EP0067029A2
Expired / Lapsed
EP
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Oxygen carrier
Assignee: Ajinomoto Co Inc
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Filing: 1982-06-02
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Granted: —
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Expires: 2002-06-02
Abstract
Abstract A modified haemoglobin for use as an oxygen carrier in a blood substitute is prepared by covalently bonding haemoglobin or a haemoglobin derivative to a polyalkylene glycol, via an amide bond. A carboxyl group is first introduced onto the polyalkylene glycol, and is then reacted with an amino group of the haemoglobin or haemoglobin derivative to form the amide bond.
Inventors
- 👤 Yuji Iwashita
- 👤 Keiji Iwasaki
- 👤 Katsumi Ajisaka
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 00:30
CPC Classifications
C; C08; C08G; C08G65/00; C08G65/02; C08G65/32; C08G65/329; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/56; A61K47/59; A61K47/60; A61P; A61P7/00; A61P7/08; C07; C07K; C07K14/00; C07K14/795; C07K14/805; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
39 words
An oxygen carrier comprising haemoglobin or a haemoglobin derivative covalently bonded to a polymer (such as a polyalkylene glycol or polyether), characterised in that the haemoglobin or haemoglobin derivative is covalently bonded to said polymer via an amide bond.
Claim 2
Dependent Claim
39 words
An oxygen carrier comprising haemoglobin or a haemoglobin derivative covalently bonded to a polymer (such as a polyalkylene glycol or polyether), characterised in that the haemoglobin or haemoglobin derivative is covalently bonded to said polymer via an amide bond.
Claim 3
Dependent Claim
25 words
An oxygen carrier as claimed in claim 1, wherein said polymer is polyethylene glycol, polypropylene glycol or a copolymer of ethylene oxide and propylene oxide.
Claim 4
Dependent Claim
73 words
An oxygen carrier as claimed in claim 2, wherein one or at least one hydroxyl group of said polymer is substituted by (a) a radical derived from an alcohol having from 1 to 16 carbon atoms, (b) a radical. derived from a carboxylic acid having from 2 to 18 carbon atoms, or (c) a radical derived from an amine having from 1 to 18 carbon atoms; or wherein said polymer is not substituted.
Claim 5
Dependent Claim
33 words
An oxygen carrier as claimed in any of claims 1 to 3, said amide bond having been formed by the use of a monohalogenated carboxylic acid, a dicarboxylic acid, or other carboxylic acid.
Claim 6
Dependent Claim
38 words
An oxygen carrier as claimed in any of claims 1 to 4, wherein the haemoglobin derivative is haemoglobin modified with pyridoxal or a derivative thereof, or with a sugar phosphate, or with 2,3-phosphoglyceric acid or a derivative thereof.
Claim 7
Dependent Claim
33 words
An oxygen carrier as claimed in claim 5, wherein the derivative is the pyridoxal-5'-phosphate derivative of haemoglobin, the pyridoxal-5'-sulphate derivative of haemoglobin, the 2-nor-2-formylpyridoxal-5'-phosphate derivative of haemoglobin, or the glucose-6-phosphate derivative of haemoglobin.
Claim 8
Dependent Claim
23 words
An oxygen carrier as claimed in any claims 1 to 6, wherein said polymer has a molecular weight of from 300 to 20000.
Claim 9
Dependent Claim
20 words
An oxygen carried as claimed in claim 7, wherein said polymer has a molecular weight of from 750 to 10000.
Claim 10
Independent Claim
53 words
A method of preparing an oxygen carrier, which comprises introducing at least one carboxyl group onto a polymer and covalently bonding the polymer to haemoglobin or a haemoglobin derivative by reaction of an amino group of the haemoglobin or haemoglobin derivative with the carboxyl group of the polymer to form an amide bond.
Claim 11
Independent Claim
53 words
A method of preparing an oxygen carrier, which comprises introducing at least one carboxyl group onto a polymer and covalently bonding the polymer to haemoglobin or a haemoglobin derivative by reaction of an amino group of the haemoglobin or haemoglobin derivative with the carboxyl group of the polymer to form an amide bond.
Claim 12
Dependent Claim
29 words
A method according to claim 9, wherein the carboxyl group is introduced onto the polymer by reaction of the polymer with a dicarboxylic acid or a monohalogenated carboxylic acid.
References Cited (Backward Citations)
No citation records indexed.
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