EP0060565A2
Expired / Lapsed
EP
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Fully synthetic cell culture medium
Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV
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Filing: 1982-03-17
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Granted: —
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Expires: 2002-03-17
Abstract
Abstract The invention relates to a fully synthetic, chemically defined cell-culture medium which in addition to the usual known components of culture media contains at least two unsaturated fatty acids and/or at least one flavanoid and/or at least one ubiquinone and/or vitamin U, and/or mevalolactone. The cell culture medium of the invention preferably contains no serum. However, it preferably contains at least one defined protein, especially highly purified, molecularly homogenous natural serum albumin. The cell culture medium is suitable especially for use in culturing eukaryontic cells, such as leukocytes, fibroblasts, endothelial and heart muscle cells.
Inventors
- 👤 Josef H. Dr. Wissler
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-03 19:30
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/0018; C12N5/0037; C12N5/0056; C12N2500/00; C12N2500/30; C12N2500/32; C12N2500/33; C12N2500/36; C12N2500/38; C12N2500/70; C12N2500/80; C12N2500/84
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
56 words
A fully synthetic cell culture medium containing salts, sugars, sugar derivatives, amino acids, amino acid derivatives, nucleosides and nucleoside bases, vitamins, vitaminoids, coenzymes, steroids and other usual additives in aqueous solution, characterized in that it additionally contains two unsaturated fatty acids and/or at least one flavanoid and/or at least one ubiquinone and/or vitamin U and/or mevalolactone.
Claim 2
Dependent Claim
56 words
A fully synthetic cell culture medium containing salts, sugars, sugar derivatives, amino acids, amino acid derivatives, nucleosides and nucleoside bases, vitamins, vitaminoids, coenzymes, steroids and other usual additives in aqueous solution, characterized in that it additionally contains two unsaturated fatty acids and/or at least one flavanoid and/or at least one ubiquinone and/or vitamin U and/or mevalolactone.
Claim 3
Dependent Claim
26 words
The medium according to claim 1, characterized in that it contains at least two unsaturated fatty acids and at least one of the other four components.
Claim 4
Dependent Claim
29 words
The medium according to claim 2, characterized in that it contains at least two unsaturated fatty acids, at least one flavanoid and at least one ubiquinone or vitamin U.
Claim 5
Independent Claim
26 words
The medium according to claims 1 and 2, characterized in that it contains at least one flavanoid, at least one ubiquinone and/or vitamin U and/or mevalolactone.
Claim 6
Independent Claim
18 words
The medium according to claims 1 to 4, characterized in that it contains at least one defined protein.
Claim 7
Dependent Claim
17 words
The medium according to claim 5, characterized in that it contains highly purified, molecularly homogenous serum albumin.
Claim 8
Independent Claim
78 words
The medium according to claims 1 and 6, characterized in that it additionally contains at least one of the substances: xylitol, D-xylose, D-galactose, glycerol and glucurono-γ-lactone and/or at least one of the substances: L-ornithine, sarcosine and taurine, and/or at least one of the substances: D,L-carnitine and L-carnosine, and/or at least one of the substances: cytidine, guanosine, 5-methylcytosine and uridine, and/or at least one of the substances: citric acid, succinic acid and pyruvic acid, and/or vitamin K 1 .
Claim 9
Independent Claim
27 words
The medium according to claims 1 to 7, characterized in that it contains the constituents in a quantitative ratio adjusted to the composition of natural blood plasma.
Claim 10
Independent Claim
21 words
The medium according to claims 1 to 8, characterized in that it is free of tensides, heavy metal salts and dyes.
Claim 11
Dependent Claim
23 words
The use of the cell culture medium according to claims 1 to 9 for the culture of eukaryontic cells, e.g. leukocytes from mammals.
References Cited (Backward Citations)
No citation records indexed.
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