EP0116784A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Atrial natriuretic factor
Assignee: Queen's University
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Filing: 1983-12-22
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Granted: —
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Expires: 2003-12-22
Abstract
Abstract Diuretic and natriuretic extracts, which have been characterized as peptide in nature, have been obtained by homogenization of mammalian heart atria with an aqueous solution of a lower carboxylic acid and may be prepared synthetically. After precipitation of impurities by pH adjustment, the extract may be further purified chromatographically. Extracts injected into test rats resulted in 30-40 fold increases in sodium and chloride excretions within 5 - 10 minutes of injection. Urine Volume rose 10-15 fold and potassium excretion doubled. The response was complete in 20 minutes and no similar changes in renal function were observed following injection of a similarly obtained ventricular extract.
Inventors
- 👤 Adolfo Jose De Bold
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-27 15:55
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/58; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
65 words
A process for extracting a diuretic and natriuretic factor from mammalian heart atria comprising: (a) homogenizing mammalian heart atria in an aqueous solution containing a lower carboxylic acid to thereby extract said factor into said solution; (b) adjusting the pH of said solution within the range 4.5 to 7.6 to thereby precipitate impurities therefrom; and (c) chromatographically purifying said natriuretic factor remaining in said solution.
Claim 2
Dependent Claim
65 words
A process for extracting a diuretic and natriuretic factor from mammalian heart atria comprising: (a) homogenizing mammalian heart atria in an aqueous solution containing a lower carboxylic acid to thereby extract said factor into said solution; (b) adjusting the pH of said solution within the range 4.5 to 7.6 to thereby precipitate impurities therefrom; and (c) chromatographically purifying said natriuretic factor remaining in said solution.
Claim 3
Dependent Claim
15 words
A process as claimed in claim 1 wherein said lower carboxylic acid is acetic acid.
Claim 4
Dependent Claim
24 words
A process as claimed in claim 1 wherein said chromatographic step is selected from gel chromatography, ion exchange chromatography and reverse phase liquid chromatography.
Claim 5
Dependent Claim
22 words
A process as claimed in claim 1 wherein the volume of said solution is reduced before said chromatographic purification step by freeze-drying.
Claim 6
Dependent Claim
19 words
A process as claimed in claim 1 wherein said aqueous solution additionally contains a mineral acid and a salt.
Claim 7
Dependent Claim
20 words
A process as claimed in claim 5 wherein said mineral acid is hydrochloric acid and said salt is sodium chloride.
Claim 8
Dependent Claim
16 words
A process as claimed in claim 1, wherein said mammalian heart atria are rat heart atria.
Claim 9
Dependent Claim
14 words
A diuretic and natriuretic composition obtained by carboxylic acid extraction from mammalian heart atria.
Claim 10
Dependent Claim
14 words
A diuretic and natriuretic composition obtained by carboxylic acid extraction from mammalian heart atria.
Claim 11
Dependent Claim
14 words
A composition as claimed in claim 8 obtained by extraction from rat heart atria.
Claim 12
Dependent Claim
26 words
A peptide composition having diuretic and natriuretic properties, obtained by carboxylic acid extraction from mammalian heart atria, characterized by an amino acid composition (in residues) comprising:
Claim 13
Dependent Claim
26 words
A peptide composition having diuretic and natriuretic properties, obtained by carboxylic acid extraction from mammalian heart atria, characterized by an amino acid composition (in residues) comprising:
Claim 14
Dependent Claim
21 words
A peptide composition as claimed in claim 10 having a molecular weight in the range from about 5,100 to about 5,300.
Claim 15
Dependent Claim
69 words
A peptide composition having diuretic and natriuretic properties, characterized by an amino acid sequence comprising: Serine - Leucine - Arginine - Arginine - Serine - Serine - Cysteine - Phenylalanine - Glycine - Glycine - Arginine - Isoleucine - Aspartic acid - Arginine - Isoleucine - Glycine - Alanine - Glutamine - Serine - Glycine. - Leucine - Glycine - Cysteine - Asparagine - Serine - Phenylalanine-(Arginine) - Tyrosine.
Claim 16
Dependent Claim
69 words
A peptide composition having diuretic and natriuretic properties, characterized by an amino acid sequence comprising: Serine - Leucine - Arginine - Arginine - Serine - Serine - Cysteine - Phenylalanine - Glycine - Glycine - Arginine - Isoleucine - Aspartic acid - Arginine - Isoleucine - Glycine - Alanine - Glutamine - Serine - Glycine. - Leucine - Glycine - Cysteine - Asparagine - Serine - Phenylalanine-(Arginine) - Tyrosine.
Claim 17
Dependent Claim
18 words
A peptide composition as claimed in claim 12 further characterized by a disulfide bond between said cysteine groups.
Claim 18
Dependent Claim
17 words
A peptide composition as claimed in claim 12 or 13 having a molecular weight of about 3,500.
References Cited (Backward Citations)
No citation records indexed.
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