Signal processing method in autoradiography

Claim 2

A signal processing method in autoradiography for determination of base sequence of DNA or DNA fragment, employing radioactively labeled cleavage products obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element, comprising: 1) at least two sets of reference mixtures consisting essentially of guanine specific cleavage products, adenine specific cleavage products, thymine specific cleavage products and cytosine specific cleavage products; and 2) at least one group of cleavage products or mixture thereof containing at least one kind of the base specific cleavage products, both 1) and 2) being resolved one-dimensionally in such parallel relation that resolved rows of said two sets of reference mixtures 1) sandwitch at least one resolved row of the group 2) to form at least three resolved rows on a support medium, which comprises a process including: (1) detecting reference sampling points in each reference row, the reference row being the resolved row of the reference mixture; (2) detecting sampling points in the resolved row other than the reference rows; (3) joining the corresponding reference sampling points among said plural reference rows to fix plural continuous lines comprising straight lines, polygonal lines or curved lines; and I (4) comparing and identifying said sampling points in the resolved row other than the reference rows utilizing said continuous lines; said process being applied to a digital signal corresponding to an autoradiograph having the locational information on groups of radioactively labeled cleavage products, said digital signal being obtained by exposing a radiosensitive material to radiation emitted by said groups of radioactively labeled cleavage products on the support medium to record the autoradiograph of the groups of radioactively labeled cleavage products on the radiosensitive material, and reading out said autoradiograph photoelectrically.

Claim 3

The signal processing method in autoradiography as claimed in claim 1, wherein at least three resolved rows of the reference mixtures 1) are so provided on the support medium that two resolved rows of the reference mixtures in close relation to each other sandwitch at least one resolved row of the group 2).

Claim 4

The signal processing method in autoradiography as claimed in claim 1 or 2, wherein the sampling points are detected by processing the digital signal on each scanning line for the plural resolved rows respectively with at least one processing selected from the group consisting of smoothing and threshold-processing.

Claim 5

The signal processing method in autoradiography as claimed in claim 1 or 2, wherein the employed cleavage products of DNA or DNA fragment comprise at least five groups including: 1) guanine specific cleavage products + adenine specific cleavage products + thymine specific cleavage products + cytosine specific cleavage products; 2) base specific cleavage products containing guanine specific cleavage products; 3) base specific cleavage products containing adenine specific cleavage products; 4) base specific cleavage products containing thymine specific cleavage products; and 5) base specific cleavage products containing cytosine specific cleavage products.

Claim 6

A signal processing method in autoradiography for determination of base sequence of DNA or DNA fragment, employing radioactively labeled cleavage products obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element, comprising: 1) at least two sets of specimens for synthesizing a reference row, the set comprising at least two groups of cleavage products or mixture thereof, the groups being in such combination as to totally contain guanine specific cleavage products, adenine specific cleavage products, thymine specific cleavage products and cytosine specific cleavage products; and 2) at least one group of cleavage products or mixture thereof containing at least one kind of the base specific cleavage products, both 1) and 2) being resolved one-dimensionally in such parallel relation that resolved rows of said two sets of specimens for synthesizing a reference row 1) which respectively comprise two or more rows sandwitch at least one resolved row of the group 2) to form at least five resolved rows on a support medium, which comprises a process including: (1) detecting sampling points in each resolved row; (2) synthesizing a reference row from the two or more of resolved rows of the specimen with respect to each set of specimen and determining reference sampling points; (3) joining the corresponding reference sampling points among said plural reference rows to fix plural continuous lines comprising straight lines, polygonal lines or curved lines; and (4) comparing and identifying said sampling points in each resolved row utilizing said continuous lines; said process being applied to a digital signal corresponding to an autoradiograph having the locational information on groups of radioactively labeled cleavage products, said digital signal being obtained by exposing a radiosensitive material to radiation emitted by said groups of radioactively labeled cleavage products on the support medium to record the autoradiograph of the groups of radioactively labeled cleavage products on the radiosensitive material, and reading out said autoradiograph photoelectrically.

Claim 7

A signal processing method in autoradiography for determination of base sequence of DNA or DNA fragment, employing radioactively labeled cleavage products obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element, comprising: 1) at least two sets of specimens for synthesizing a reference row, the set comprising at least two groups of cleavage products or mixture thereof, the groups being in such combination as to totally contain guanine specific cleavage products, adenine specific cleavage products, thymine specific cleavage products and cytosine specific cleavage products; and 2) at least one group of cleavage products or mixture thereof containing at least one kind of the base specific cleavage products, both 1) and 2) being resolved one-dimensionally in such parallel relation that resolved rows of said two sets of specimens for synthesizing a reference row 1) which respectively comprise two or more rows sandwitch at least one resolved row of the group 2) to form at least five resolved rows on a support medium, which comprises a process including: (1) detecting sampling points in each resolved row; (2) synthesizing a reference row from the two or more of resolved rows of the specimen with respect to each set of specimen and determining reference sampling points; (3) joining the corresponding reference sampling points among said plural reference rows to fix plural continuous lines comprising straight lines, polygonal lines or curved lines; and (4) comparing and identifying said sampling points in each resolved row utilizing said continuous lines; said process being applied to a digital signal corresponding to an autoradiograph having the locational information on groups of radioactively labeled cleavage products, said digital signal being obtained by exposing a radiosensitive material to radiation emitted by said groups of radioactively labeled cleavage products on the support medium to record the autoradiograph of the groups of radioactively labeled cleavage products on the radiosensitive material, and reading out said autoradiograph photoelectrically.

Claim 8

The signal processing method in autoradiography as claimed in claim 5, wherein resolved rows of at least three sets of the specimens for synthesizing a reference row 1) which respectively comprise two or more rows are so provided on the support medium that said resolved rows of the two sets of the specimens in close relation to each other sandwitch at least one resolved row of the group 2).

Claim 9

The signal processing method in autoradiography as claimed in claim 5 or 6, wherein the sampling points are detected by processing the digital signal on each scanning line for the plural resolved rows respectively with at least one processing selected from the group consisting of smoothing and threshold-processing.

Claim 10

The signal processing method in autoradiography as claimed in claim 5 or 6, wherein the set of specimen for synthesizing a reference row consists essentially of two groups of mixtures of base specific cleavage products including: 1) guanine specific cleavage products + adenine specific cleavage products; and 2) thymine specific cleavage products + cytosine specific cleavage products.

Claim 11

A signal processing method in autoradiography for determination of base sequence of DNA or DNA fragment, employing radioactively labeled cleavage products obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element, comprising: 1) at least one set of a reference mixture consisting essentially of guanine specific cleavage products, adenine specific cleavage products, thymine specific cleavage products and cytosine specific cleavage products; 2) at least one set of specimen for synthesizing a reference row, the set comprising at least two groups of cleavage products or mixture thereof, the groups being in such combination as to totally include guanine specific cleavage products, adenine specific cleavage products, thymine specific cleavage products and cytosine specific cleavage products; and 3) at least one group of cleavage products or mixture thereof containing at least one kind of the base specific cleavage products, these 1), 2) and 2) being resolved one-dimensionally in such parallel relation that a resolved row of the reference mixture 1) and two or more of resolved rows of the specimen for synthesizing a reference row 2) sandwitch at least one resolved row of the group 3) to form at least four resolved rows on a support medium, which comprises a process including: (1) detecting sampling points in each resolved row; (2) assigning both the sampling points in a reference row being the resolved row of the reference mixture, and the sampling points in a reference row synthesized from the two or more of resolved rows of the specimen to reference sampling points; (3) joining the corresponding reference sampling points among said plural reference rows to fix plural continuous lines comprising straight lines, polygonal lines or curved lines; and (4) comparing and identifying said sampling points in each resolved row utilizing said continuous lines; said process being applied to a digital signal corresponding to an autoradiograph having the locational information on groups of radioactively labeled cleavage products, said digital, signal being obtained by exposing a radiosensitive material to radiation emitted by said groups of radioactively labeled cleavage products on the support medium to record the autoradiograph of the groups of radioactively labeled cleavage products on the radiosensitive material, and reading out said autoradiograph photoelectrically.

Claim 12

A signal processing method in autoradiography for determination of base sequence of DNA or DNA fragment, employing radioactively labeled cleavage products obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element, comprising: 1) at least one set of a reference mixture consisting essentially of guanine specific cleavage products, adenine specific cleavage products, thymine specific cleavage products and cytosine specific cleavage products; 2) at least one set of specimen for synthesizing a reference row, the set comprising at least two groups of cleavage products or mixture thereof, the groups being in such combination as to totally include guanine specific cleavage products, adenine specific cleavage products, thymine specific cleavage products and cytosine specific cleavage products; and 3) at least one group of cleavage products or mixture thereof containing at least one kind of the base specific cleavage products, these 1), 2) and 2) being resolved one-dimensionally in such parallel relation that a resolved row of the reference mixture 1) and two or more of resolved rows of the specimen for synthesizing a reference row 2) sandwitch at least one resolved row of the group 3) to form at least four resolved rows on a support medium, which comprises a process including: (1) detecting sampling points in each resolved row; (2) assigning both the sampling points in a reference row being the resolved row of the reference mixture, and the sampling points in a reference row synthesized from the two or more of resolved rows of the specimen to reference sampling points; (3) joining the corresponding reference sampling points among said plural reference rows to fix plural continuous lines comprising straight lines, polygonal lines or curved lines; and (4) comparing and identifying said sampling points in each resolved row utilizing said continuous lines; said process being applied to a digital signal corresponding to an autoradiograph having the locational information on groups of radioactively labeled cleavage products, said digital, signal being obtained by exposing a radiosensitive material to radiation emitted by said groups of radioactively labeled cleavage products on the support medium to record the autoradiograph of the groups of radioactively labeled cleavage products on the radiosensitive material, and reading out said autoradiograph photoelectrically.

Claim 13

The signal processing method in autoradiography as claimed in claim 9, wherein at least three sets of resolved rows in combination of a resolved row of the reference mixture 1) and two or more of resolved rows of the specimen for synthesizing a reference row 2) are so provided on the support medium that said two sets of. resolved rows in close relation to each other sandwitch at least one resolved row of the group 3).

Claim 14

The signal processing method in autoradiography as claimed in claim 9 or 10, wherein the sampling points are detected by processing the digital signal on each scanning line for the plural resolved rows respectively with a at least one processing selected from smoothing and threshold-processing.

Claim 15

The signal processing method in autoradiography as claimed in claim 9 or 10, wherein the set of specimen for synthesizing a reference row 2) consists essentially of two groups of mixtures of base specific cleavage products including: 1) guanine specific cleavage products . + adenine specific cleavage products; and 2) thymine specific cleavage products + cytosine specific cleavage products.