EP0073062A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Plasmid, process for its preparation and its use as vector
Assignee: KH Neochem Co Ltd
•
Filing: 1982-08-25
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Granted: —
•
Expires: 2002-08-25
Abstract
Abstract Disclosed is a novel vector, plasmid pCG2, and a process of producing plasmid pCG2 from Corynebacterium glutamicum.
Inventors
- 👤 Ryoichi Katsumata
- 👤 Tetsuo Oka
- 👤 Akira Furuya
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 19:19
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/77
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
19 words
Plasmid pCG2 characterized by a molecular weight of about 6.6 kilobases and the following cleavage sites for restriction endonucleases:
Claim 2
Dependent Claim
19 words
Plasmid pCG2 characterized by a molecular weight of about 6.6 kilobases and the following cleavage sites for restriction endonucleases:
Claim 3
Dependent Claim
28 words
A process for producing plasmid pCG2 which comprises culturing Corynebacterium glutamicum 225-218 in a nutrient medium, disrupting the cultured cells and recovering plasmid pCG2 from the disrupted cells.
Claim 4
Dependent Claim
28 words
A process for producing plasmid pCG2 which comprises culturing Corynebacterium glutamicum 225-218 in a nutrient medium, disrupting the cultured cells and recovering plasmid pCG2 from the disrupted cells.
Claim 5
Dependent Claim
12 words
A microorganism belonging to the species Corynebacterium glutamicum and carrying plasmid pCG2.
Claim 6
Dependent Claim
12 words
A microorganism belonging to the species Corynebacterium glutamicum and carrying plasmid pCG2.
Claim 7
Dependent Claim
13 words
A microorganism according to claim 3, which is Corynebacterium glutamicum 225-218, ATCC 31832.
Claim 8
Dependent Claim
20 words
A microorganism belonging to the genus Corynebacterium or a related microorganism which carries plasmid pCG2 isolated from Corynebacterium glutamicum 225-218.
Claim 9
Dependent Claim
20 words
A microorganism belonging to the genus Corynebacterium or a related microorganism which carries plasmid pCG2 isolated from Corynebacterium glutamicum 225-218.
Claim 10
Dependent Claim
14 words
The plasmid pCG2 according to claim 1 which is isolated from Corynebacterium glutamicum 225-218.
Claim 11
Dependent Claim
21 words
A vector plasmid pCG2 characterized by a molecular weight of about 6.6 kilobases and the following cleavage sites for restriction endonucleases:
Claim 12
Dependent Claim
21 words
A vector plasmid pCG2 characterized by a molecular weight of about 6.6 kilobases and the following cleavage sites for restriction endonucleases:
Claim 13
Dependent Claim
17 words
The vector plasmid pCG2 according to claim 7 which is plasmid pCG2 isolated from Corynebacterium glutamicum 225-218.
Claim 14
Dependent Claim
23 words
A derivative of the plasmid defined in claim 1 wherein a region of said plasmid is deleted and/or another DNA fragment is inserted.
References Cited (Backward Citations)
No citation records indexed.
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