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EP0130523A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Immobilized nucleic acid probe and solid support for nucleic acids

Assignee: Molecular Diagnostics Inc
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Filing: 1984-06-25
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Granted: —
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Expires: 2004-06-25
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Abstract

Abstract A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a photochemically reactive intercalator compound or other nucleic acid-binding ligands, and (c) divalent radical chemically linking the substrate and the ligand (b). Specifically, a hydroxy group-containing solid substrate such as nitrocellulose paper is linked via a bifunctional reagent such as cyanogen bromide or 1,4-butanediol-diglycidyl ether to an amino-substituted angelicin or psoralen or ethidium bromide which in turn is photochemically linked to a nucleic acid. The resulting immobilized nucleic acid probe is capable of hybridizing with complementary nucleic acid fragments and is thereby useful in diagnostic assays.

Inventors

  • 👤 Nanibhushan Dattagupta
  • 👤 Donald M. Crothers

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-28 21:07
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6813; C12Q1/6834; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/548; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333

Claims Analysis

Total Claims: 12
Claim 1
Independent Claim 38 words
A solid support capable of binding a nucleic acid thereto upon irradiation, comprising (a) a solid substrate, (b) a photochemically reactive nucleic acid-binding ligand, and (c) a divalent radical chemically linking the substrate and the nucleic acid-binding ligand.
Claim 2
Dependent Claim 38 words
A solid support capable of binding a nucleic acid thereto upon irradiation, comprising (a) a solid substrate, (b) a photochemically reactive nucleic acid-binding ligand, and (c) a divalent radical chemically linking the substrate and the nucleic acid-binding ligand.
Claim 3
Dependent Claim 27 words
A support according to claim 1, characterized in that the nucleic acid-binding ligand is an intercalator compound selected from acridine dyes, phenanthridines, phenazines, furocoumarins, phenothiazines, and quinolines.
Claim 4
Dependent Claim 31 words
A support according to claim 1 or 2, characterized in that the substrate (a) in free state has free OH groups through which it is linked by the divalent radical (c).
Claim 5
Dependent Claim 22 words
A support according to any of claims 1 to 3, characterized in that the substrate (a) is cellulose or a cellulose ester.
Claim 6
Dependent Claim 26 words
A support according to any of claims 1 to 4, characterized in that the divalent radical (c) is derived from cyanogen bromide or from 1,4-butanediol-diglycidyl ether.
Claim 7
Dependent Claim 21 words
A support according to any of claims 1 to 5, characterized in that (b) is psoralen, angelicin, ethidium or derivatives thereof.
Claim 8
Dependent Claim 39 words
An immobilized nucleic acid probe comprising (a) a nucleic acid, (b) a nucleic acid binding ligand photochemically linked to the nucleic acid, and (c) a solid substrate chemically linked through a divalent radical to the nucleic acid-binding ligand (b).
Claim 9
Dependent Claim 39 words
An immobilized nucleic acid probe comprising (a) a nucleic acid, (b) a nucleic acid binding ligand photochemically linked to the nucleic acid, and (c) a solid substrate chemically linked through a divalent radical to the nucleic acid-binding ligand (b).
Claim 10
Dependent Claim 28 words
An immobilized probe according to claim 7, characterized in that the nucleic acid-binding ligand is an intercalator compound selected from acridine dyes, phenanthridines, phenazines, furocoumarins, phenothiazines, and quinolines.
Claim 11
Dependent Claim 31 words
An immobilized probe according to claim 7 or 8, characterized in that the substrate (c) in free state has free OH groups through which it is linked by the divalent radical.
Claim 12
Dependent Claim 22 words
An immobilized probe according to any of claims 7 to 9, characterized in that (b) is psoralen, angelicin, ethidium or derivatives thereof.

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