EP0060663A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
A process for transforming certain microorganisms, vectors and their production, and a process for producing certain substances using said vectors
Assignee: Shionogi and Co Ltd
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Filing: 1982-03-05
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Granted: —
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Expires: 2002-03-05
Abstract
Abstract The transformation of thermophilic gram-positive or gram-variable microorganisms using vector plasmids, vectors usable in such transformation and their preparation, and the use of such transformation followed by culturing of the transformed microorganisms to produce a desired substance derivable by phenotypical expression of a selected gene or genes incorporated in the vectors and hence in the genotype of the transformed microorganisms.
Inventors
- 👤 Aiba Shuichi
- 👤 Imanaka Tadayuki
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 19:51
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2408; C12N9/2411; C12N9/2414; C12N9/2417; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/75; C12N9/78; C12N9/86
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
24 words
A process for transformation of a microorganism by introduction of a plasmid thereinto characterized in that the microorganism is thermophilic and gram-positive or gram-variable.
Claim 2
Dependent Claim
24 words
A process for transformation of a microorganism by introduction of a plasmid thereinto characterized in that the microorganism is thermophilic and gram-positive or gram-variable.
Claim 3
Dependent Claim
18 words
A process as claimed in claim 1 further characterized in that the microorganism belongs to the genus Bacillus.
Claim 4
Dependent Claim
21 words
A process as claimed in claim 2 further characterized in that the microorganism is Bacillus stearothermophilus, Bacillus coagulans or Bacillus brevis.
Claim 5
Dependent Claim
27 words
A process as claimed in any one of claims 1 to 3 further characterized in that plasmid introduction is effected into the microorganism in a protoplast state.
Claim 6
Dependent Claim
28 words
A process as claimed in any one of claims 1 to 4 further characterized in that plasmid introduction is effected at a temperature of from 40° to 70°C.
Claim 7
Dependent Claim
53 words
A process as claimed in any one of claims 1 to 5 further characterized in that the plasmid comprises a vector which is introducible into a thermophilic gram-positive or gram-variable microorganism, is capable of replication in the body of the microorganism and its genotype is capable of successful phenotypical expression in the microorganism.
Claim 8
Dependent Claim
19 words
A process as claimed in claim 6 further characterized in that the vector is pTB 19 or pTB 53.
Claim 9
Dependent Claim
42 words
A vector characterized by being introducible into a gram-positive or gram-variable thermophilic microorganism, capable of replication in the body of the microorganism and its genotype being capble of successful phenotypical expression in the microorganism, the vector being derived from a thermophilic microorganism.
Claim 10
Dependent Claim
42 words
A vector characterized by being introducible into a gram-positive or gram-variable thermophilic microorganism, capable of replication in the body of the microorganism and its genotype being capble of successful phenotypical expression in the microorganism, the vector being derived from a thermophilic microorganism.
Claim 11
Dependent Claim
24 words
A vector as claimed in claim 8 further characterized in that the microorganism is as defined in any one of claims 2 to 4.
Claim 12
Dependent Claim
3 words
Vector pTB 19.
Claim 13
Dependent Claim
3 words
Vector pTB 19.
Claim 14
Dependent Claim
3 words
Vector pTB 53.
Claim 15
Dependent Claim
3 words
Vector pTB 53.
Claim 16
Dependent Claim
68 words
A process for producing vectors characterized by selecting microorganisms which are thermophilic and resistant to tetracycline or kanamycin and isolating therefrom plasmids of molecular weight 17.2 Mdal which carry genetic material encoding resistance both to tetracycline and to kanamycin and, if desired, cleaving said plasmids with a restricting endonuclease EcoRl and combining the resulting fragments using a DNA ligase to produce further plasmids of molecular weight 11.2 Mdal.
Claim 17
Dependent Claim
68 words
A process for producing vectors characterized by selecting microorganisms which are thermophilic and resistant to tetracycline or kanamycin and isolating therefrom plasmids of molecular weight 17.2 Mdal which carry genetic material encoding resistance both to tetracycline and to kanamycin and, if desired, cleaving said plasmids with a restricting endonuclease EcoRl and combining the resulting fragments using a DNA ligase to produce further plasmids of molecular weight 11.2 Mdal.
Claim 18
Dependent Claim
77 words
A process for producing a substance derivable by p henotypical expression of a selected gene or genes characterized by introducing a plasmid comprising said selected gene or genes and a vector as defined in claim 6 or claim 7 or a vector as claimed in any one of :laims 8 to 11 into a gram-positive or gram-variable thermophilic microorganism to transform the microorganism and culturing the resulting transformed microorganism to permits its genotype to be phenotypically expressed.
Claim 19
Dependent Claim
21 words
A process as claimed in claim 13 further characterised in that the substance is an enzyme, hormone, organic acid or antibiotic.
References Cited (Backward Citations)
No citation records indexed.
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