EP0119858A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
A method of and an apparatus for analysing a liquid specimen using a binding assay
Assignee: Hitachi Chemical Diagnostics Inc
•
Filing: 1984-03-16
•
Granted: —
•
Expires: 2004-03-16
Abstract
Abstract Method and apparatus for use in the diagnostic analysis of a liquid specimen through binding assays. The apparatus includes a rigid body having an elongated, shallow well formed in it, with a plurality of elongated strips, preferably in the form of cotton threads, stretched across the well in spaced relationship and generally perpendicular to the well's longitudinal axis. Each thread is coated with a separate binding conjugate such as an antigen or allergen. A cover plate that includes a thin plastic layer and an overlaying thin metallic layer covers the well to form an enclosed chamber having a specific volume. The metallic layer includes a separate elongated aperture in alignment with each coated thread. A liquid specimen is introduced into the chamber through one of two ports, for incubation with the coated threads, after which the specimen is removed and the reaction that occurred on each thread is determined.
Inventors
- 👤 William J. Sell
- 👤 David H. Riege
- 👤 Vincent A. Marinkovich
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-31 11:59
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N35/00; G01N35/10; G01N2035/1027; G01N2035/1048; G01N2035/1062
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
95 words
I. Apparatus for use in the diagnostic analysis of a liquid specimen through binding assays, which apparatus comprises a rigid body having an elongate well formed therein, a plurality of elongate members oriented in spaced relationship to each other across the well and generally transverse to the longitudinal axis of the well, each member having thereon a coating of a binding assay component, and the rigid body being adapted to receive in the well a liquid specimen for contact with the plurality of members and having a cover plate connectable therewith to enclose the well.
Claim 2
Dependent Claim
12 words
Apparatus according to claim I, wherein the elongate members are cotton threads.
Claim 3
Dependent Claim
42 words
Apparatus according to claim I or 2, wherein the rigid body has a substantially flat land surrounding the elongate well, and the cover plate is substantially flat and is secured to the land of the rigid body, to enclose the elongate well.
Claim 4
Dependent Claim
24 words
Apparatus according to claim 3, wherein each of the elongate members extends across the well from the land on opposite sides of the well.
Claim 5
Dependent Claim
34 words
Apparatus according to any one of claims I to 4, wherein the plurality of elongate members are held in tension across the elongate well and are each substantially straight and parallel with each other.
Claim 6
Dependent Claim
31 words
Apparatus according to any one of claims I to 5, wherein the cover plate comprises a thin metallic sheet having a separate elongate aperture aligned with each of the elongate members.
Claim 7
Dependent Claim
25 words
Apparatus according to claim 6, wherein the cover plate further comprises a thin plastics sheet disposed between the rigid body and the thin metallic sheet.
Claim 8
Dependent Claim
51 words
Apparatus according to claim 7, wherein the rigid body is formed of a plastics material, the plastics sheet and the metallic sheet of the cover plate are secured to each other by an adhesive, and the plastics sheet is secured to the land of the rigid body by a sonic weld.
Claim 9
Dependent Claim
14 words
Apparatus according to claim 7 or 8, wherein the thin plastics sheet is transparent.
Claim 10
Dependent Claim
38 words
Apparatus according to any one of claims I to 9, wherein the rigid body further comprises means defining a port at each end of the elongate well, to facilitate insertion and removal of a liquid in the well.
Claim 11
Dependent Claim
28 words
li. Apparatus according to claim 10, wherein the rigid body further comprises a pipette projection in alignment with the port located at one end of the elongate well.
Claim 12
Dependent Claim
170 words
Apparatus for use in the allergy screening of a liquid specimen through binding assays, the apparatus comprising: a rigid plastics body having an elongate, shallow well formed therein, and a substantially flat land surrounding the well, a plurality of cotton threads stretched tautly across the elongate well from the land on its opposite sides, the threads being oriented in spaced parallel relationship substantially perpendicular to the longitudinal axis of the well, each thread being coated with a different allergen or group of allergens, a substantially flat cover plate secured to the land of the rigid body, to enclose the well, the cover plate comprising a thin, transparent plastics sheet overlaying to the land of the rigid body, and a thin metallic sheet overlaying the plastics sheet and having a separate elongate aperture aligned with each of the cotton threads, and means defining a port at each end of the well, to facilitate insertion and removal of a liquid specimen in the well, for contact with the plurality of cotton threads.
Claim 13
Independent Claim
97 words
A method for the diagnostic analysis of a liquid specimen through binding assays, which method comprises the steps of coating each of a plurality of elongate members with a binding assay component, positioning the plurality of elongate members in a predetermined spaced relationship to each other in a chamber of a prescribed volume formed within a rigid body, placing a liquid specimen in the chamber for incubation with the coated members, removing the liquid specimen from the chamber, and measuring the degree of reaction that has occurred between the liquid specimen and each of the coated members.
Claim 14
Dependent Claim
70 words
A method according to claim 13, wherein the chamber is defined by an elongate, shallow well formed in the rigid body, the step of positioning includes a step of stretching the elongated members across the well, generally transverse to the longitudinal axis of the well, and the method further comprises the step of securing a cover plate to the rigid body, to enclose the well and thereby form the chamber.
Claim 15
Dependent Claim
19 words
A method according to claim 14, wherein the cover plate is secured to the rigid body by sonic welding.
Claim 16
Dependent Claim
47 words
A method according to claim 14 or 15, wherein the step of securing includes a step of positioning a thin metallic plate over the well formed in the rigid body, the plate having a separate elongate aperture in alignment with each of the plurality of elongate members.
Claim 17
Independent Claim
18 words
A method according to any one of claims 13 to 16, wherein the elongate members are cotton threads.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.