EP0053046A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Stabilisation of factor VIII activity in blood plasma
Assignee: Individual
•
Filing: 1981-11-26
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Granted: —
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Expires: 2001-11-26
Abstract
Abstract Fresh blood plasma that has been collected in the presence of a calcium chelating anticoagulant from fresh whole blood or by plasmapheresis is treated to stabilise its Factor VIII activity. The method resides in adding dissolved calcium and dissolved heparin to the freshly collected plasma in an amount sufficient to stabilise significantly the Factor VIII activity, the amount of calcium generally being sufficient substantially to restore the calcium in the blood plasma to substantially normal physiological levels and the amount of heparin generally being from 4 to 8 units per ml. Novel compositions of calcium and heparin are described.
Inventors
- 👤 Gail Ann Rock
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 06:56
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/70; A61K31/715; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/122; A01N1/126; A61K31/13; A61K31/14; A61K35/00; A61K35/12; A61K35/14; A61K35/16; A61K38/00; A61K38/16; A61K38/17; A61K38/36; A61K38/37; A61L; A61L2/00; A61L2/02; A61L2/04; A61L2103/00; A61L2103/05; A61L2103/09; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
55 words
A method for stabilising Factor VIII activity in fresh blood plasma that has been collected in the presence of a calcium chelating anticoagulant from fresh whole blood or by plasmapheresis characterised in that dissolved calcium and dissolved heparin are added to the collected plasma in an amount sufficient to stabilise significantly the Factor VIII activity.
Claim 2
Independent Claim
55 words
A method for stabilising Factor VIII activity in fresh blood plasma that has been collected in the presence of a calcium chelating anticoagulant from fresh whole blood or by plasmapheresis characterised in that dissolved calcium and dissolved heparin are added to the collected plasma in an amount sufficient to stabilise significantly the Factor VIII activity.
Claim 3
Dependent Claim
25 words
A method according to claim 1 characterised in that the dissolved calcium and dissolved heparin are added as a combined solution containing calcium and heparin.
Claim 4
Dependent Claim
37 words
A method according°to claim 1 or claim 2 characterised in that the calcium and heparin are added at a time not more than 6 hours subsequent to collection of the whole blood or subsequent to the plasmapheresis.
Claim 5
Independent Claim
22 words
A method according to any preceding claim characterised in that the calcium chelating anticoagulant is citrate phosphate dextrose or acid citrate dextrose.
Claim 6
Independent Claim
24 words
A method according to any preceding claim characterised in that the amount of heparin is 2 to 20, preferably 4 to 8,units per ml.
Claim 7
Independent Claim
17 words
A method according to any preceding claim in which the heparin is provided as sodium heparin solution.
Claim 8
Independent Claim
31 words
A method according to any preceding claim in which the amount of calcium added is sufficient to restore the level of calcium in blood plasma to a substantially normal physiological level.
Claim 9
Dependent Claim
25 words
A method according to claim 7 characterised in that the amount of calcium added is sufficient to provide substantially 5 mg% free calcium ion concentration.
Claim 10
Dependent Claim
75 words
A method according to claim 4 characterised in that a solution of calcium and heparin is prepared by combining sodium heparin, a water soluble calcium salt and water and is then added to the fresh blood plasma, the amount of calcium and heparin being such that the resultant concentrations in the plasma are approximately 5 mg calcium ion per 100 ml plasma and 4 to 12, preferably 4 to 8, units heparin per ml plasma.
Claim 11
Independent Claim
22 words
A method according to any preceding claim characterised in that it includes the subsequent step of separating Factor VIII from the plasma.
Claim 12
Dependent Claim
32 words
A composition that can be added to blood plasma to stabilise the Factor VIII activity, characterised in that the composition comprises a solution of heparin and a soluble calcium compound in water.
Claim 13
Dependent Claim
32 words
A composition that can be added to blood plasma to stabilise the Factor VIII activity, characterised in that the composition comprises a solution of heparin and a soluble calcium compound in water.
Claim 14
Dependent Claim
25 words
A composition according to claim 11 characterised in that it contains calcium and heparin in the relative proportions 1 mg calcium:40-400, preferably 80-160, units heparin.
References Cited (Backward Citations)
US4203891A
Cited
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