EP0083286A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Modified vaccinia virus and methods for making and using the same
Assignee: Health Research Inc
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Filing: 1982-12-23
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Granted: —
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Expires: 2002-12-23
Abstract
Abstract What are disclosed are methods for modifying the genome of vaccinia virus to produce vaccinia mutants, particularly by the introduction into the vaccinia genome of exogenous DNA; modified vaccinia prepared by such methods: certain DNA sequences and unmodified and genetically modified microorganisms involved as intermediates in such methods; and methods for infecting cells and host animals with such vaccinia mutants to provoke the amplification of exogenous DNA and proteins encoded by the exogenous DNA, including antigenic proteins, by said cells and host animals.
Inventors
- 👤 Enzo Paoletti
- 👤 Dennis Panicali
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-07 08:56
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/86; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; C12N2710/00; C12N2710/00011; C12N2710/16011; C12N2710/16611; C12N2710/16622; C12N2710/24011; C12N2710/24111; C12N2710/24141; C12N2710/24143; C12N2760/00; C12N2760/00011; C12N2760/16011; C12N2760/16111; C12N2760/16122
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
14 words
A vaccinia virus modified by the presence of exogenous DNA in the vaccinia genome.
Claim 2
Dependent Claim
14 words
A vaccinia virus modified by the presence of exogenous DNA in the vaccinia genome.
Claim 3
Dependent Claim
29 words
A vaccinia virus as in Claim 1 which is vaccinia virus VP-2, VP-3, VP-4, VP-5, VP-6, VP-7, VP-8, VP-9, VP-10, VP-11, VP-12, VP-13, VP-14, VP-16, VP-17, VP-18, or VP-22.
Claim 4
Dependent Claim
20 words
A biologically pure culture of a vaccinia virus selected from the group consisting of VTK-79 and VTK - 79 L.
Claim 5
Dependent Claim
20 words
A biologically pure culture of a vaccinia virus selected from the group consisting of VTK-79 and VTK - 79 L.
Claim 6
Independent Claim
30 words
The method of replicating DNA in a eukaryotic cell by infecting said cell with a vaccinia virus modified to contain said DNA, said DNA being exogenous to said vaccinia virus.
Claim 7
Independent Claim
30 words
The method of replicating DNA in a eukaryotic cell by infecting said cell with a vaccinia virus modified to contain said DNA, said DNA being exogenous to said vaccinia virus.
Claim 8
Independent Claim
47 words
The method of immunizing a host animal, susceptible to vaccinia virus, by inducing said animal to develop antibodies against an antigen, which method comprises inoculating said host animal with a vaccinia virus having, within the vaccinia genome, DNA exogenous to said genome and coding for said antigen.
Claim 9
Independent Claim
47 words
The method of immunizing a host animal, susceptible to vaccinia virus, by inducing said animal to develop antibodies against an antigen, which method comprises inoculating said host animal with a vaccinia virus having, within the vaccinia genome, DNA exogenous to said genome and coding for said antigen.
Claim 10
Dependent Claim
14 words
A method as in Claim 5 wherein said vaccinia virus is vaccinia virus VP-9.
Claim 11
Independent Claim
74 words
A method for the in vivo recombination of a vaccinia virus and donor DNA which comprises contacting a cell monolayer with a cell-compatible medium containing said vaccinia virus and with a cell-compatible medium containing said donor DNA, said donor DNA comprising DNA to be . introduced into the vaccinia genome present in a DNA segment otherwise co-linear with DNA present in the genome of said vaccinia virus in a non-essential region of said genome.
Claim 12
Independent Claim
74 words
A method for the in vivo recombination of a vaccinia virus and donor DNA which comprises contacting a cell monolayer with a cell-compatible medium containing said vaccinia virus and with a cell-compatible medium containing said donor DNA, said donor DNA comprising DNA to be . introduced into the vaccinia genome present in a DNA segment otherwise co-linear with DNA present in the genome of said vaccinia virus in a non-essential region of said genome.
Claim 13
Dependent Claim
20 words
A method as in Claim 7 wherein said DNA present in the genome of said vaccinia virus is vaccinia DNA.
Claim 14
Dependent Claim
23 words
A method as in Claim 7 wherein said DNA present in the genome of said vaccinia virus is DNA exogenous to vaccinia virus.
Claim 15
Dependent Claim
19 words
A method as in Claim 9 wherein said DNA exogenous to vaccinia virus is the pBR 322 DNA sequence.
References Cited (Backward Citations)
No citation records indexed.
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