Alpha-interferon Gx-1

Claim 2

An isolated, intact human alpha-interferon Gx-1 gene.

Claim 3

The alpha-interferon Gx-1 gene of claim 1, comprising the following deoxyribonucleotide sequence: wherein the 5' to 3' strand, beginning with the amino terminus and the amino acids for which each triplet codes are shown, and wherein A is deoxyadenyl T is thymidyl G is deoxyguanyl C is deoxycytosyl X is. A, T, C or G Y is T or C When Y is C, Z is A, T, C or G When Y is T, Z is A or G H is A, T or C Q is T or A When Q is T, R is C and S is A, T, C or G When Q is A, R is G and S is.T or C M is A or G L is A or C When L is A, N is A or G When L is C, N is A, T, C or G GLY is glycine ALA is alanine VAL is valine LEU is leucine ILE is isoleucine SER is serine THR is threonine PHE is phenylalanine TYR is tyrosine TRP is tryptophan CYS is cysteine MET is methionine ASP is aspartic acid GLU is glutamic acid LYS is lysine ARG is arginine HIS is histidine PRO is proline GLN is glutamine ASN is asparagine

Claim 4

The alpha-interferon Gx-1 gene of claim 2, comprising the following deoxyribonucleotide sequence:

Claim 5

The alpha-interferon Gx-1 gene of claim 2, comprising the following deoxyribonucleotide sequence: MET MET

Claim 6

The alpha-interferon Gx-1 gene of claims 2, comprising the following deoxyribonucleotide sequence:

Claim 7

A plasmid having the capability of replication in a unicellular procaryotic or eucaryotic organism, comprising a deoxynucleotide sequence coding for human alpha-interferon Gx-1.

Claim 8

A plasmid having the capability of replication in a unicellular procaryotic or eucaryotic organism, comprising a deoxynucleotide sequence coding for human alpha-interferon Gx-1.

Claim 9

The plasmid of claim 6, further comprising promoter, operator and translation initiation sequences which are capable of directing transcription and the translation of the deoxyribonucleotide sequence coding for human alpha-interferon Gx-1 in a procaryotic organism.

Claim 10

A plasmid having the capability of replication in a procaryotic organism, comprising the alpha-interferon Gx-1 gene of claim 3, 4 or 5.

Claim 11

The plasmid of claim 8, further comprising promoter, operator and translation initiation sequences which are capable of directing transcription and translation of the deoxyribonucleotide sequence coding for human alpha-interferon Gx-1 in a procaryotic organism.

Claim 12

The plasmid of claim 8, wherein said procaryotic organism is of the genus Escherichia.

Claim 13

ll. The plasmid of claim 9, wherein the promoter, operator and translation initiation sequences are capable of directing transcription and translation in a bacterium of the genus Escherichia

Claim 14

The plasmid of claim 11, wherein the promoter, operator and translation initiation sequences are derived from the lac or trp operon of Escherichia coli

Claim 15

A microorganism transformed by the plasmid of claim 6 or 7.

Claim 16

A microroganism transformed by the plasmid of claim 8.

Claim 17

The microorganism of claim 14, of the genus Escherichia.

Claim 18

The microorganism of claim 15, of the species coli.

Claim 19

A microorganism of the genus and species of Escherichia coli designated as A3-26 and deposited with the American Type Culture Collection as ATCC No. 39063.

Claim 20

A microorganism of the genus and species of Escherichia coli designated as A3-26 and deposited with the American Type Culture Collection as ATCC No. 39063.

Claim 23

The method of claim 18, wherein the procaryotic organism is Escherichia coli.

Claim 24

The method of claim 19, wherein said procaryotic organism is strain A3-26.

Claim 25

Alpha-interferon Gx-1.

Claim 26

Alpha-interferon Gx-1.

Claim 27

Alpha-interferon Gx-1, comprising the amino acid sequence shown in claim 2.

Claim 28

Alpha-interferon Gx-1, comprising the amino acid sequence shown in claim 3.

Claim 29

Alpha-interferon Gx-1, comprising the amino acid sequence shown in claim 4.