EP0075293A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Method for inhibiting beta-D-galactosidase
Assignee: Ajinomoto Co Inc
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Filing: 1982-09-17
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Granted: —
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Expires: 2002-09-17
Abstract
Abstract beta -D-galactosidase catalyzed reactions can be terminated by inhibiting beta -D-galalctosidase in a method in which beta -D-galactosidase is brought in contact with a chelating agent at an alkaline pH range other than the optimal pH range for beta -D-galactosidase.
Inventors
- 👤 Tomoharu Takenouchi
- 👤 Akira Kamimura
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-08 01:45
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/99; C12Q; C12Q1/00; C12Q1/34; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/914; G01N2333/924
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
32 words
A method for inhibiting B-D-galactosidase, characterized by bringing B-D-galactosidase and a chelating agent in contact with each other at an alkaline pH range other than the optimal pH range for β-D-galactosidase activity.
Claim 2
Independent Claim
32 words
A method for inhibiting B-D-galactosidase, characterized by bringing B-D-galactosidase and a chelating agent in contact with each other at an alkaline pH range other than the optimal pH range for β-D-galactosidase activity.
Claim 3
Dependent Claim
28 words
A method according to claim 1 wherein the chelating agent has 2 or more functional groups coordinating a metal ion by means of oxygen, nitrogen and/or sulfur atoms.
Claim 4
Dependent Claim
34 words
A method according to claim 2 wherein the chelating agent is selected from ethylenediaminetetraacetic acid, ethylenediamine, oxalic acid, oxines, amino'acids, acetylacetone, pyrophosphoric acid, hydroxylamine, chelating polymers, metal adsorbing polymers and salts and mixtures thereof.
Claim 5
Dependent Claim
17 words
A method according to claim 3 wherein the chelating agent is ethylenediaminetetraacetic acid or a salt thereof.
Claim 6
Independent Claim
21 words
A method according to claims 3 or 4 wherein the salt is an alkali metal salt or a heavy metal salt.
Claim 7
Independent Claim
22 words
A method according to any one of claims 1 to 5 wherein a buffer is used in combination with the chelating agent.
Claim 8
Independent Claim
25 words
A method according to any one of claims 1 to 6 wherein the chelating agent is used in an amount of 1 mM or more.
Claim 9
Independent Claim
39 words
A method according to any one of claims 1 to 7 wherein the B-D-galactosidase and the chelating agent are brought into contact with each other at a pH of from 9 to 11.5, preferably of from 10 to 10.5.
Claim 10
Independent Claim
21 words
A method according to any one of claims 1 to 8 wherein the β-D-galactosidase is in free form or insolubilized form.
References Cited (Backward Citations)
No citation records indexed.
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