Method for the immuno-chemical detection of substances

Abstract

Abstract Essentially three incubations take place in the method, interrupted by cleaning processes that are not represented. A reagent, e.g. an antibody (Ab 1), which is bound to a solid phase, is subjected to a first incubation (I) together with the sample containing an antigen (Ag) which is sought. The unbound sample parts are removed, and the solid phase (1) is mixed with a peroxidase solution. The latter contains peroxidase (POD) which is bound to an antibody (Ab 2) which is likewise specific to the antigen (Ag) to be found but generally different from the antibody (Ab 1). During this second incubation (II), peroxidase (POD) is bound indirectly and in accordance with the antigen (Ag) to be determined via the latter to the solid phase (1). Finally, free or bound peroxidase (POD) is used in a third incubation (III) in order in the presence of hydrogen peroxide H2O2 to liberate from a fluorinated phenolic compound R-F a corresponding quantity of fluoride ions F<->, which are determined in turn electrochemically in an ion-selective fashion. <IMAGE>

Inventors

• 👤 Rainer Lauterburg

Bibliographic Details