EP0088636A2
Expired / Lapsed
EP
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Quantitative analysis apparatus and method
Assignee: BIO-METRICS SYSTEMS Inc
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Filing: 1983-03-09
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Granted: —
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Expires: 2003-03-09
Abstract
Abstract A method and apparatus for the quantitative determination of an analyte in a liquid employs a liquid-permeable solid medium defining a liquid flow path. The medium includes a number of reactant-containing reaction zones spaced apart along the flow path and in which reaction occurs with the analyte or an analyte derivative (e.g., a labelled analyte) to result in the formation of a predetermined product. Detector means are employed to detect analyte, analyte derivative, reactant or predetermined product in the reaction zones, the number of such zones in which such detection occurs indicating the amount of analyte in the liquid.
Inventors
- 👤 Melvin J. Swanson
- 👤 Patrick E. Guire
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 16:54
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; G01N33/54389; G01N33/54391; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S435/968; Y10S435/97; Y10S436/00; Y10S436/805; Y10S436/807; Y10S436/81; Y10S436/815
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
114 words
Apparatus for the quantitative analysis of an analyte in a fluid, characterized in that it comprises a fluid-permeable solid medium defining a path for fluid flow and having a predetermined number of successive, spaced reaction zones (16, 16.1, 16.2) in the path of flow, the reaction zones having immobilized therein predetermined quantities of a reactant capable of reacting with the analyte or with an analyte derivative to result in the formation of a predetermined product; whereby the analyte, the analyte derivative, the reactant or the predetermined product in the reaction zones can be detected, and the number of reaction zones in which such detection occurs indicating quantitatively the amount of analyte in said fluid.
Claim 2
Dependent Claim
114 words
Apparatus for the quantitative analysis of an analyte in a fluid, characterized in that it comprises a fluid-permeable solid medium defining a path for fluid flow and having a predetermined number of successive, spaced reaction zones (16, 16.1, 16.2) in the path of flow, the reaction zones having immobilized therein predetermined quantities of a reactant capable of reacting with the analyte or with an analyte derivative to result in the formation of a predetermined product; whereby the analyte, the analyte derivative, the reactant or the predetermined product in the reaction zones can be detected, and the number of reaction zones in which such detection occurs indicating quantitatively the amount of analyte in said fluid.
Claim 3
Dependent Claim
28 words
An apparatus according to Claim 1, characterized in that said fluid-permeable solid medium includes spacer sections (18, 18.1, 18.2) in flow communication with but separating said reaction zones.
Claim 4
Dependent Claim
31 words
An apparatus according to Claim 1 or 2, characterized in that the fluid-permeable medium in the reaction zones has immobilized therein an antibody specific to the analyte to be quantitatively analyzed.
Claim 5
Dependent Claim
33 words
An apparatus according to Claim 1, 2 or 3 characterized in that it includes detection means for detecting, in the reaction zones, the analyte, the analyte derivative, the reactant, or the predetermined product.
Claim 6
Independent Claim
43 words
An apparatus according to any of the preceding claims, characterized in that there is included in the reaction zones or spacer layers means for suppressing therein the detection of trace amounts of the analyte, the analyte derivative, the reactant or the predetermined product.
Claim 7
Dependent Claim
51 words
An apparatus according to Claim 3, characterized in that said reaction zones are adapted to change color upon contact therein with an analyte derivative that is an analyte-enzyme conjugate, the reaction zones having immobilized therein an enzyme, one of said enzymes being capable of generating substrate for the other said enzyme.
Claim 8
Dependent Claim
42 words
An apparatus according to Claim 6, characterized in that said analyte-enzyme conjugate is contained in a layer upstream from a reaction zone, whereby flow of the analyte in the fluid carrier through said layer carries the analyte conjugate into said reaction zone.
Claim 9
Independent Claim
119 words
A method for the quantitative analysis of an analyte in a carrier liquid, characterized by the steps of: providing a fluid-permeable solid medium defining a flow path and having a predetermined number of successive, spaced reaction zones in the path of flow, said reaction zones having immobilized therein a reactant reactive with the analyte or an analyte derivative or both to result in the formation of a predetermined product; flowing said fluid along the flow path and sequentially though the spaced reaction zones; and detecting the presence of analyte, analyte derivative, reactant or predetermined product in the reaction zones; the amount of analyte in the fluid being a function of the number of zones in which such detection occurs.
Claim 10
Independent Claim
119 words
A method for the quantitative analysis of an analyte in a carrier liquid, characterized by the steps of: providing a fluid-permeable solid medium defining a flow path and having a predetermined number of successive, spaced reaction zones in the path of flow, said reaction zones having immobilized therein a reactant reactive with the analyte or an analyte derivative or both to result in the formation of a predetermined product; flowing said fluid along the flow path and sequentially though the spaced reaction zones; and detecting the presence of analyte, analyte derivative, reactant or predetermined product in the reaction zones; the amount of analyte in the fluid being a function of the number of zones in which such detection occurs.
Claim 11
Dependent Claim
23 words
A method according to Claim 8 , characterized in that said analyte and said reactant are ones of a specific ligand-antiligand binding pair.
Claim 12
Dependent Claim
18 words
A method according to Claim 9, characterized in that said reactant includes an antibody specific to said reactant.
Claim 13
Dependent Claim
34 words
A method according to Claim 8, 9 or 10, characterized in that reaction between said analyte and reactant renders the analyte, analyte derivative, reactant or predetermined reaction product non- detectable during said detection step.
Claim 14
Independent Claim
106 words
A method for the quantitative determination of an analyte in a fluid containing a known quantity of a labeled analyte, characterized by comprising the steps of: providing a fluid-permeable solid medium defining a fluid flow path and having immobilized therein a reactant reactive with the labeled analyte in competition with the analyte to result in the formation of a predetermined product; flowing said fluid along the flow path; and detecting the presence of the labeled analyte or the predetermined product along the flow path; the length of the flow path in which such detection occurs being a function of the amount of analyte in the fluid.
Claim 15
Independent Claim
106 words
A method for the quantitative determination of an analyte in a fluid containing a known quantity of a labeled analyte, characterized by comprising the steps of: providing a fluid-permeable solid medium defining a fluid flow path and having immobilized therein a reactant reactive with the labeled analyte in competition with the analyte to result in the formation of a predetermined product; flowing said fluid along the flow path; and detecting the presence of the labeled analyte or the predetermined product along the flow path; the length of the flow path in which such detection occurs being a function of the amount of analyte in the fluid.
Claim 16
Dependent Claim
20 words
A method according to Claim 12, characterized in that the analyte is penicillin and the reactant contains an anti-penicillin antibody.
Claim 17
Dependent Claim
22 words
A method according to Claim 8, characterized in that only a single fluid containing an analyte is flowed in said flow path.
Claim 18
Dependent Claim
28 words
A method according to Claim 8, characterized in that it includes the step of suppressing the detection of trace amounts of analyte, analyte derivative, reactant or predetermined product.
References Cited (Backward Citations)
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