EP0056249A2
Expired / Lapsed
EP
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Synthetic antigenic composition and process for making same
Assignee: New York Blood Center
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Filing: 1982-01-08
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Granted: —
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Expires: 2002-01-08
Abstract
Abstract A synthetic antigen composition consisting in a synthetic vaccine comprising a physiologically acceptable carrier on which is disposed a synthetic peptide residue containing a sequence of 6 amino acids corresponding to the sequence of such amino acids in a protein antigen or allergen where the greatest local average hydrophilicity of the antigen or allergen is found, said local hydrophilicity of said protein antigen or allergen determined by: A. assigning relative hydrophilicity values to the amino acids of the protein antigen or allergen in accordance with the relative relationship of such amino acids, B. determining the repetitive local average of hydrophilicity values at a plurality of points along the amino acid chain, C. determining from such points of repetitive averages the points of greatest local average hydrophilicity, said vaccine when free of an amino acid sequence corresponding to the entire protein antigen or allergen evoking a protective immunological response by stimulation of antibody formation against an antigen or allergen when introduced into a host animal.
Inventors
- 👤 Thomas Patrick Hopp
- 👤 Kenneth R. Woods
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 00:52
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; A; A61; A61K; A61K39/00; A61K39/385; A61K2039/60; A61K2039/6031; A61K2039/6093; C12; C12N; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
131 words
A synthetic vaccine comprising a physiologically acceptable carrier on which is disposed a synthetic peptide residue containing a sequence of 6 amino acids corresponding to the sequence of such amino acids in a protein antigen or allergen where the greatest local average hydrophilicity of the antigen or allergen is found, said local hydrophilicity of said protein antigen or allergen determined by: A. assigning relative hydrophilicity values to the amino acids of the protein antigen or allergen in accordance with the relative relationship of such amino acids as shown in the table below: said vaccine when free of an amino acid sequence corresponding to the entire protein antigen or allergen evoking a protective immunological response by stimmu- lation of antibody formation against an antigen or allergen when introduced into a host animal.
Claim 2
Dependent Claim
131 words
A synthetic vaccine comprising a physiologically acceptable carrier on which is disposed a synthetic peptide residue containing a sequence of 6 amino acids corresponding to the sequence of such amino acids in a protein antigen or allergen where the greatest local average hydrophilicity of the antigen or allergen is found, said local hydrophilicity of said protein antigen or allergen determined by: A. assigning relative hydrophilicity values to the amino acids of the protein antigen or allergen in accordance with the relative relationship of such amino acids as shown in the table below: said vaccine when free of an amino acid sequence corresponding to the entire protein antigen or allergen evoking a protective immunological response by stimmu- lation of antibody formation against an antigen or allergen when introduced into a host animal.
Claim 3
Dependent Claim
14 words
A vaccine according to claim 1, wherein said vaccine comprises a plurality of epitopes.
Claim 4
Dependent Claim
19 words
A vaccine according to claim 2, wherein said chain of amino acids comprises no more than 50 amino acids.
Claim 5
Dependent Claim
19 words
A vaccine according to claim 2, wherein said chain of amino acids comprises no more than 14 amino acids.
Claim 6
Dependent Claim
88 words
A vaccine according to claim 1, wherein said amino sequence comprises the amino acid sequence of an epitope which epitope corresponds to the amino acid sequence providing greatest local average hydrophilicity of at least one of the following protein antigens: hepatitis B antigen, human histocompatibility antigens, influenza hemagglutinin antigens, Fowl plague virus hemagglutinin, human chorionic gonadotropin B sub-unit, human B-2 microglobulin, human myelin basic protein, cholera toxin beta chain, E Coli toxins, streptococcal M protein, Trypanosoma brucci variant surface glycoprotein, herpes virus antigen, vaccina virus antigen, rabies antigen.
Claim 7
Dependent Claim
33 words
A synthetic vaccine according to claim 1, which is a vaccine for hepatitis B, said vaccine containing a peptide residue having the following sequence of amino acids: Lys Pro Thr Asp Gly Asn.
Claim 8
Dependent Claim
29 words
A synthetic vaccine according to claim 6, wherein said sequence of amino acids comprises the following sequence: Aba Aba Thr Lys Pro Thr Asp Gly Asn Aba Thr Aba.
Claim 9
Dependent Claim
30 words
A synthetic vaccine according to claim 6, wherein said sequence of amino acids comprises the following sequence : Cys Cys Thr Lys Pro Thr Asp Gly Asn Cys Thr Cys.
Claim 10
Dependent Claim
19 words
A vaccine according to claim 6, characterized by the absence of other peptide sequences of the hepatitis B virion.
Claim 11
Dependent Claim
23 words
A vaccine according to claim 9, wherein there are at least three amino acids on either side of said sequence of amino acids.
Claim 12
Dependent Claim
27 words
A vaccine according to claim 10, wherein the chain of the peptide which evokes a protective immunological response against hepatitis B does not exceed 16 amino acids.
Claim 13
Dependent Claim
27 words
A vaccine according to claim 9, wherein the chain of the peptide which evokes a protective immunological response against hepatitis B does not exceed 14 amino acids.
Claim 14
Dependent Claim
27 words
A vaccine according to claim 9, wherein each peptide chain having the said sequence of amino acids comprises between 6 and 12 amino acids in the chain.
Claim 15
Dependent Claim
25 words
A vaccine according to claim 1, wherein said carrier comprises a protein, a polysaccharide, another vaccine, an organic polymer, an organic polymer of a polypeptide.
Claim 16
Dependent Claim
155 words
A process for synthesizing a synthetic vaccine which comprises: A. assigning relative hydrophilicity values to amino acids of a protein antigen or allergen, the relative hydrophilicity values being assigned in accordance with a relative relationship as shown in the table 1 above; B. determining the repetitive local average of hydrophilicity values for sets of amino acids in said antigen or allergen at a plurality of points along the amino acid chain; C. determining the sequence of 6-amino acids corresponding to the points along said protein antigen or allergen of greatest local average hydrophilicity on the basis of such averages; D. forming a residue containing said sequence of 6 amino acids which residue is free of amino acid sequence corresponding to the entire protein antigen or allergen; E. disposing said residue containing said sequence of 6 amino acids on a physiologically acceptable carrier and F. diluting the carrier-residue of step E with a physiologically acceptable medium.
Claim 17
Dependent Claim
155 words
A process for synthesizing a synthetic vaccine which comprises: A. assigning relative hydrophilicity values to amino acids of a protein antigen or allergen, the relative hydrophilicity values being assigned in accordance with a relative relationship as shown in the table 1 above; B. determining the repetitive local average of hydrophilicity values for sets of amino acids in said antigen or allergen at a plurality of points along the amino acid chain; C. determining the sequence of 6-amino acids corresponding to the points along said protein antigen or allergen of greatest local average hydrophilicity on the basis of such averages; D. forming a residue containing said sequence of 6 amino acids which residue is free of amino acid sequence corresponding to the entire protein antigen or allergen; E. disposing said residue containing said sequence of 6 amino acids on a physiologically acceptable carrier and F. diluting the carrier-residue of step E with a physiologically acceptable medium.
Claim 18
Dependent Claim
15 words
An immune globulin comprising a mono-specific antibody which provides protective immunization or decreases allergen sensitivity.
Claim 19
Dependent Claim
15 words
An immune globulin comprising a mono-specific antibody which provides protective immunization or decreases allergen sensitivity.
Claim 20
Dependent Claim
27 words
An immune globulin according to claim 16, which is free of antibodies which do not either provide protective immunization or decrease allergen sensitivity on a host animal.
Claim 21
Dependent Claim
24 words
An immune globulin according to claim 16, wherein there are a plurality of mono-specific antibodies which mono-specific antibodies are specific to different antigenic determinants.
Claim 22
Dependent Claim
25 words
An immune globulin according to claim 16, wherein said immune globulin comprises a plurality of mono-specific antibodies which are specific to different antigens or allergens-
Claim 23
Dependent Claim
22 words
A vaccine according to claim 1, comprising a residue having the following sequence of amino acids: Lys Pro Ser Asp Gly Asn.
References Cited (Backward Citations)
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