EP0103960A2
Expired / Lapsed
EP
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DNA sequences coding for the DR beta-chain locus of the human lymphocyte antigen complex and polypeptides, diagnostic typing processes and products related thereto
Assignee: Biomerieux SA
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Filing: 1983-07-28
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Granted: —
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Expires: 2003-07-28
Abstract
Abstract DNA sequences coding for the DR-β-chain locus of human lymphoctye antigen complex and diagnostic typing processes and products related thereto. DNA sequences that code for the β-chain DR locus are useful in simple and efficient typing processes and products and for expression of polypeptides displaying an immunological or biological activity of the antigens of the HLA-DR β-chains for use in diagnostic, preventive and therapeutic agents.
Inventors
- 👤 Bernard Francois Mach
- 👤 Eric Olivier Long
- 👤 Claire Terese Wake
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-30 04:32
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/68; C12Q1/6876; C12Q1/6881; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C07K14/70503; C07K14/70539
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
96 words
A DNA sequence encoding at least a portion of at least one β-chain antigen of the ELA-DR locus of the human lymphocyte antigen complex, said sequence being selected from the group consisting of (a) the DNA inserts DR-β-A, DR-β-B and DR-P-C, (b) DNA sequences which hybridize under high criterium thereto, and (c) DNA sequences which when expressed code for the polypeptides coded for by the expression of any of the foregoing DNA sequences or inserts, said sequences and inserts encoding a product that displays an immunological or biological activity of a β-chain of the HLA-DR locus.
Claim 2
Dependent Claim
96 words
A DNA sequence encoding at least a portion of at least one β-chain antigen of the ELA-DR locus of the human lymphocyte antigen complex, said sequence being selected from the group consisting of (a) the DNA inserts DR-β-A, DR-β-B and DR-P-C, (b) DNA sequences which hybridize under high criterium thereto, and (c) DNA sequences which when expressed code for the polypeptides coded for by the expression of any of the foregoing DNA sequences or inserts, said sequences and inserts encoding a product that displays an immunological or biological activity of a β-chain of the HLA-DR locus.
Claim 3
Dependent Claim
83 words
The DNA sequence of claim 1, wherein said sequence (b) which hybridizes to said sequence (a) is selected from the group consisting of: (d) the DNA insert of DR-p-D, (e) DNA sequences which hybridize under high criterium thereto; (f) DNA sequences which when expressed code for the polypeptides coded for by the expression of any of the foregoing DNA sequences or inserts, said sequences and inserts encoding a product that displays an immunological or biological activity of a β-chain of the HLA-DR locus.
Claim 4
Dependent Claim
9 words
A DNA sequence selected from the group consisting of:
Claim 5
Dependent Claim
9 words
A DNA sequence selected from the group consisting of:
Claim 6
Dependent Claim
9 words
A DNA sequence selected from the group consisting of:
Claim 7
Dependent Claim
9 words
A DNA sequence selected from the group consisting of:
Claim 8
Dependent Claim
9 words
A DNA sequence selected from the group consisting of:
Claim 9
Dependent Claim
9 words
A DNA sequence selected from the group consisting of:
Claim 10
Dependent Claim
14 words
A DNA sequence selected from the group consisting of TGGAGCAGGTTAAACATGA, TCCTGGACAGATACTTC TA, and GGGCCGCGGTGGACACCTA.
Claim 11
Dependent Claim
14 words
A DNA sequence selected from the group consisting of TGGAGCAGGTTAAACATGA, TCCTGGACAGATACTTC TA, and GGGCCGCGGTGGACACCTA.
Claim 12
Dependent Claim
45 words
A DNA sequence selected from the group consisting of DNA insert DR-β-C, the expressed portion of the DNA insert of DR-β-C, and fragments of either member of this group that encode products displaying an immunological or biological activity of a β-chain of the ELA-DR locus.
Claim 13
Dependent Claim
45 words
A DNA sequence selected from the group consisting of DNA insert DR-β-C, the expressed portion of the DNA insert of DR-β-C, and fragments of either member of this group that encode products displaying an immunological or biological activity of a β-chain of the ELA-DR locus.
Claim 14
Dependent Claim
45 words
A DNA sequence selected from the group consisting of DNA insert DR-β-D, the expressed portion of the DNA insert of DR-β-D, and fragments of either member of this group that encode products displaying an immunological or biological activity of a β-chain of the HLA-DR locus.
Claim 15
Dependent Claim
45 words
A DNA sequence selected from the group consisting of DNA insert DR-β-D, the expressed portion of the DNA insert of DR-β-D, and fragments of either member of this group that encode products displaying an immunological or biological activity of a β-chain of the HLA-DR locus.
Claim 16
Dependent Claim
22 words
A recombinant DNA molecule comprising a DNA sequence selected from the DNA sequences of any one of claims 1-3, 5 and 7-8.
Claim 17
Dependent Claim
24 words
The recombinant DNA molecule of claim 9 wherein the DNA sequence is operatively linked to an expression control sequence in said recombinant DNA molecule.
Claim 18
Dependent Claim
41 words
A polypeptide displaying an immunological or biological activity of at least one p-chain antigen of the HLA-DR locus of the human lymphocyte antigen complex produced by a process of culturing a host transformed with the recombinant DNA molecule of claim 10.
Claim 19
Dependent Claim
12 words
A polypeptide selected from the group consisting of polypeptides of the formula:
Claim 20
Dependent Claim
12 words
A polypeptide selected from the group consisting of polypeptides of the formula:
Claim 21
Dependent Claim
12 words
A polypeptide selected from the group consisting of polypeptides of the formula:
Claim 22
Dependent Claim
12 words
A polypeptide selected from the group consisting of polypeptides of the formula:
Claim 23
Dependent Claim
44 words
A process for producing a DNA sequence encoding at least one β-chain antigen of the HLA-DR locus of the human lymphocyte antigen complex comprising the steps of culturing a host transformed with a recombinant DNA molecule of claim 9, and isolating said DNA sequence.
Claim 24
Dependent Claim
48 words
A process for producing a polypeptide displaying an immunological or biological activity of at least one β-chain antigen of the HLA-DR locus of the human lymphocyte antigen complex comprising the steps of culturing a host transformed with a recombinant DNA molecule of claim 10 and collecting the polypeptide.
Claim 25
Dependent Claim
50 words
An HLA-DR typing process comprising the steps of restricting DNA isolated from the individual to be typed with at least one restriction endonuclease; size fractionating the restricted DNA; hybridizing the size-fractionated DNA to a DNA sequence of any one of claims 1 to 8 and detecting the areas of hybridization.
Claim 26
Dependent Claim
27 words
The process of claim 16, wherein a 32 P-labelled DNA sequence is employed for hybridization and its radioactive label is used for detecting the areas of hybridization.
Claim 27
Dependent Claim
43 words
An HLA-DR typing process comprising the steps of restricting DNA isolated from the individual to be typed with at least one restriction endonuclease; size fractionating the restricted D NA ; hybridizing the sizefractionated DNA to a 19-mer selected from the group consisting of
Claim 28
Dependent Claim
43 words
An HLA-DR typing process comprising the steps of restricting DNA isolated from the individual to be typed with at least one restriction endonuclease; size fractionating the restricted D NA ; hybridizing the sizefractionated DNA to a 19-mer selected from the group consisting of
Claim 31
Dependent Claim
17 words
The typing process of claim 18 wherein the hybridization control is a 19-mer of the formula GCTTCGACAGCGACGTGGG.
Claim 32
Dependent Claim
17 words
An HLA-DR typing kit characterized by a DNA sequence of any one of claims 1 to 8.
Claim 33
Dependent Claim
21 words
In an HLA-DR typing process, the improvement comprising employing a polypeptide of claim 11-13 or an antibody raised against those polypeptides.
Claim 34
Dependent Claim
21 words
In an ELA-DR typing kit, the improvement comprising employing a polypeptide of claim 11-13 or an antibody raised against those polypeptides.
References Cited (Backward Citations)
No citation records indexed.
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