EP0124366A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method of measuring biological ligands
Assignee: Fujirebio Inc
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Filing: 1984-04-27
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Granted: —
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Expires: 2004-04-27
Abstract
Abstract A highly sensitive and specific method of measuring a biological ligand suitable as a clinical test for the determination of physiological substances and trace components in humoral fluid comprises allowing there to coexist in an aqueous medium a biologically active composition comprising an immobilised antibody phase or immobilised ligand phase on the one hand and an immobilised biotinyl enzyme or biotinyl enzyme inhibitor phase on the other hand, a water-soluble material combination of those of the ligand or the antibody on the one hand and the biotinyl enzyme inhibitor or the biotinyl enzyme on the other hand which are absent from the biologically active composition, and the ligand to be measured and measuring the residual biotinyl enzyme activity orthe biotinyl enzyme inhibitor activity and utilising the results as a measure of the ligand to be measured.
Inventors
- 👤 Yasushi Kasahara
- 👤 Yoshihiro Ashihara
- 👤 Hiromasa Suzuki
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 22:27
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/54306; G01N33/58; G01N33/581; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/813; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
192 words
A method of measuring a biological ligand, characterised by allowing there to coexist in an aqueous medium: (A) a biologically active composition comprising firstly an immobilised antibody phase whose antibody is capable of reacting with a-ligand (1) to be measured or an immobilised phase of a biological ligand (2) which is capable of reacting with said antibody and secondly an immobilised biotinyl enzyme phase or immobilised biotinyl enzyme inhibitor phase, (B) a water-soluble material combination of whichever of said ligand (2) or said antibody on the one hand and a biotinyl enzyme inhibitor or a biotinyl enzyme on the other hand are absent from the biologically active composition, the biotinyl enzyme inhibitor then being one which is capable of reacting with the biotinyl enzyme of the biologically active composition or the biotinyl enzyme then being one which is capable of reacting with the biotinyl enzyme inhibitor of the biologically active composition, and (C) the ligand (1) to be measured, and measuring the residual biotinyl enzyme activity or the residual biotinyl enzyme inhibitory activity of said biologically active composition or said aqueous solution and utilising the result as a measure of the ligand.
Claim 2
Independent Claim
192 words
A method of measuring a biological ligand, characterised by allowing there to coexist in an aqueous medium: (A) a biologically active composition comprising firstly an immobilised antibody phase whose antibody is capable of reacting with a-ligand (1) to be measured or an immobilised phase of a biological ligand (2) which is capable of reacting with said antibody and secondly an immobilised biotinyl enzyme phase or immobilised biotinyl enzyme inhibitor phase, (B) a water-soluble material combination of whichever of said ligand (2) or said antibody on the one hand and a biotinyl enzyme inhibitor or a biotinyl enzyme on the other hand are absent from the biologically active composition, the biotinyl enzyme inhibitor then being one which is capable of reacting with the biotinyl enzyme of the biologically active composition or the biotinyl enzyme then being one which is capable of reacting with the biotinyl enzyme inhibitor of the biologically active composition, and (C) the ligand (1) to be measured, and measuring the residual biotinyl enzyme activity or the residual biotinyl enzyme inhibitory activity of said biologically active composition or said aqueous solution and utilising the result as a measure of the ligand.
Claim 3
Dependent Claim
41 words
A method as claimed in claim 1, wherein said ligand (1) and said ligand (2) are selected from hormones derived from an endocrine gland, plasma proteins, viral antigens, bacteria, -fetoprotein, carcinoembyronic antigens, haptens and first antibodies in the double antibody method.
Claim 4
Dependent Claim
21 words
A method as claimed in claim 1 or 2, wherein said ligand (1) and said ligand (2) are the same substance.
Claim 5
Independent Claim
19 words
A method as claimed in any one of the preceding claims, wherein said antibody is a fragment of immunoglobulin.
Claim 6
Independent Claim
21 words
A method as claimed in any one of claims 1 to 3, wherein said antibody is produced from a warm-blooded animal.
Claim 7
Independent Claim
19 words
A method as claimed in any one of claims 1 to 3, wherein said antibody is a monoclonal antibody.
Claim 8
Independent Claim
34 words
A method as claimed in any one of the preceding claims, wherein said biotinyl enzyme is propionyl Co carboxylase, acetyl CoA carboxylase, pyruvate carboxylase, methylmalonyl CoA carboxylase, methylmalonyl CoA transcarboxylase or methylcrotonyl CoA carboxylase.
Claim 9
Independent Claim
28 words
A method as claimed in any one of the preceding claims, wherein said biotinyl enzyme inhibitor is avidin, streptoavidin or a derivative thereof capable of binding to biotin.
Claim 10
Independent Claim
45 words
A method as claimed in any one of the preceding claims, wherein the carrier material of said biologiclly active composition is a single block-copolymer or graft-copolymer, two polymer materials which are bound to each other or two separate bodies for immobilising respective biologically active components.
Claim 11
Independent Claim
32 words
A method as claimed in any one of the preceding claims, wherein said aqueous solution is at a pH 5 to 8 and has a temperature of from 15 to 45 C.
Claim 12
Dependent Claim
17 words
ll. A method as claimed in claim 10, wherein said aqueous solution is an aqueous buffer solution.
Claim 13
Independent Claim
40 words
A method as claimed in any one of the preceding claims, wherein, in measuring the biotinyl enzyme activity or the biotinyl enzyme inhibitory activity, a reaction is carried out which produces bioluminescence which is measured quantitatively using a photon counter.
References Cited (Backward Citations)
No citation records indexed.
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