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EP0104926A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Assay processes and materials therefor
Assignee: Unilever PLC
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Filing: 1983-09-26
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Granted: —
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Expires: 2003-09-26
Abstract
Abstract A process for carrying out a specific binding assay, for the qualitative detection or quantitative or semi-quantitative determination of an analyte which forms a component of a specific binding reaction, using a labelled conjugate form of a derivative or analogue of one of the relevant specific binding partners, said labelled form being capable of emitting delayed luminescence upon photo-excitation, characterised in that the labelled conjugate form of one of the relevant specific binding partners comprises a derivative or analogue of the specific binding partnerwith a derivative of a luminescent substance which shows a delayed light emission upon photoexcitation, the delayed light emission of which is subject to quenching by molecular oxygen, and characterised further in that the assay is carried out by the use of a time-resolved photometric method in the presence of an effective amount of a quench-inhibiting substance able to prevent quenching by molecular oxygen.
Inventors
- 👤 Ahmad Mohammed Sidki
- 👤 David Stuart Smith
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 09:17
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/807; Y10S436/808; Y10S436/815; Y10S436/817; Y10S436/825; Y10S436/826
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
148 words
A process of carrying out a specific binding assay, for the qualitative detection or quantitative or semi-quantitative determination of an analyte which forms a component of a=specific binding reaction, using a labelled conjugate form of a derivative or analogue of one of the relevant specific binding partners, said labelled form being capable of ° emitting delayed luminescence upon photo-excitation, characterised in that the labelled conjugate form of one of the relevant specific binding partners comprises a derivative or analogue of the specific binding partner with a derivative of a luminescent substance which shows a delayed light emission upon photoexcitation, the delayed light emission of which is subject to quenching by molecular oxygen, and characterised further in that the assay is carried out by the use of a time-resolved photometric method in the presence of an effective amount of a quench-inhibiting substance able to prevent quenching by molecular oxygen.
Claim 2
Dependent Claim
148 words
A process of carrying out a specific binding assay, for the qualitative detection or quantitative or semi-quantitative determination of an analyte which forms a component of a=specific binding reaction, using a labelled conjugate form of a derivative or analogue of one of the relevant specific binding partners, said labelled form being capable of ° emitting delayed luminescence upon photo-excitation, characterised in that the labelled conjugate form of one of the relevant specific binding partners comprises a derivative or analogue of the specific binding partner with a derivative of a luminescent substance which shows a delayed light emission upon photoexcitation, the delayed light emission of which is subject to quenching by molecular oxygen, and characterised further in that the assay is carried out by the use of a time-resolved photometric method in the presence of an effective amount of a quench-inhibiting substance able to prevent quenching by molecular oxygen.
Claim 3
Dependent Claim
18 words
A process according to claim 1, characterised in that said quench-inhibiting substance comprises a sulphite, bisulphite or metabisulphite.
Claim 4
Dependent Claim
29 words
A process according to claim 2, characterised in that said quench-inhibiting substance is present in the assay reaction mixture at a concentration of the order of 10 -2 M.
Claim 5
Dependent Claim
52 words
A process according to claim 1, characterised in that said derivative of a luminescent substance is selected from derivatives of halogenated fluorophors, especially brominated and iodinated fluorophors, for example halogenated fluoresceins, such as erythrosin and eosin, and from fluorescent coumarins and flavones, especially 4-methylumbelliferone, and their halogenated, especially brominated and iodinated derivatives.
Claim 6
Dependent Claim
18 words
A process according to claim 4, characterised in that said derivative of a luminescent substance comprises erythrosin (isothiocyanate).
Claim 7
Dependent Claim
21 words
A process according to claim 1, characterised in that the assay utilises an immunoadsorbent and separation of free from bound conjugate.
Claim 8
Dependent Claim
30 words
A process according to claim 1, characterised in that the assay utilises a specific binding partner of the labelled conjugate which quenches the luminescence of the conjugate upon binding therewith.
Claim 9
Dependent Claim
78 words
A process according to claim 1, characterised in that the analyte is selected from drugs including primidone, phenytoin, phenobarbitone, carbamazepine, ethosuximide and valproic acid; antibiotics-including gentamicin, tobramycin, amikacin, netilmicin and sisomycin; antiasthmatic substances including theophylline; cardioactive or antiarrhythmic substances and related substances including quinidine, propranolol, oxprenolol, procainamide, N-acetyl procainamide, lignocaine, digoxin and digitoxin; hormones including thyroid hormones and steroid hormones including oestriol sulphate, progesterone, oestradiol, oestrone-3-glucuronide; other steroids, including bile acids and cholesterol; and proteins and peptide hormones.
Claim 10
Dependent Claim
20 words
A process according to claim 1 and substantially as described in the specification with reference to any of the Examples.
Claim 11
Dependent Claim
93 words
A test kit or reagent set for carrying out a specific binding assay according to claim 1, characterised in that it comprises (i) a labelled conjugate form of one of the relevant specific binding partners comprising a derivative or analogue of the specific binding partner with a derivative of a luminescent substance which shows a delayed light emission upon photoexcitation, the delayed light emission of which is subject to quenching by molecular oxygen, and (ii) an effective amount of a quench-inhibiting substance able to prevent.the oxygen quenching of the luminescence of component (i).
Claim 12
Dependent Claim
29 words
A test kit or reagent set according to claim 10, further characterised by any one of the features set forth in the accompanying specification and claims including the Examples.
References Cited (Backward Citations)
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