EP0091258A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method for stabilizing tumor necrosis factor and a stable aqueous solution or powder containing said factor
Assignee: Asahi Kasei Corp
•
Filing: 1983-03-28
•
Granted: —
•
Expires: 2003-03-28
Abstract
Abstract A method for stabilizing a Tumor Necrosis Factor (TNF), which comprises adding at least one member selected from the group consisting of an albumin, a geiatin, a globulin, a protamine and a salt of protamine to an aqueous solution or powder containing TNF, and a stable aqueous solution or powder which contains TNF and an effective amount of such a protein. The aqueous solution or powder containing TNF can be stored for a prolonged period of time without losing its activity, and is stable on freezing, thawing, lyophilization or the like.
Inventors
- 👤 Hajimu Sakamoto
- 👤 Takao Kiyota
- 👤 Hiroshi Hayashi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 06:55
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/08; A61K38/00; A61K38/16; A61K38/17; A61K38/19; A61K38/191; A61K47/00; A61K47/30; A61K47/42
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
44 words
A method for stabilizing Tumor Necrosis Factor, which comprises adding to an aqueous solution or powder containing Tumor Necrosis Factor an effective amount of at least one stabilizing agent selected from an albumin, a gelatin, a globulin, a protamine and a salt of protamine.
Claim 2
Independent Claim
44 words
A method for stabilizing Tumor Necrosis Factor, which comprises adding to an aqueous solution or powder containing Tumor Necrosis Factor an effective amount of at least one stabilizing agent selected from an albumin, a gelatin, a globulin, a protamine and a salt of protamine.
Claim 3
Dependent Claim
14 words
A method according to claim 1, wherein said stabilizing agent is human serum albumin.
Claim 4
Dependent Claim
16 words
A method according to claim 1, wherein said stabilizing agent is a water-soluble partially hydrolyzed gelatin.
Claim 5
Dependent Claim
18 words
A method according to claim 1, wherein said stabilizing agent is human gamma globulin or a derivative thereof.
Claim 6
Independent Claim
50 words
A method according to any one of claims 1 to 4, wherein said stabilizing agent is added in an amount of 10 µg to 50 mg per ml of the aqueous solution containing Tumor Necrosis Factor, said aqueous solution having a TNF activity of 10 2 to 10 9 units/ml.=
Claim 7
Dependent Claim
20 words
A method according to claim 5, wherein the amount of stabilizing agent is 100 pg to 10 mg per ml.
Claim 8
Independent Claim
27 words
A method according to any one of claims 1 to 6, which further comprises subjecting to lyophilization the resulting aqueous solution having said stabilizing agent added thereto.
Claim 9
Dependent Claim
31 words
A stable aqueous solution or powder which contains Tumor Necrosis Factor and an effective amount of at least one stabilizing agent as defined in any one of claims 1 to 4.
Claim 10
Dependent Claim
86 words
A stable aqueous solution or powder according to claim 8, wherein said stabilizing agent is contained in an amount of 10 pg to 50 mg per ml of the aqueous solution itself containing Tumor Necrosis Factor, said aqueous solution having a TNF activity of 10 to 10 units/ml, or is contained in an amount of 10 pg to 50 mg per ml of an aqueous solution having a TNF activity of 10 2 to 10 9 units/ml obtained by dissolving the powder containing Tumor Necrosis Factor.
Claim 11
Dependent Claim
46 words
A stable aqueous solution or powder according to claim 9 wherein the amount of stabilizing agent is 100 µg to 10 mg per ml of the aqueous solution itself containing Tumor Necrosis Factor or an aqueous solution obtained by dissolving the powder containing Tumor Necrosis Factor.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.