EP0119702A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Synthetic polypeptides from viral oncogenes
Assignee: Individual
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Filing: 1984-02-02
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Granted: —
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Expires: 2004-02-02
Abstract
Abstract Synthetic polypeptides and their conjugates bound to a carrier are disclosed along with antibodies raised thereto, and methods of their use. The amino acid residue sequence of the synthetic polypeptides correspond substantially to an amino acid sequence of a determinant of an oncoprotein encoded by an oncogenic virus. That determinant domain is vicinal to but exclusive of an active site of the oncoprotein. The synthetic polypeptide or its conjugate is capable of inducing the production of antibodies which can inhibit a reaction at an oncoprotein active site, immunoprecipitate the oncoprotein and react competitively with an admixture containing the synthetic polypeptide or its conjugate and another oncoprotein having a homologous determinant domain.
Inventors
- 👤 Arup Sen
- 👤 Richard A. Lerner
- 👤 Richard A. Houghten
- 👤 James L. Bittle
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 22:30
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/57575; C; C07; C07K; C07K14/00; C07K14/82; C07K16/00; C07K16/18; C07K16/32; A; A61; A61K; A61K39/00
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
87 words
A synthetic polypeptide having an amino acid residue sequence corresponding substantially to an amino acid residue sequence of a determinant domain of an oncoprotein encoded by an oncogenic virus, said determinant domain being vicinal to but exclusive of an active site of said oncoprotein, and said synthetic polypeptide having the capacity, alone or as a conjugate of said polypeptide bound to a carrier, of inducing the production of antibodies when injected into a host, said antibodies inhibiting a reaction at said active site of said oncoprotein. ,
Claim 2
Dependent Claim
87 words
A synthetic polypeptide having an amino acid residue sequence corresponding substantially to an amino acid residue sequence of a determinant domain of an oncoprotein encoded by an oncogenic virus, said determinant domain being vicinal to but exclusive of an active site of said oncoprotein, and said synthetic polypeptide having the capacity, alone or as a conjugate of said polypeptide bound to a carrier, of inducing the production of antibodies when injected into a host, said antibodies inhibiting a reaction at said active site of said oncoprotein. ,
Claim 3
Dependent Claim
16 words
The synthetic polypeptide according to claim 1 containing about 10 to about 40 amino acid residues.
Claim 4
Dependent Claim
19 words
The synthetic polypeptide according to claim 1 wherein said sequence contains about 10 to about 25 amino acid residues.
Claim 5
Dependent Claim
168 words
The synthetic polypeptide according to claim 1 having an amino acid sequence containing about 10 to about 25 amino acid residues and corresponding substantially to an amino acid residue sequence of a first determinant domain of a first oncoprotein produced by cells transformed by an oncogenic virus, said first determinant domain being vicinal to but exclusive of a phosphotransfer catalysis site of said oncoprotein, and said synthetic polypeptide having the capacity, alone or as a conjugate of said polypeptide bound to a carrier, of inducing the production of antibodies when injected into a host animal, wherein said antibodies: (a) immunoprecipitate said oncoprotein; (b) inhibit a phosphotransfer reaction catalyzed by said oncoprotein at said phosphotransfer site; and (c) react competitively with an admixture containing said polypeptide or said conjugate and a second oncoprotein present in a body component of a tumor-bearing animal, said second oncoprotein having a determinant domain homologous to said first domain, and the tumor of said animal being other than that induced by said oncogenic virus.
Claim 6
Dependent Claim
12 words
The synthetic polypeptide of any preceding claim bonded to a carrier molecule.
Claim 7
Dependent Claim
28 words
An antibody raised to a synthetic antigen, said synthetic antigen comprising a synthetic polypeptide of any preceding claim, or a conjugate of said polypeptide bound to a carrier.
Claim 8
Dependent Claim
48 words
The antibody according to claim 6 that: (a) inhibits a phosphotransfer reaction at an active site of a first . oncoprotein, . (b) is capable of precipitating said first oncoprotein, and (c) immunoreacts with a second oncoprotein having a determinant domain homologous to that of said first oncoprotein.
Claim 9
Dependent Claim
139 words
A diagnostic reagent system for assaying for the presence of an increased amount of a first oncoprotein encoded by an oncogene normally present in cells as compared to the amount of said first oncoprotein normally present, comprising in separate containers: (a) a first reagent that contains in biologically active form a synthetic polypeptide of any of claims 1 to 4; and (b) a second reagent in biologically active form including idiotype-containing polyamides raised to said synthetic polypeptide or its conjugate, along with indicating groups; said first and second reagents when admixed in predetermined amounts in the presence of a predetermined amount of a body component to be assayed providing an amount of immunoreaction, the amount of said immunoreaction being different from a known immunoreaction amount when an increased amount of said first oncoprotein is present in said body component.
Claim 10
Dependent Claim
63 words
A diagnostic reagent comprising idiotype-containing polyamides that are individually bonded to indicating groups, wherein said idiotype-containing polyamides (a) are raised to a synthetic polypeptide of any of claims 1 to 4, or to a conjugate of said synthetic polypeptide bound to a carrier; and (b) immunoreact with a second oncoprotein having an antigenic domain homologous to said determinant domain of said first oncoprotein.
Claim 11
Independent Claim
98 words
A method of inhibiting the growth of neoplastic cells comprising the steps of: (a) . providing neoplastic cells having oncoproteins on their surfaces; (b) contacting said cells with an effective amount of idiotype-containing polyamides raised to a synthetic polypeptide of any of claims 1 to 4, or to a conjugate of said polypeptide bound to a carrier, said synthetic polypeptide having an amino acid sequence that is homologous to a determinant domain of said oncoproteins produced by said neoplastic cells; and maintaining said contact to form immune complexes between said neoplastic cell surface oncoproteins and said idiotype-contaihing polyamides.
Claim 12
Dependent Claim
16 words
The method according to claim 10 wherein said idiotype-containing polyamides include a cytotoxic drug bonded thereto.
Claim 13
Independent Claim
78 words
A method for detecting the presence of neoplastic disease in an animal comprising the steps of (a) providing a body component from said animal; (b) admixing said body component with a composition including idiotype-containing polyamides raised to a synthetic polypeptide of any of claims 1 to 4, or to a conjugate of said polypeptide bound to a carrier; and (c) determining the presence, in said admixture, of an immunological reaction between said body component and said idiotype-containing polyamides.
Claim 14
Dependent Claim
149 words
A composition comprising idiotype-containing polyamides that are individually bonded to indicating groups, wherein said idiotype-containing polyamides (i) are raised to a synthetic polypeptide of any of claims 1 to 4, or to a conjugate of said synthetic polypeptide bound to a carrier; (ii) inhibit a reaction at an active site of said oncoprotein; and (iii) immunoreact with a second oncoprotein having an antigenic determinant domain homologous to said domain of said first oncoprotein, for use in a method of locating a neoplastic tumor in an animal in vivo, said method comprising the steps of: (a) injecting said composition into the bloodstream of a neoplastic tumor-bearing animal; (b) incubating said animal for a predetermined period of time sufficient for the indicating group-bonded polypeptide to form a complex on the surface of said tumor; and (c) scanning said animal with a means for detecting the location of said complexed indicating groups.
Claim 15
Dependent Claim
33 words
A vaccine comprising an effective amount of a synthetic polypeptide of any of claims 1 to 4, or a conjugate of said polypeptide bound to a carrier, dispersed in a physiologically tolerable vehicle.
Claim 16
Dependent Claim
18 words
A vaccine according to claim 14, for use in a method of immunising an animal against neoplastic growth.
Claim 17
Dependent Claim
45 words
An idiotype-containing polyamide raised to a synthetic polypeptide of any of claims 1 to 4, or to a conjugate of said polypeptide bound to a carrier, for use in a method of inhibiting the growth of neoplastic cells according to claim 10 or claim 11.
References Cited (Backward Citations)
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