EP0103901A2
Expired / Lapsed
EP
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Multilayer analytical element
Assignee: Fujifilm Holdings Corp
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Filing: 1983-09-21
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Granted: —
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Expires: 2003-09-21
Abstract
Abstract A multilayer analytical element comprising a water-impermeable, light-transmissive support, a reagent layer containing a color forming reagent composition for detection of hydrogen peroxide including at least combination of a chromogen and a coupler (a color indicator composition), and peroxidase, which produces a detectable change in the presence of peroxidase and hydrogen peroxide, and a porous spreading layer, which are superposed in this order, in which said chromogen is a 1,2,3,-tri-substituted compound of 4-amino-3-pyrazoiin-5-one having solubility in water at 25°C of lower than that of 4-amino-2, 3-dimethyl-1-phenyl-3-pyrazolin-5-one.
Inventors
- 👤 Fuminori Arai
- 👤 Masao Kitajima
- 👤 Yoshio Inagaki
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-28 17:43
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; G; G01; G01N; G01N31/00; G01N31/22; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; C12Q2326/00; C12Q2326/90; C12Q2326/96
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
85 words
A multilayer analytical element comprising a water-impermeable, light-transmissive support, a reagent layer containing a color forming reagent composition for detection of hydrogen peroxide including at least a combination of a chromogen and a coupler, and peroxidase, which produces a detectable change in the presence of peroxidase and hydrogen peroxide , and a porous spreading layer, which are superposed in this order, in which said chromogen is a 1,2,3-tri-substituted compound of 4-amino-3-pyrazolin-5-one having solubility in water at 25 0 C of lower than that of 4-amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one.
Claim 2
Dependent Claim
85 words
A multilayer analytical element comprising a water-impermeable, light-transmissive support, a reagent layer containing a color forming reagent composition for detection of hydrogen peroxide including at least a combination of a chromogen and a coupler, and peroxidase, which produces a detectable change in the presence of peroxidase and hydrogen peroxide , and a porous spreading layer, which are superposed in this order, in which said chromogen is a 1,2,3-tri-substituted compound of 4-amino-3-pyrazolin-5-one having solubility in water at 25 0 C of lower than that of 4-amino-2,3-dimethyl-1-phenyl-3-pyrazolin-5-one.
Claim 3
Dependent Claim
41 words
The multilayer analytical element as claimed in claim 1, in which said coupler is a compound capable of forming a dye together with the 1,2,3-tri-substituted compound of 4-amino-3-pyrazolin-5-one through oxidative coupling reaction in the presence of peroxidase and hydrogen peroxide. -
Claim 4
Dependent Claim
26 words
The multilayer analytical element as claimed in claim 1 or 2, in which said reagent layer contains said 1,2,3-tri-substituted compound of 4-amino-3-pyrazolin-5-one, said coupler and peroxidase.
Claim 5
Dependent Claim
27 words
The multilayer analytical element as claimed in any one of claims 1 through 3, in which oxidase is contained in said reagent layer or any other layer.
Claim 6
Dependent Claim
34 words
The multilayer analytical element as claimed in claim 4, in which said oxidase is selected from the group consisting of glucose oxidase, cholesterol oxidase, pyruvate oxidase, uricase, ascorbate oxidase, lactate oxidase, and glycerol oxidase.
Claim 7
Dependent Claim
16 words
The multilayer analytical element as claimed in claim 4, in which said oxidase is glucose oxidase.
Claim 8
Dependent Claim
16 words
The multilayer analytical element as claimed in claim 4, in which said oxidase is cholesterol oxidase.
References Cited (Backward Citations)
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