EP0110186A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Aminomethylfluorescein derivatives
Assignee: Abbott Laboratories
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Filing: 1983-11-04
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Granted: —
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Expires: 2003-11-04
Abstract
Abstract This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. This disclosure also relates to a novel class of tracer compounds employed as reagents in fluorescence polarization immunoassays.
Inventors
- 👤 Curtis Lee Kirkemo
- 👤 Mohammed Tyebjl Shipchandler
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-02 22:10
CPC Classifications
C; C07; C07D; C07D493/00; C07D493/02; C07D493/10; C07J; C07J41/00; C07J41/0005; C07J41/0016; C09; C09B; C09B11/00; C09B11/04; C09B11/10; C09B11/24; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/815; Y10S436/817
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
60 words
A compound of the formula: wherein R 1 is a group wherein R" is a ligand-analog having at least one common epitope with a ligand so as to he specifically recognizable by a common antibody; R 2 is hydrogen or a group represented by R l ; and R 3 is hydrogen, amino or carboxyl; and biolgically acceptable salts thereof,
Claim 2
Dependent Claim
60 words
A compound of the formula: wherein R 1 is a group wherein R" is a ligand-analog having at least one common epitope with a ligand so as to he specifically recognizable by a common antibody; R 2 is hydrogen or a group represented by R l ; and R 3 is hydrogen, amino or carboxyl; and biolgically acceptable salts thereof,
Claim 3
Dependent Claim
11 words
A compound according to Claim 1 wherein R 2 is hydrogen.
Claim 4
Dependent Claim
11 words
A compound according to Claim 1 wherein R 3 is hydrogen.
Claim 5
Dependent Claim
30 words
A compound according to Claim 2 wherein R" is a ligand-analog having at least one common epitope with a ligand so as to be specifically recognizable by a common antibody.
Claim 6
Dependent Claim
9 words
A compound according to Claim 4 having the formula:
Claim 7
Dependent Claim
9 words
A compound according to Claim 4 having the formula:
Claim 8
Dependent Claim
9 words
A compound according to Claim 4 having the formula:
Claim 9
Dependent Claim
9 words
A compound according to Claim 4 having the formula:
Claim 10
Dependent Claim
9 words
A compound according to Claim 4 having the formula:
Claim 11
Dependent Claim
25 words
10 . A compound of the formula: wherein n is 0 or 1 and R 3 is hydrogen, amino or carboxyl; and acid salts thereof.
Claim 12
Dependent Claim
25 words
10 . A compound of the formula: wherein n is 0 or 1 and R 3 is hydrogen, amino or carboxyl; and acid salts thereof.
Claim 13
Dependent Claim
10 words
A compound according to Claim 10 wherein n is 0.
Claim 14
Independent Claim
108 words
A method for determining ligands in a sample comprising intermixing with said sample a tracer of the formula: wherein R 4 is a group wherein R" is a ligand-analog having at least one common epitope with a ligand so as to be specifically recognizable by a common antibody; R2 is hydrogen or a group represented by Rl ; and R 3 is hydrogen, amino or carboxyl; and biologically acceptable salts thereof; and an antibody capable of specifically recognizing said ligand and said tracer; and then determining the amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Claim 15
Independent Claim
108 words
A method for determining ligands in a sample comprising intermixing with said sample a tracer of the formula: wherein R 4 is a group wherein R" is a ligand-analog having at least one common epitope with a ligand so as to be specifically recognizable by a common antibody; R2 is hydrogen or a group represented by Rl ; and R 3 is hydrogen, amino or carboxyl; and biologically acceptable salts thereof; and an antibody capable of specifically recognizing said ligand and said tracer; and then determining the amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Claim 16
Dependent Claim
12 words
13, A method according to Claim 12 wherein R 2 is hydrogen.
Claim 17
Dependent Claim
19 words
A method according to Claim 13 wherein R" has a molecular weight within a range of 50 to 4000,
Claim 18
Dependent Claim
14 words
A method according to Claim 14 wherein R" is a cortisol-analog of the formula:
Claim 19
Dependent Claim
14 words
A method according to Claim 14 wherein R" is an estriol-analog of the formula;
References Cited (Backward Citations)
FR2414505A1
Cited
FR2500165A1
Cited
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