EP0111344A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Anti-interleukin-2 monoclonal antibodies
Assignee: Memorial Sloan Kettering Cancer Center
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Filing: 1983-12-13
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Granted: —
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Expires: 2003-12-13
Abstract
Abstract Monoclonal antibodies recognizing interleukin-2 (IL-2) of various species (human, rat, mouse) are disclosed. These antibodies are useful in purification of IL-2 from biological sample as well as in separating IL-2 into various sub-species. Immunoassay of IL-2 and identification of cells containing IL-2 are made possible by these monoclonal antibodies. The monoclonal antibodies are also of therapeutic use in the treatment of syndromes associated with hyperproduction of interleukin-2 including hyperimmune syndromes GvH, acute lymphoblastic leukemia, T-cell lymphoma or leukemia.
Inventors
- 👤 Hans-Joachim Feickert
- 👤 Karl Welte
- 👤 Roland Mertelsmann
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 23:03
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/24; C07K16/244; C07K16/246; C07K14/00; C07K14/435; C07K14/52; C07K14/54; C07K14/55; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/6863; G01N33/6869
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
4 words
Monoclonal antibodies recognizing IL2.
Claim 2
Dependent Claim
4 words
Monoclonal antibodies recognizing IL2.
Claim 3
Dependent Claim
10 words
The monoclonal antibody of Claim 1 of immunoglobulin sub-class A.
Claim 4
Dependent Claim
16 words
Antibody-producing hybridoma cell line characterized by the production of the antibodies as claimed in Claim 1.
Claim 5
Dependent Claim
23 words
Antibody-producing hybridoma cell line of Claim 2 formed by fusing a myeloma derived cell strain with splenocytes-derived from BALB/C mice immunized with interleukin-2.
Claim 6
Dependent Claim
21 words
Antibody-producing hybridoma cell line of Claim 4 wherein said human interleukin-2 comprises fractions of molecular weight between about 14K and 17K.
Claim 7
Dependent Claim
11 words
Monoclonal antibody FW-4 having a molecular weight of about 160,000 daltons.
Claim 8
Dependent Claim
11 words
Monoclonal antibody FW-4 having a molecular weight of about 160,000 daltons.
Claim 9
Dependent Claim
41 words
Method of purifying interleukin-2 from a sample comprising contacting said sample with monoclonal antibody of Claim 1 under conditions suitable for forming an interleukin 2-anti-interleukin-2 antibody complex; separating said complex from said sample; and treating said complex to separate interleukin-2 therefrom.
Claim 10
Dependent Claim
26 words
Method of separating sub-species of interleukin-2 from non-homogeneous mixtures of interleukin-2 comprising contacting said mixtures with monoclonal antibody of Claim 1 under conditions suitable for reaction..
Claim 11
Dependent Claim
17 words
Immunoassay of interleukin-2 in a sample comprising contacting said sample with the monoclonal antibody of Claim 1.
Claim 12
Dependent Claim
9 words
Immunoassay of Claim 9 wherein said immunoassay is radioimmunoassay.
Claim 13
Dependent Claim
10 words
Immunoassay of Claim 9 wherein said immunoassay is. enzyme-linked immunoassay.
Claim 14
Dependent Claim
27 words
A method of inhibiting proliferation of interleukin-2 dependent cultured human and mouse cell lines comprising contacting said cultured cell lines with the monoclonal antibody of Claim 1..
Claim 15
Dependent Claim
21 words
Method of treating an individual having a syndrome associated with hyperproduction of interleukin-2 comprising administering anti-interleukin-1 monoclonal antibodies to said individual.
Claim 16
Dependent Claim
21 words
Method of treating an individual having a syndrome associated with hyperproduction of interleukin-2 comprising administering anti-interleukin-1 monoclonal antibodies to said individual.
Claim 17
Dependent Claim
14 words
Method of Claim 13 wherein said monoclonal antibodies are coupled to a cytotoxic agent.
Claim 18
Dependent Claim
11 words
Method of Claim 13 wherein said syndrome is a hyperimmune syndrome.
Claim 19
Dependent Claim
17 words
Method of Claim 15 wherein said hyperimmune syndrome is GvH, acute lymphoblastic leukemia, T-cell lymphoma or leukemia.
Claim 20
Dependent Claim
28 words
Method for locating cells which contain interleukin-2 in a mixture of cells comprising immunoassay of said mixture with the monoclonal antibodies of Claim 1 and observing-the reaction site.
Claim 21
Dependent Claim
19 words
The assay of Claim 17 wherein said monoclonal antibody is coupled to a fluorescent, chromophoric or a radioactive substance.
References Cited (Backward Citations)
No citation records indexed.
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