EP0123903A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method for producing L-aspartic acid
Assignee: Tanabe Pharma Corp
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Filing: 1984-03-27
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Granted: —
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Expires: 2004-03-27
Abstract
Abstract A novel microorganism of the genus Serratia containing a hybrid plasmid prepared by integrating a plasmid with a deoxyribonucleic acid carrying the gene for aspartase which is obtained from a microorganism of the genus Serratia. An industrially advantageous method for producing L-aspartic acid comprising contacting a culture of the novel microorganism, microbial cells collected from the culture or a processed material of the microbial cells with fumaric acid and ammonia to produce L-aspartic acid and then collecting L-aspartic acid thus produced is also disclosed.
Inventors
- 👤 Masahiko Kisumi
- 👤 Tsutomu Takagi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 09:12
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/20; C12N; C12N9/00; C12N9/88
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
35 words
A microorganism of the genus Serratia containing a hybrid plasmid prepared by joining of a plasmid with a deoxyribonucleic acid carrying the gene for aspartase which is obtained from a microorganism of the genus Serratia.
Claim 2
Dependent Claim
35 words
A microorganism of the genus Serratia containing a hybrid plasmid prepared by joining of a plasmid with a deoxyribonucleic acid carrying the gene for aspartase which is obtained from a microorganism of the genus Serratia.
Claim 3
Dependent Claim
17 words
A microorganism of claim 1, wherein the plasmid to be joined with the deoxyribonucleic acid is pACYC177.
Claim 4
Dependent Claim
11 words
A microorganism of claim 2 being Serratia marcescens TA5001 (FERM BP-488).
Claim 5
Dependent Claim
17 words
The microorganism of claim 1, wherein the plasmid to be joined with the deoxyribonucleic acid is pBR322.
Claim 6
Dependent Claim
11 words
The microorganism of claim 4 being Serratia marcescens TA5002 (FERM BP-489).
Claim 7
Independent Claim
75 words
A method for producing L-aspartic acid comprising contacting a culture of a microorganism of the genus Serratia containing a hybrid plasmid prepared by joining a plasmid with a deoxyribonucleic acid carrying the gene for aspartase which is obtained from a microorganism of the genus Serratia, microbial cells collected from the culture or a processed material of the microbial cells with fumaric acid and ammonia to produce L-aspartic acid and then collecting L-aspartic acid thus produced.
Claim 8
Independent Claim
75 words
A method for producing L-aspartic acid comprising contacting a culture of a microorganism of the genus Serratia containing a hybrid plasmid prepared by joining a plasmid with a deoxyribonucleic acid carrying the gene for aspartase which is obtained from a microorganism of the genus Serratia, microbial cells collected from the culture or a processed material of the microbial cells with fumaric acid and ammonia to produce L-aspartic acid and then collecting L-aspartic acid thus produced.
Claim 9
Dependent Claim
18 words
A method of claim 6, wherein the microorganism containing the hybrid plasmid is Serratia marcescens TA5001 (FERM BP-488).
Claim 10
Dependent Claim
18 words
A method of claim 6, wherein the microorganism containing the hybrid plasmid is Serratia marcescens TA5002 (FERM BP-489).
Claim 11
Dependent Claim
18 words
A method of claim 6, wherein fumaric acid and ammonia is used in the form of ammonium fumarate.
References Cited (Backward Citations)
No citation records indexed.
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