EP0068763A2
Expired / Lapsed
EP
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Recombinant monoclonal antibodies
Assignee: The University of Texas System
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Filing: 1982-06-18
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Granted: —
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Expires: 2002-06-18
Abstract
Abstract Bifunctional antibodies are produced by trioma or quadroma cells formed by somatic cell fusion of a hybridoma with a lymphocyte or another hybridoma respectively.
Inventors
- 👤 Christopher L. Reading
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 18:53
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/46; C07K16/468; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S436/00; Y10S436/804; Y10S436/819; Y10S530/00; Y10S530/806; Y10S530/808; Y10S530/809; Y10S530/863; Y10S530/864
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
13 words
An antibody characterised in that it has binding affinity for two different antigens.
Claim 2
Dependent Claim
13 words
An antibody characterised in that it has binding affinity for two different antigens.
Claim 3
Dependent Claim
22 words
An antibody as claimed in Claim 1, wherein one of the two antigens is a tumor-specific antigen, cell-specific antigen, or tissue-specific antigen,
Claim 4
Dependent Claim
19 words
An antibody as claimed in Claim 1 or Claim 2 wherein one of the two antigens is an enzyme.
Claim 5
Dependent Claim
19 words
An antibody as claimed in Claim 1 or Claim 2 wherein one of the two antigens is a hapten.
Claim 6
Dependent Claim
28 words
An antibody as claimed in Claim 1 or Claim 2 wherein one of the two antigens is the toxic subunit of Ricinus communis toxin or is trinitrophenol- specific.
Claim 7
Dependent Claim
12 words
An antibody as claimed in Claim 4 wherein the hapten is radioactively-labelled.
Claim 8
Dependent Claim
32 words
An antibody as claimed in Claim 1 wherein one of the two antigens is the B subunit of human chorionic gonadotropin and the other antigen is a hapten or is horseradish peroxidase.
Claim 9
Dependent Claim
20 words
An antibody as claimed in Claim 1 or Claim 2 wherein one of the two antigens is fluorescein or rhodamine.
Claim 10
Dependent Claim
21 words
An antibody as claimed in Claim 8 wherein one of the two antigens is fluorescein and the other antigen is rhodamine.
Claim 11
Independent Claim
55 words
A method of producing a monoclonal antibody comprising incubating a hybrid cell in culture or in the peritoneal cavity of a mouse, and separating soluble protein from the culture supernatant or ascites fluid, respectively, characterised in that the hybrid cell is a trioma or quadroma cell and the antibody produced is a recombinant monoclonal antibody.
Claim 12
Independent Claim
55 words
A method of producing a monoclonal antibody comprising incubating a hybrid cell in culture or in the peritoneal cavity of a mouse, and separating soluble protein from the culture supernatant or ascites fluid, respectively, characterised in that the hybrid cell is a trioma or quadroma cell and the antibody produced is a recombinant monoclonal antibody.
Claim 13
Dependent Claim
54 words
A method as claimed in Claim 10, further comprising the step of passing the soluble protein sequentially through two affinity chromatographic columns each bearing one of two antigens for which the recombinant monoclonal antibody has binding affinity, immobilized on the column and eluting recombinant monoclonal antibody from each column with a chaotropic ion solution.
Claim 14
Dependent Claim
40 words
A method as claimed in Claim 10 or Claim 11 wherein the hybrid cell is a quadroma cell formed by fusing two hybridoma cell lines under conditions permitting selection of the fusion product in the presence of the parental hybridomas.
Claim 15
Dependent Claim
42 words
A method as claimed in Claim 10 or Claim 11 wherein the hybrid cell is a trioma cell formed by fusing hybridoma and immune spleen cell lines under conditions permitting selection of the fusion product in the presence of the parental cells.
Claim 16
Dependent Claim
4 words
A trioma or quadroma.
Claim 17
Dependent Claim
4 words
A trioma or quadroma.
Claim 18
Dependent Claim
42 words
A cell culture formed by somatic cell fusion of two different parental cells characterised in that it is a trioma formed by somatic cell fusion of a hybridoma and a lymphocyte or a quadroma formed by somatic cell fusion of two hybridomas.
Claim 19
Dependent Claim
42 words
A cell culture formed by somatic cell fusion of two different parental cells characterised in that it is a trioma formed by somatic cell fusion of a hybridoma and a lymphocyte or a quadroma formed by somatic cell fusion of two hybridomas.
References Cited (Backward Citations)
No citation records indexed.
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