Signal processing method in autoradiography

Claim 2

A signal processing method in autoradiography for obtaining information on one dimensional location of radioactively labeled substances distributed in at least one dimensional direction on a support medium, in the form of symbol, numeral or combination thereof, which comprises a process including: (1) determining one dimensional scanning line for signal processing; and (2) detecting sampling points on said scanning line, said process being applied to digital signal corresponding to an autoradiograph having the locational information on the radioactively labeled substances, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by said radioactively labeled substances on the support medium to record the autoradiograph of the radioactively labeled substances on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically.

Claim 3

The signal processing method in autoradiography as claimed in claim 1, in which all points on said scanning line at which the signal exhibits a maximum level are assigned to sampling points on the scanning line.

Claim 4

The signal processing method in autoradiography as claimed in claim 1 or 2, in which said radioactively labeled substances distributed on the support medium are biopolymers, derivatives thereof, or cleavage products thereof, labeled with a radioactive element and resolved on the support medium in one dimensional direction.

Claim 5

The signal processing method in autoradiography as claimed in claim 3, in which said biopolymers are nucleic acids, derivatives thereof or cleavage products thereof; and said symbol, numeral or combination thereof obtained by said signal processing represents base sequence thereof.

Claim 6

A signal processing method in autoradiography for obtaining information on one dimensional location of groups of radioactively labeled substances arranged in plural rows and distributed respectively in at least one dimensional direction on a support medium, in the form of symbol, numeral or combination thereof, which comprises a process including: (1) determining on each of the plural rows one dimensional scanning line for signal processing; (2) detecting on each of the plural rows sampling points on said scanning line; and (3) comparing and identifying said sampling points detected in the plural rows, said process being applied to digital signal corresponding to an autoradiograph having the locational information on the groups of radioactively labeled substances, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the groups of radioactively labeled substances on the support medium to record the autoradiograph of the groups of radioactively labeled substances on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically.

Claim 7

A signal processing method in autoradiography for obtaining information on one dimensional location of groups of radioactively labeled substances arranged in plural rows and distributed respectively in at least one dimensional direction on a support medium, in the form of symbol, numeral or combination thereof, which comprises a process including: (1) determining on each of the plural rows one dimensional scanning line for signal processing; (2) detecting on each of the plural rows sampling points on said scanning line; and (3) comparing and identifying said sampling points detected in the plural rows, said process being applied to digital signal corresponding to an autoradiograph having the locational information on the groups of radioactively labeled substances, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the groups of radioactively labeled substances on the support medium to record the autoradiograph of the groups of radioactively labeled substances on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically.

Claim 8

The signal processing method in autoradiography as claimed in claim 5, in which all points on said scanning line at which the signal exhibits maximum levels are assigned to sampling points on the scanning line.

Claim 9

The signal processing method in autoradiography as claimed in claim 5 or 6, in which said comparing and identifying of the sampling points are performed by numerical calculation on the positions of sampling points on the scanning lines among the plural rows.

Claim 10

The signal processing method in autoradiography as claimed in claim 5 or 6, in which said groups of radioactively labeled substances distributed on the support medium are biopolymers, derivatives thereof, or cleavage products thereof, labeled with a radioactive element and resolved on the support medium in one dimensional direction.

Claim 11

The signal processing method in autoradiography as claimed in claim 8, in which said biopolymers are nucleic acids, derivatives thereof or cleavage products thereof; and said symbol, numeral or combination thereof obtained by said signal processing represents base sequence thereof.

Claim 12

A signal processing method in autoradiography for determining base sequence of DNA or DNA fragment, employing at least four groups of base specific cleavage products consisting of: 1) base specific cleavage products including at least guanine specific cleavage products; 2) base specific cleavage product including at least adenine specific cleavage products; 3) base specific cleavage product including at least cytosine specific cleavage products; and 4) base specific cleavage product including at least thymine specific cleavage products, which are obtained by specific cleavage of the DNA or DNA fragment which has been labeled with a radioactive element, and resolved respectively in one dimensional direction to form resolved rows on a support medium, which comprises a process including: (1) determining on each of the resolved rows one dimensional scanning line for signal processing; (2) detecting on each of the resolved rows sampling points on said scanning line; and (3) comparing and identifying the positions of said sampling points on the scanning lines among the resolved rows to obtain locational information on guanine, adenine, thymine and cytosine, said process being applied to digital signal corresponding to an autoradiograph having the locational information on the groups of radioactively labeled cleavage products, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the groups of radioactively labeled cleavage products on the support medium to record the autoradiograph of the groups of radioactively labeled cleavage products on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically.

Claim 13

A signal processing method in autoradiography for determining base sequence of DNA or DNA fragment, employing at least four groups of base specific cleavage products consisting of: 1) base specific cleavage products including at least guanine specific cleavage products; 2) base specific cleavage product including at least adenine specific cleavage products; 3) base specific cleavage product including at least cytosine specific cleavage products; and 4) base specific cleavage product including at least thymine specific cleavage products, which are obtained by specific cleavage of the DNA or DNA fragment which has been labeled with a radioactive element, and resolved respectively in one dimensional direction to form resolved rows on a support medium, which comprises a process including: (1) determining on each of the resolved rows one dimensional scanning line for signal processing; (2) detecting on each of the resolved rows sampling points on said scanning line; and (3) comparing and identifying the positions of said sampling points on the scanning lines among the resolved rows to obtain locational information on guanine, adenine, thymine and cytosine, said process being applied to digital signal corresponding to an autoradiograph having the locational information on the groups of radioactively labeled cleavage products, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the groups of radioactively labeled cleavage products on the support medium to record the autoradiograph of the groups of radioactively labeled cleavage products on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically.

Claim 14

The signal processing method in autoradiography as claimed in claim 10, in which said groups of base specific cleavage products of DNA or DNA fragment include at least four groups consisting of: 1) guanine specific cleavage products; 2) guanine specific cleavage products + adenine specific cleavage products; 3) thymine specific cleavage products + cytosine specific cleavage products; and 4) cytosine specific cleavage products.

Claim 15

A signal processing method in autoradiography for obtaining information on one dimensional location of radioactively labeled substances distributed in at least one dimensional direction on a support medium, in the form of symbol, numeral or combination thereof.and a visible image, which comprises: (I) a process for obtaining the information in the form of symbol, numeral or combination thereof including: (1) determining one dimensional scanning line for signal processing; and (2) detecting sampling points on said scanning line, said process being applied to digital signal corresponding to an autoradiograph having the locational information on the radioactively labeled substances, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by said radioactively labeled substances on the support medium to record the autoradiograph of the radioactively labeled substances on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically, and (II) a process for reproducing the visible image from an electric or digital signal corresponding to said autoradiograph obtained by detecting said stimulated emission photoelectrically.

Claim 16

A signal processing method in autoradiography for obtaining information on one dimensional location of radioactively labeled substances distributed in at least one dimensional direction on a support medium, in the form of symbol, numeral or combination thereof.and a visible image, which comprises: (I) a process for obtaining the information in the form of symbol, numeral or combination thereof including: (1) determining one dimensional scanning line for signal processing; and (2) detecting sampling points on said scanning line, said process being applied to digital signal corresponding to an autoradiograph having the locational information on the radioactively labeled substances, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by said radioactively labeled substances on the support medium to record the autoradiograph of the radioactively labeled substances on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically, and (II) a process for reproducing the visible image from an electric or digital signal corresponding to said autoradiograph obtained by detecting said stimulated emission photoelectrically.

Claim 17

The signal processing method in autoradiogra- )hy as claimed in claim 12, in which all points on said scanning line at which the signal exhibits maximum Levels are assigned to sampling points on the scanning Line.

Claim 18

The signal processing method in autoradiography as claimed in claim 12 or 13, in which said radioactively labeled substances distributed on the support medium are biopolymers, derivatives thereof, or cleavage products thereof, labeled with a radioactive element and resolved on the support medium in one dimensional direction.

Claim 19

The signal processing method in autoradiography as claimed in claim 14, in which said biopolymers are nucleic acids, derivatives thereof or cleavage products thereof; and said symbol, numeral or combination thereof obtained by said signal processing represents base sequence thereof.

Claim 20

A signal processing method in autoradiography for obtaining information on one dimensional location of groups of radioactively labeled substances arranged in plural rows and distributed respectively in at least one dimensional direction on a support medium, in the form of symbol, numeral or combination thereof and a visible image, which comprises: (I) a process for obtaining the information in the form of symbol, numeral or combination including: (1) determining on each of the plural rows one dimensional scanning line for signal processing; (2) detecting on each of the plural rows sampling points on said scanning line; and (3) comparing and identifying said sampling points detected in the plural rows, said process being applied to digital signal corresponding to an autoradiograph having the locational information on the groups of radioactively labeled substances, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the groups of radioactively labeled substances on the support medium to record the autoradiograph of the groups of radioactively labeled substances on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically, and (II) a process for reproducing the visible image from an electric or digital signal corresponding to said autoradiograph obtained by detecting said stimulated emission photoelectrically.

Claim 21

A signal processing method in autoradiography for obtaining information on one dimensional location of groups of radioactively labeled substances arranged in plural rows and distributed respectively in at least one dimensional direction on a support medium, in the form of symbol, numeral or combination thereof and a visible image, which comprises: (I) a process for obtaining the information in the form of symbol, numeral or combination including: (1) determining on each of the plural rows one dimensional scanning line for signal processing; (2) detecting on each of the plural rows sampling points on said scanning line; and (3) comparing and identifying said sampling points detected in the plural rows, said process being applied to digital signal corresponding to an autoradiograph having the locational information on the groups of radioactively labeled substances, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the groups of radioactively labeled substances on the support medium to record the autoradiograph of the groups of radioactively labeled substances on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically, and (II) a process for reproducing the visible image from an electric or digital signal corresponding to said autoradiograph obtained by detecting said stimulated emission photoelectrically.

Claim 22

The signal processing method in autoradiography as claimed in claim 16, in which all points on said scanning line at which the signal exhibits maximum levels are assigned to sampling points on the scanning line.

Claim 23

The signal processing method in autoradiography as claimed in claim 16 or 17, in which said comparing and identifying of the sampling points are performed by numerical calculation on the positions of sampling points on the scanning lines among the plural rows.

Claim 24

The signal processing method in autoradiography as claimed in claim 16 or 17, in which said groups of radioactively labeled substances distributed on the support medium are biopolymers, derivatives thereof, or cleavage products thereof, labeled with a radioactive element and resolved on the support medium in one dimensional direction.

Claim 25

The signal processing method in autoradiography as claimed in claim 19, in which said biopolymers are nucleic acids, derivatives thereof or cleavage products thereof; and said symbol, numeral or combination thereof obtained by said signal processing represents base sequence thereof.

Claim 26

A signal processing method in autoradiography for determining base sequence of DNA or DNA fragment, employing at least four groups of base specific cleavage products consisting of: 1) base specific cleavage products including at least guanine specific cleavage products; 2) base specific cleavage product including at least adenine specific cleavage products; 3) base specific cleavage product including at least cytosine specific cleavage products; and 4) base specific cleavage product including at least thymine specific cleavage products, which are obtained by specific cleavage of the DNA or DNA fragment which has been labeled with a radioactive element, and resolved respectively in one dimensional direction to form resolved rows on a support medium, which comprises: (I) a processes including: (1) determining on each of the resolved rows one dimensional scanning line for signal processing; (2) detecting on each of the resolved rows sampling points on said scanning line; and (3) comparing and identifying the positions of said sampling points on the scanning lines among the resolved rows to obtain locational information on guanine, adenine, thymine and cytosine, said processes being applied to digital signal corresponding to an autoradiograph having the locational information on the group of radioactively labeled cleavage products, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the group of radioactively labeled cleavage products on the support medium to record the autoradiograph of the group of radioactively labeled cleavage products on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically, and (II) a process for reproducing the visible image from an electric or digital signal corresponding to said autoradiograph obtained by detecting said stimulated emission photoelectrically.

Claim 27

A signal processing method in autoradiography for determining base sequence of DNA or DNA fragment, employing at least four groups of base specific cleavage products consisting of: 1) base specific cleavage products including at least guanine specific cleavage products; 2) base specific cleavage product including at least adenine specific cleavage products; 3) base specific cleavage product including at least cytosine specific cleavage products; and 4) base specific cleavage product including at least thymine specific cleavage products, which are obtained by specific cleavage of the DNA or DNA fragment which has been labeled with a radioactive element, and resolved respectively in one dimensional direction to form resolved rows on a support medium, which comprises: (I) a processes including: (1) determining on each of the resolved rows one dimensional scanning line for signal processing; (2) detecting on each of the resolved rows sampling points on said scanning line; and (3) comparing and identifying the positions of said sampling points on the scanning lines among the resolved rows to obtain locational information on guanine, adenine, thymine and cytosine, said processes being applied to digital signal corresponding to an autoradiograph having the locational information on the group of radioactively labeled cleavage products, said digital signal being obtained by causing a stimulable phosphor sheet to absorb radiation energy emitted by the group of radioactively labeled cleavage products on the support medium to record the autoradiograph of the group of radioactively labeled cleavage products on the stimulable phosphor sheet, scanning said stimulable phosphor sheet with an electromagnetic wave to release at least a portion of radiation energy stored in said phosphor sheet in the form of the autoradiograph as stimulated emission, and detecting said stimulated emission photoelectrically, and (II) a process for reproducing the visible image from an electric or digital signal corresponding to said autoradiograph obtained by detecting said stimulated emission photoelectrically.

Claim 28

The signal processing method in autoradiography as claimed in claim 21, in which said groups of base specific cleavage products of DNA or DNA fragment include at least four groups consisting of: 1) guanine specific cleavage products; 2) guanine specific cleavage products + adenine specific cleavage products; 3) thymine specific cleavage products + cytosine specific cleavage products; and 4) cytosine specific cleavage products.