Method for cell culture growth

Claim 1

A cell culture device comprising an elongated housing of impermeable material, closed at its ends by means of impermeable end plates, said housing on its interior having a plurality of axial ribs extending substantially throughout the length thereof, an inlet port in one of said end plates, an outlet port in the other end of said end plates, said ribs continuing radially in said other of said end plates, an impermeable spindle axially disposed in said housing and having axially disposed grooves in its periphery, a conical port secured to one end of said spindle and in communication with said inlet port and with said grooves, said spindle at its other end terminating in a conical tip axially disposed with respect to said outlet port with an annular space therebetween, and a spool of fiber helically wound on said spindle to substantially fill the interior of said housing into said ribs, whereby fluid entering said inlet port flows along said spindle grooves, passes through said fibrous spool, flows alongside said housing between said ribs, and then between said conical tip and said outlet port, said fiber having attached thereon cells of animal, vegetable, microbiological or artificial origin.

Claim 9

A method of cultivating cells in vitro which comprises: culturing said cells in the device of any of Claims 1-7.

Claim 10

A method of obtaining a substance which originates in cells of animal, plant, microbiological or artificial origin, by cultivating said cells in vitro while maintaining said cells immobilized, and thereafter collecting said products from said cells, the improvement wherein said cells are immobilized in a device according to any of Claims 1-7.