EP0077689A2
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EP
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Method of gene manipulation using an eukaryotic cell as the host
Assignee: SUNTORY KK
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Filing: 1982-10-19
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Granted: —
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Expires: 2002-10-19
Abstract
Abstract A cell of an eukaryote such as a yeast is transformed with a gene in which the untranslatable 3' end portion of a structural gene is added downstream from an exogenous gene. The resulted transformant reveals far higher level of expression with the exogenous gene than the case of being not added with the 3' end portion.
Inventors
- 👤 Isamu Takano
- 👤 Teiichi Ono
- 👤 Shoji Tanaka
- 👤 Takehiro Oshima
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 17:03
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C07; C07K; C07K14/00; C07K14/435; C07K14/665
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
42 words
A method of gene manipulation using an eukaryotic cell as the host which comprises transforming an eukaryotic cell with a gene comprising the untranslatable 3' end portion of a structural gene as added downstream from an exogenous gene desired to be expressed.
Claim 2
Independent Claim
42 words
A method of gene manipulation using an eukaryotic cell as the host which comprises transforming an eukaryotic cell with a gene comprising the untranslatable 3' end portion of a structural gene as added downstream from an exogenous gene desired to be expressed.
Claim 3
Dependent Claim
16 words
A method according to claim 1 wherein the eukaryotic cell is the cell of a yeast.
Claim 4
Dependent Claim
19 words
A method according to claim 1 wherein the eukaryotic cell is the cell of a microorganism belonging to Sacchromyces.
Claim 5
Dependent Claim
28 words
A method according to claim 1 wherein the exogenous gene is a chemically synthesized DNA or a cDNA obtained from a mRNA by the use of reverse transcriptase.
Claim 6
Dependent Claim
22 words
A method according to claim 1 wherein the exogenous . gene is somatostatin, alpha-neoendorphin, calcitonin, insulin, an interferon or a growth hormone.
Claim 7
Dependent Claim
48 words
A method according to claim 1 wherein a yeast is transformed with a gene prepared by joining alpha-neoendorphin with a shuttle vector and adding the region corresponding to the untranslatable 3' end portion lost in the course of construction of the shuttle vector downstream from the alpha-neoendorphin gene.
Claim 8
Dependent Claim
34 words
A cell derived by the transformation of a eukaryotic cell with a gene comprising the untranslatable 3' end portion of a structural gene as added downstream of an exogenous gene desired to be expressed.
Claim 9
Dependent Claim
34 words
A cell derived by the transformation of a eukaryotic cell with a gene comprising the untranslatable 3' end portion of a structural gene as added downstream of an exogenous gene desired to be expressed.
Claim 10
Dependent Claim
23 words
A product derived from.a cell as claimed in Claim 7, or a method as claimed in any one of Claims 1 to 6.
References Cited (Backward Citations)
No citation records indexed.
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