Patent US3665421A successfully refreshed from Google Patents.
EP0117032A1
Expired / Lapsed
EP
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Rapid analysis of ethanol in body fluids
Assignee: Alcoholism and Drug Addiction Research Foundation
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Filing: 1984-01-09
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Granted: —
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Expires: 2004-01-09
Abstract
Abstract Novel solid compositions are provided for assaying the levels of lower alcohols in body fluids. A carrier is impregnated with two or more enzymes, the first of which reduces a reducible agent in the presence of the alcohol. A chromogen is provided which reacts with either the reducer or the oxidized compound in the presence of a second enzyme or reagent, depending on the system, to produce a clinically significant indication of the alcohol present. A quantitative determination may be made by the provision of a competitive inhibitor to the first enzyme to provide a graduated response in colour according to the concentration of alcohol., Acetaldehyde trapping agents, buffers and enzyme stabilizers are provided as required.
Inventors
- 👤 Bhushan Madhodass Kapur
- 👤 Yedy Israel
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 08:59
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/30; C12Q1/32
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
104 words
A solid composition for determining the presence of a lower alcohol in a biological fluid such as urine, saliva or blood comprising a solid carrier having the following constituents in effective amounts: a first enzyme for effecting oxidation of said alcohol to produce the corresponding aldehyde and a reduction product, a chromogen reactable with said aldehyde or said reduced product in the presence of second or third agents to effect a colour change indicative of an alcohol presence in the body fluid; an enzyme chosen from the group of said second or third agents or both; and a buffer to maintain a predetermined pH.
Claim 2
Dependent Claim
104 words
A solid composition for determining the presence of a lower alcohol in a biological fluid such as urine, saliva or blood comprising a solid carrier having the following constituents in effective amounts: a first enzyme for effecting oxidation of said alcohol to produce the corresponding aldehyde and a reduction product, a chromogen reactable with said aldehyde or said reduced product in the presence of second or third agents to effect a colour change indicative of an alcohol presence in the body fluid; an enzyme chosen from the group of said second or third agents or both; and a buffer to maintain a predetermined pH.
Claim 3
Dependent Claim
23 words
A solid composition as claimed in claim 1 further including a reducible compound reactable with ethanol in the presence of said first enzyme.
Claim 4
Dependent Claim
15 words
A solid composition as claimed in claim 2 wherein said first enzyme is alcohol dehydrogenase.
Claim 5
Dependent Claim
16 words
A solid composition as claimed in claim 3 wherein said reducible compound is nicotinamide adenine dinucleotide.
Claim 6
Dependent Claim
33 words
A solid composition as claimed in claim 1 wherein said composition further includes an inhibitor competitive of said first enzyme whereby said colour change is indicative of the quantity of said reduced compound.
Claim 7
Dependent Claim
14 words
A solid composition as claimed in claim 3 further including an acetaldehyde trapping agent.
Claim 8
Dependent Claim
35 words
A solid composition as claimed in claim 5 wherein said first enzyme is alcohol dehydrogenase and said inhibitor is chosen from the group comprising a pyrazole, a halogenated pyrazole, an alkyl pyrazole or a steroid.
Claim 9
Dependent Claim
41 words
A solid composition as claimed in claim 5 or 7 wherein said second agent is chosen from the group comprising diaphorase, phenazine or thiazine and said chromagen is chosen from the group comprising a tetrazolium salt, 2-6-dichlorophenol, indophenol or methylene blue.
Claim 10
Dependent Claim
24 words
A solid composition as claimed in claim 1 wherein said first enzyme is alcohol oxidase and includes a competitive inhibitor for said first enzyme.
Claim 11
Dependent Claim
53 words
A solid composition as claimed in claim 1 further including a reagent chosen from the group comprising barium oxide or barium hydroxide which produces hydrogen peroxide with water, and a nucleotide reactable with said aldehyde to produce a reduced product reactable with said chromogen in the presence of one of said further agents.
Claim 12
Dependent Claim
21 words
A composition as claimed in claim 10 wherein said third agent is chosen from the group comprising diaphorase, phenazine or thiazine.
Claim 13
Dependent Claim
15 words
A composition as claimed in claim 10 or 11 further including a sulphydryl reducing agent.
Claim 14
Dependent Claim
15 words
A composition as claimed in any of claims 9 to 12 including an inert protein.
Claim 15
Dependent Claim
120 words
An inert carrier including a first discrete area impregnated with a first alcohol determining impregnant and a second discrete area impregnated with a second alcohol determining impregnant said first impregnant being chosen from the group including in effective amounts, nicotinamide adenine dinucleotide, alcohol dehydrogenase, a chromagen reactive in the presence of a second agent chosen from the group comprising diaphorase, phenazine or thiazine; one of diaphorase, phenazine or thiazine, an acetaldehyde trapping agent, a buffer to -maintain a predetermined pH and means for stabilizing said enzymes; and a second alcohol determining impregnant including, in effective amounts; alcohol oxidase; a chromogen reactive in the presence of peroxidase; peroxidase; a buffer to maintain a predetermined pH and means to stabilize said enzymes.
Claim 16
Dependent Claim
120 words
An inert carrier including a first discrete area impregnated with a first alcohol determining impregnant and a second discrete area impregnated with a second alcohol determining impregnant said first impregnant being chosen from the group including in effective amounts, nicotinamide adenine dinucleotide, alcohol dehydrogenase, a chromagen reactive in the presence of a second agent chosen from the group comprising diaphorase, phenazine or thiazine; one of diaphorase, phenazine or thiazine, an acetaldehyde trapping agent, a buffer to -maintain a predetermined pH and means for stabilizing said enzymes; and a second alcohol determining impregnant including, in effective amounts; alcohol oxidase; a chromogen reactive in the presence of peroxidase; peroxidase; a buffer to maintain a predetermined pH and means to stabilize said enzymes.
Claim 17
Dependent Claim
46 words
An inert carrier as claimed in claim 1.4 wherein said first composition further includes inhibitors, competitive with said alcohol dehydrogenase or said alcohol oxidase; a chromogen reactive in the presence of peroxidase; peroxidase; a buffer to maintain a predetermined pH and means to stabilize said enzymes.
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