Signal processing method in autoradiography

Claim 2

A signal processing method in autcradiograohy for determination of base sequence cf DNA or DNA fragment, employing at least two groups of radioactively labeled cleavage products obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element, comprising: 1) a mixture cf cleavage products comprising four kinds of base specific cleavage products consisting essentially of guanine specific cleavage products, adenine specific cleavage products, thymine specific cleavage products and cytosine specific cleavage products; and 2) cleavage products containing at least one kind of base specific cleavage products, both being resolved one-dimensionally in parallel relation to form at least two resolved rows on a support medium, which comprises a process including: (1) detecting reference sampling points in a reference row, the reference row being the resolved row of the mixture 1) of cleavage products comprising four kinds of base specific cleavage products; (2) detecting sampling points in the resolved row other than the reference row; and (3) comparing the reference sampling points in the reference row with sampling points in a resolved row adjacent to said reference row to identify the sampling points in the adjacent resolved row, said process being applied to digital signal corresponding to an autoradiograph having the locational information on said groups of radioactively labeled cleavage products, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the groups of radioactively labeled cleavage products on the support medium to record the autoradiograph of the groups of radoactively labeled cleavage products on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically.

Claim 3

The signal processing method in autoradiography as claimed in claim 1, wherein the sampling points are detected by processing the digital signal on respective scanning lines for the plural resolved rows with at least one processing selected from the group consisting of smoothing and threshold-processing.

Claim 4

A signal processing method in autoradiography for determination of base sequence of DNA or DNA fragment, employing at least three groups of radioactively labeled cleavage products obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element, comprising: 1) a mixture of cleavage products comprising four kinds of base specific cleavage products consisting essentially of guanine specific cleavage products, adenine specific cleavage products, thymine specific cleavage products and cytosine specific cleavage products; and 2) at least two groups of cleavage products in which one group of cleavage proudcts contains at least one kind of base specific cleavage products different from base specific cleavage products contained in the other group of cleavage products, both 1) and 2) being resolved one-dimensionally in parallel relation to form at least three resolved rows on a support medium, which comprises a process including: (1) detecting reference sampling points in a reference row, the reference row being the resolved row of the mixture of cleavage products comprising four kinds of base specific cleavage products; (2) detecting sampling points in the resolved rows other than the reference row; (3) comparing the reference sampling points in the reference row with sampling points in a resolved row adjacent to said reference row to identify the sampling points in the adjacent resolved row and determining imaginary reference sampling points in said resolved row based on thus indentified sampling points; and (4) comparing thus determined imaginary reference sampling points with sampling points in a resolved row adjacent to said resolved row for which the reference sampling points are determined in the process (3) to identify the sampling points in the adjacent resolved row, said process being applied to digital signal corresponding to an autoradiograph having the locational information on said groups of radioactively labeled cleavage products, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the groups of radioactively labeled cleavage products on the support medium to record the autoradiograph of the groups of radoactively labeled cleavage products on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically.

Claim 5

A signal processing method in autoradiography for determination of base sequence of DNA or DNA fragment, employing at least three groups of radioactively labeled cleavage products obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element, comprising: 1) a mixture of cleavage products comprising four kinds of base specific cleavage products consisting essentially of guanine specific cleavage products, adenine specific cleavage products, thymine specific cleavage products and cytosine specific cleavage products; and 2) at least two groups of cleavage products in which one group of cleavage proudcts contains at least one kind of base specific cleavage products different from base specific cleavage products contained in the other group of cleavage products, both 1) and 2) being resolved one-dimensionally in parallel relation to form at least three resolved rows on a support medium, which comprises a process including: (1) detecting reference sampling points in a reference row, the reference row being the resolved row of the mixture of cleavage products comprising four kinds of base specific cleavage products; (2) detecting sampling points in the resolved rows other than the reference row; (3) comparing the reference sampling points in the reference row with sampling points in a resolved row adjacent to said reference row to identify the sampling points in the adjacent resolved row and determining imaginary reference sampling points in said resolved row based on thus indentified sampling points; and (4) comparing thus determined imaginary reference sampling points with sampling points in a resolved row adjacent to said resolved row for which the reference sampling points are determined in the process (3) to identify the sampling points in the adjacent resolved row, said process being applied to digital signal corresponding to an autoradiograph having the locational information on said groups of radioactively labeled cleavage products, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the groups of radioactively labeled cleavage products on the support medium to record the autoradiograph of the groups of radoactively labeled cleavage products on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically.

Claim 6

The signal processing method in autoradiography as claimed in claim 3, wherein the resolved rows other than the reference row are provided in both sides of the reference row on the support medium; and the process (3) is carried out on said resolved rows provided in both sides of the reference row.

Claim 7

The signal processing method in autoradiography as claimed in claim 3 or 4, wherein the sampling points are detected by processing the digital signal on respective scanning lines for the plural resolved rows with at least one processing selected from the group consisting of smoothing and threshold-processing.

Claim 8

The signal processing method in autoradiography as claimed in claims 3 or 4, wherein the employed cleavage products of DNA or DNA fragment contain at least five groups of cleavage products consisting of: 1) guanine specific cleavage products + adenine specific cleavage products + thymine specific cleavage products + cytosine specific cleavage products; 2) base specific cleavage products containing guanine specific cleavage products; 3) base specific cleavage products containing adenine specific cleavage products; 4) base specific cleavage products containing thymine specific cleavage products; and 5) base specifically cleabed product containing cytosine specific cleavage products.

Claim 9

A signal processing method in autoradiography for determination of base sequence of DNA or DNA fragment, employing at least four groups of radioactively labeled cleavage products obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element, comprising: 1) base specific cleavage products containing at least guanine specific cleavage products; 2) base specific cleavage products containing at least adenine specific cleavage products; 3) base specific cleavage products containing at least thymine specific cleavage products; and 4) base specifically cleabed product containing at least cytosine specific cleavage products, being resolved one-dimensionally in parallel relation to each other to form resolved rows on a support medium, which comprises a process including: (1) detecting sampling points in each resolved row; (2) synthesizing a reference row from the plural resolved rows and assigning the sampling points in the reference row to reference sampling points; (3) comparing the reference sampling points in the reference row with sampling points in a resolved row adjacent to the reference row employed for the synthesis of said reference row to identify the sampling points in the adjacent resolved row and determining imaginary reference sampling points in said resolved row based on thus indentified sampling points; and (4) comparing thus determined imaginary reference sampling points with sampling points in a resolved row adjacent to said resolved row for which the reference sampling points are determined in the process (3) to identify the sampling points in the adjacent resolved row, said process being applied to digital signal corresponding to an autoradiograph having the locational information on said groups of radioactively labeled cleavage products, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the groups of radioactively labeled cleavage products on the support medium to record the autoradiograph of the groups of radioactively labeled cleavage products on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically.

Claim 10

A signal processing method in autoradiography for determination of base sequence of DNA or DNA fragment, employing at least four groups of radioactively labeled cleavage products obtained by specifically cleaving the DNA or DNA fragment labeled with a radioactive element, comprising: 1) base specific cleavage products containing at least guanine specific cleavage products; 2) base specific cleavage products containing at least adenine specific cleavage products; 3) base specific cleavage products containing at least thymine specific cleavage products; and 4) base specifically cleabed product containing at least cytosine specific cleavage products, being resolved one-dimensionally in parallel relation to each other to form resolved rows on a support medium, which comprises a process including: (1) detecting sampling points in each resolved row; (2) synthesizing a reference row from the plural resolved rows and assigning the sampling points in the reference row to reference sampling points; (3) comparing the reference sampling points in the reference row with sampling points in a resolved row adjacent to the reference row employed for the synthesis of said reference row to identify the sampling points in the adjacent resolved row and determining imaginary reference sampling points in said resolved row based on thus indentified sampling points; and (4) comparing thus determined imaginary reference sampling points with sampling points in a resolved row adjacent to said resolved row for which the reference sampling points are determined in the process (3) to identify the sampling points in the adjacent resolved row, said process being applied to digital signal corresponding to an autoradiograph having the locational information on said groups of radioactively labeled cleavage products, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the groups of radioactively labeled cleavage products on the support medium to record the autoradiograph of the groups of radioactively labeled cleavage products on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically.

Claim 11

The signal processing method in autoradiography as claimed in claim 7, wherein resolved rows are provided in both sides of the resolved rows used for the systhesis of reference row on the support medium; and the process (3) is carried out for said resolved rows provided in both sides thereof.

Claim 12

The signal processing method in autoradiography as claimed in claim 7 or 8, wherein the sampling points are detected by processing the digital signal on respective scanning lines for the plural resolved rows with at least one processing selected from the group consisting of smoothing and threshold-processing.

Claim 13

The signal processing method in autoradiography as claimed in claim 7 or 8, wherein the employed cleavage products of DNA or DNA fragment contain at least four groups of cleavage products consisting of: 1) guanine specific cleavage products; 2) guanine specific cleavage products + adenine specific cleavage products; 3) thymine specific cleavage products + cytosine specific cleavage products; 4) cytosine specific cleavage products.