters for GAL1-10 genes are provided on a single fragment for use with foreign genes, where simultaneous transcription can be induced with galactose. Constructs are prepared providing self-replication in a eukaryotic host.
Inventors
- 👤 Ronald W. Davis
- 👤 Henry Mark Johnston
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 05:30
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
17 words
A DNA fragment of less than about 2000bp having approximately symmetrically located the yeast GAL1-10 divergent promoters.
Claim 2
Dependent Claim
17 words
A DNA fragment of less than about 2000bp having approximately symmetrically located the yeast GAL1-10 divergent promoters.
Claim 3
Dependent Claim
25 words
A DNA fragment according to Claim 1, having termini at base pairs upstream from the terminal base pairs of the GALl and GAL10 structural genes.
Claim 4
Dependent Claim
16 words
A DNA fragment according to Claim 2, wherein said termini are upstream from the initiation codons.
Claim 5
Dependent Claim
20 words
A DNA fragment according to Claim 3, wherein said termini are within about 20 base pairs of said initiation codons.
Claim 6
Dependent Claim
24 words
A DNA construct comprising a DNA fragment having the_yeast GALl-10 divergent promoters and, in opposite orientations downstream from said promoters, genes foreign to yeast.
Claim 7
Dependent Claim
24 words
A DNA construct comprising a DNA fragment having the_yeast GALl-10 divergent promoters and, in opposite orientations downstream from said promoters, genes foreign to yeast.
Claim 8
Dependent Claim
12 words
A DNA construct according to Claim 5, having a yeast replication system.
Claim 9
Dependent Claim
13 words
A DNA construct according to Claim 5, having a marker recognized by yeast.
Claim 10
Dependent Claim
23 words
. 8. A DNA construct according to Claim 5, having a yeast replication system, a prokaryotic replication system and at least one marker.
Claim 11
Dependent Claim
14 words
A yeast host having an episomal element containing a construct according to Claim 5.
References Cited (Backward Citations)
No citation records indexed.
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