EP0122641A2
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EP
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Analytical reagent, analytical method, and multilayer chemical-analytical element
Assignee: Fujifilm Holdings Corp
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Filing: 1984-04-18
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Granted: —
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Expires: 2004-04-18
Abstract
Abstract A novel imidazole derivative type reagent for analysis of hydrogen peroxide, a method of analysis of hydrogen peroxide using the same, a method of analysis of peroxidase using the same, and a multilayer chemical-analytical element containing the same are disclosed. The imidazole derivative has the formula (I): wherein R' is an aryl group substituted with hydroxyl group at the ortho- or para-position, R 2 is an unsubstituted or substituted aryl group, and R 3 is an unsubstituted or substituted alkyl or an unsubstituted alkenyl group.
Inventors
- 👤 Akira Sato
- 👤 Chiaki Osada
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 14:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; G01N33/526; C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; C12Q2326/00
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
55 words
A reagent for analysis of hydrogen peroxide, which comprises an imidazole derivative having the formula (I): wherein R 1 is an aryl group substituted with hydroxyl group at the ortho- or para-position, R 2 is an unsubstituted or substituted aryl group, and R 3 is an unsubstituted or substituted alkyl or an unsubstituted alkenyl group.
Claim 2
Dependent Claim
55 words
A reagent for analysis of hydrogen peroxide, which comprises an imidazole derivative having the formula (I): wherein R 1 is an aryl group substituted with hydroxyl group at the ortho- or para-position, R 2 is an unsubstituted or substituted aryl group, and R 3 is an unsubstituted or substituted alkyl or an unsubstituted alkenyl group.
Claim 3
Dependent Claim
11 words
The reagent as claimed in claim 1, which further comprises peroxidase.
Claim 4
Independent Claim
67 words
A method of analysis of hydrogen peroxide which comprises: reacting hydrogen peroxide with an imidazole derivative having the formula (I): wherein R- is an aryl group substituted with hydroxyl group at the ortho- or para-position, R 2 is an unsubstituted or substituted aryl group, and R 3 is an unsubstituted or substituted alkyl or an unsubstituted alkenyl group, to form a color; and detecting the formed color.
Claim 5
Independent Claim
67 words
A method of analysis of hydrogen peroxide which comprises: reacting hydrogen peroxide with an imidazole derivative having the formula (I): wherein R- is an aryl group substituted with hydroxyl group at the ortho- or para-position, R 2 is an unsubstituted or substituted aryl group, and R 3 is an unsubstituted or substituted alkyl or an unsubstituted alkenyl group, to form a color; and detecting the formed color.
Claim 6
Dependent Claim
18 words
The method as claimed in claim 3, wherein the reaction is carried out in the presence of peroxidase.
Claim 7
Dependent Claim
28 words
The method as claimed in claim 3 or 4, wherein the detection of the color is conducted by colorimetry, and the analytical method is a quantitative analysis method.
Claim 8
Independent Claim
62 words
A method of analysis of peroxidase which comprises reacting an imidazole derivative having the formula (I) and hydrogen peroxide in the presence of peroxidase: wherein R is an aryl group substituted with hydroxyl group at the ortho- or para-position, R 2 is an unsubstituted or substituted aryl group, and R 3 is an unsubstituted or substituted alkyl or an unsubstituted alkenyl group.
Claim 9
Independent Claim
62 words
A method of analysis of peroxidase which comprises reacting an imidazole derivative having the formula (I) and hydrogen peroxide in the presence of peroxidase: wherein R is an aryl group substituted with hydroxyl group at the ortho- or para-position, R 2 is an unsubstituted or substituted aryl group, and R 3 is an unsubstituted or substituted alkyl or an unsubstituted alkenyl group.
Claim 10
Dependent Claim
76 words
A multilayer chemical-analytical element comprising a water-impermeable, light-transmissive support, a reagent layer and a porous layer superposed in this order, which is characterized in that an imidazole derivative having the formula (I) is contained in said reagent layer: wherein R is an aryl group substituted with hydroxyl group at the ortho- or para-position, R 2 is an unsubstituted or substituted aryl group, and R 3 is an unsubstituted or substituted alkyl or an unsubstituted alkenyl group.
Claim 11
Dependent Claim
76 words
A multilayer chemical-analytical element comprising a water-impermeable, light-transmissive support, a reagent layer and a porous layer superposed in this order, which is characterized in that an imidazole derivative having the formula (I) is contained in said reagent layer: wherein R is an aryl group substituted with hydroxyl group at the ortho- or para-position, R 2 is an unsubstituted or substituted aryl group, and R 3 is an unsubstituted or substituted alkyl or an unsubstituted alkenyl group.
Claim 12
Dependent Claim
11 words
The element as claimed in claim 7 which further contains peroxidase.
Claim 13
Dependent Claim
19 words
9: The element as claimed in claim 7 or 8, wherein said porous layer is a porous spreading layer.
Claim 14
Dependent Claim
22 words
The element as claimed in claim 7 or 8, wherein the porous layer is a porous layer having a definite surface area.
References Cited (Backward Citations)
No citation records indexed.
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