EP0119020B1
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Method of preparing liposomes and products produced thereby
Assignee: ER Squibb and Sons LLC
•
Filing: 1984-02-14
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Granted: 1988-06-01
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Expires: 2004-02-14
Abstract
No abstract record available.
Inventors
- 👤 Frank Ridgway
- 👤 Nicholas I. Payne
- 👤 Peter Timmins
- 👤 Cheryl Vanessa Groom
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 03:54
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1277
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
108 words
A method for preparing a stable liposome precursor in the form of a thin film of liposomal components coated on a particulate carrier material, which comprises forming a solution in a suitable solvent of at least one liposome-forming lipid, optionally, at least one biologically active compound, and optionally, at least one adjuvant which imparts advantageous properties to the final liposome preparation, and coating a granular free-flowing carrier material which is substantially insoluble in said solvent, with the so-formed solution to form a thin film of liposomal components on said carrier material, wherein the carrier material is a physiologically acceptable carrier material which has a relatively high water solubility.
Claim 2
Independent Claim
108 words
A method for preparing a stable liposome precursor in the form of a thin film of liposomal components coated on a particulate carrier material, which comprises forming a solution in a suitable solvent of at least one liposome-forming lipid, optionally, at least one biologically active compound, and optionally, at least one adjuvant which imparts advantageous properties to the final liposome preparation, and coating a granular free-flowing carrier material which is substantially insoluble in said solvent, with the so-formed solution to form a thin film of liposomal components on said carrier material, wherein the carrier material is a physiologically acceptable carrier material which has a relatively high water solubility.
Claim 3
Dependent Claim
45 words
The method as defined in claim 1 wherein said carrier material has a water-solubility in excess of 10% by weight, a rapid dissolution rate in water, and will form an isotonic solution in water in a concentration of from about 1 to about 10% w/v.
Claim 4
Dependent Claim
21 words
The method as defined in Claim 1 or 2 wherein said carrier material is a water-soluble carrier suitable for intravenous use.
Claim 5
Dependent Claim
22 words
The method as defined in Claim 3 wherein said carrier material is sorbitol, mannitol, or xylitol or a naturally occurring amino acid.
Claim 6
Dependent Claim
13 words
The method as defined in Claim 4 wherein said carrier material is sorbitol.
Claim 7
Dependent Claim
18 words
The method as defined in Claim 1 wherein the carrier material is sodium chloride, lactose dextrose or sucrose.
Claim 8
Independent Claim
32 words
The method as defined in any preceding claim wherein the solution is formed by dissolving said lipid, optionally, said biologically active compound and, optionally, said adjuvant in one or more organic solvents.
Claim 9
Dependent Claim
25 words
The method as defined in Claim 7 wherein the organic solvent is ethanol, methanol, chloroform, dichloromethane, diethylether, carbon tetrachloride, ethyl acetate, dioxane or cyc- lochexane.
Claim 10
Dependent Claim
15 words
The method as defined in Claim 8 wherein the solvent is methanol, ethanol or chloroform.
Claim 11
Dependent Claim
42 words
A modification of the method as defined in any one of Claims 1-6 wherein the coating solution is formed by dissolving the optional biologically active compound and optional adjuvant in said lipid, said lipid being of the low melting point liposome-forming type.
Claim 12
Independent Claim
38 words
The method as defined in any one of Claims 1-9 wherein the particulate carrier material is coated with said solution by suspending the carrier material in the solution of liposomal components and spray drying the coated carrier material.
Claim 13
Independent Claim
14 words
The method as defined in any preceding claim wherein the lipid is a phospholipid.
Claim 14
Dependent Claim
16 words
The method as defined in Claim 12 wherein the phospholipid is a natural or synthetic lecithin.
Claim 15
Independent Claim
30 words
The method as defined in any preceding claim wherein said solution includes a biologically active compound which is a medicament, contrast agent, enzyme, hormone, marker compound or NMR imaging agent.
Claim 16
Independent Claim
20 words
The method as defined in any preceding claim wherein the adjuvant is egg phosphatidic acid, dicetyl phosphate, or stearyl amine.
Claim 17
Independent Claim
16 words
The method as defined in any one of Claims 1-14 wherein the adjuvant is a sterol.
Claim 18
Dependent Claim
30 words
The method as defined in Claim 15 wherein the adjuvant also comprises caprolactam and/or a sterol selected from cholesterol, phytosterol, ergosterol, sitosterol, sitosterol pyroglutamate, 7- dehydrocholesterol, lanosterol and mixtures thereof.
Claim 19
Independent Claim
16 words
The method as defined in any preceding claim wherein the biologically active compound is amphotericin B.
Claim 20
Dependent Claim
29 words
The method as defined in Claim 1 wherein the biologically active compound is amphotericin B, said carrier material is sorbitol and said adjuvant includes a sterol which is ergosterol.
Claim 21
Dependent Claim
27 words
The method as defined in Claim 1 wherein the lipid is egg lecithin, the adjuvant is ergosterol, cholesterol or dicetyl phosphate, and the carrier material is sorbitol.
Claim 22
Dependent Claim
25 words
The method as defined in Claim 1 wherein the lipid is egg lecithin, the adjuvant is ergosterol or cholesterol, and the carrier material is lactose.
Claim 23
Dependent Claim
65 words
A stable liposome precursor which when mixed with water forms a liposomal preparation, comprising a particulate carrier material, said carrier material being coated with a thin film comprised of at least one liposome-forming lipid, optionally at least one biologically active compound and, optionally, at least one adjuvant which imparts advantageous properties to the final liposome preparation wherein the carrier material is a water-soluble particulate material.
Claim 24
Dependent Claim
65 words
A stable liposome precursor which when mixed with water forms a liposomal preparation, comprising a particulate carrier material, said carrier material being coated with a thin film comprised of at least one liposome-forming lipid, optionally at least one biologically active compound and, optionally, at least one adjuvant which imparts advantageous properties to the final liposome preparation wherein the carrier material is a water-soluble particulate material.
Claim 25
Dependent Claim
39 words
The stable liposome precursor as defined in Claim 22 wherein the carrier material is a free-flowing physiologically acceptable carrier material which will form an isotonic solution in water in a concentration of from about 1 to about 10% w/v.
Claim 26
Dependent Claim
15 words
The stable liposome precursor as defined in Claim 23 wherein the carrier material is sorbitol.
Claim 27
Dependent Claim
20 words
The stable liposome precursor as defined in any one of Claims 22-24 wherein the biologically active compound is amphotericin B.
Claim 28
Dependent Claim
65 words
A stable liposome precursor which when mixed with water forms a liposomal preparation, comprising a water-soluble carrier material coated with a thin film comprised of at least one liposome-forming lipid, optionally, at least one biologically active compound and, optionally, at least one adjuvant which imparts advantageous properties to the final liposome preparation, prepared by the method as defined in any one of Claims 1 -21.
Claim 29
Independent Claim
23 words
A method for preparing a liposome preparation which comprises exposing the liposome precursor as defined in any one of Claims 22-26 to water.
References Cited (Backward Citations)
No citation records indexed.
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