Process for producing substantially pure dermatan sulfate and heparan sulfate glycosaminoglycans, and pharmaceutical use thereof

Claim 1

Process for producing substantially pure dermatan sulfate and heparan sulfate, starting from a mixture of mucopolysaccharides in aqueous solution which contains them, characterized by operations of: a) introducing in said starting solution a water soluble ionizing salt'consisting of ammonium or potassium as the cation part, up to a concentration of 70 - 90% of the saturation level of said salt at 25°C, so as to produce at room temperature a first precipitate containing dermatan sulfate in a substantially pure state; b) separating said precipitate from the solution and recovering the substantially pure dermatan sulfate as first product; c) cooling the solution removed from the precipitate to approximately O°C in order to produce a second precipitate at said temperature containing chondroitin sulfate A; d) removing said second precipitate from the solution; e) separating from the residual solution the salt introduced in operation (a) so as to prepare a desalinified solution; f) removing the water from said desalinified solution so as to prepare a solid containing heparan sulfate contaminated with other glycosaminiglycanes; g) subjecting said solid to enzymatic treatment with pndroitinase ABC, so as to eliminate said impurities and recover the heparan sulfate substantially pure as second product.