EP0089666A2
Expired / Lapsed
EP
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Human growth hormone variants
Assignee: Genentech Inc
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Filing: 1983-03-21
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Granted: —
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Expires: 2003-03-21
Abstract
Abstract Variants of human growth hormone, differing therefrom in structure but not essential activity, from a basis of the present invention and disclosure. In addition, the present invention involves a novel approach for isolating cDNA for a protein of unknown identity or detectability in native state.
Inventors
- 👤 Peter H. Seeburg
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-06 08:23
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/102; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/12
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
14 words
A human growth hormone variant protein differing in structure from natural human growth hormone.
Claim 2
Dependent Claim
14 words
A human growth hormone variant protein differing in structure from natural human growth hormone.
Claim 3
Dependent Claim
17 words
The human growth hormone variant protein having the amino acid sequence set forth in Figure 3 hereof.
Claim 4
Dependent Claim
17 words
The human growth hormone variant protein having the amino acid sequence set forth in Figure 3 hereof.
Claim 5
Dependent Claim
14 words
The human growth hormone variant protein according to claim 2 including the presequence thereof.
Claim 6
Independent Claim
75 words
A method of obtaining cDNA encoding a desired polypeptide which comprises the steps of: a) probing genomic DNA to obtain genomic sections containing gene for said polypeptide; b) incorporating the genomic sections of step a) into host cells and permitting transcription of same into corresponding mRNA, and c) isolating the cDNA for said polypeptide by creating a cDNA bank from the mRNA of step b) and probing for the requisite DNA sequence of said polypeptide.
Claim 7
Independent Claim
75 words
A method of obtaining cDNA encoding a desired polypeptide which comprises the steps of: a) probing genomic DNA to obtain genomic sections containing gene for said polypeptide; b) incorporating the genomic sections of step a) into host cells and permitting transcription of same into corresponding mRNA, and c) isolating the cDNA for said polypeptide by creating a cDNA bank from the mRNA of step b) and probing for the requisite DNA sequence of said polypeptide.
Claim 8
Dependent Claim
10 words
The method of claim 4 useful for producing HGH-V cDNA.
Claim 9
Dependent Claim
20 words
The method according to claim 4 wherein the genomic sections of step a) are integrated into permissive Cos cell vectors.
Claim 10
Dependent Claim
19 words
The DNA sequence encoding a human growth hormone variant protein having the sequence set forth in Figure 3 hereof.
Claim 11
Dependent Claim
19 words
The DNA sequence encoding a human growth hormone variant protein having the sequence set forth in Figure 3 hereof.
Claim 12
Dependent Claim
11 words
The DNA sequence according to claim 7 including the presequence thereof.
Claim 13
Dependent Claim
11 words
A replicable cloning vehicle containing the DNA sequence of claim 7.
Claim 14
Dependent Claim
26 words
An expression vector comprising a DNA sequence according to claim 7 operably linked to expression effecting DNA sequence and flanked by translational start and stop signals.
Claim 15
Dependent Claim
12 words
A viable cell culture transformed with the expression vector of claim 10.
Claim 16
Dependent Claim
15 words
A cell culture capable of producing the human growth hormone variant according to claim 2.
References Cited (Backward Citations)
No citation records indexed.
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