EP0052829A2
Expired / Lapsed
EP
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Immobilization of viable microorganisms
Assignee: Bayer Corp
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Filing: 1981-11-11
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Granted: —
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Expires: 2001-11-11
Abstract
Abstract A method for immobilizing viable microorganisms in kappa-carrageenan is disclosed. The method involves mixing the microorganisms and kappa-carrageenan together and gelling the mixture with an epihalohydrin alkylene polyamine polymer or with polyethyleneimine. Also disclosed are methods of producing citric acid and ethanol from immobilized microorganisms.
Inventors
- 👤 Gerald Baltzer Borglum
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 08:30
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C12N11/04; C12P; C12P7/00; C12P7/02; C12P7/04; C12P7/06; C12P7/40; C12P7/44; C12P7/48; Y; Y02; Y02E; Y02E50/00; Y02E50/10
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
60 words
A method of preparing immobilized viable microorganisms which comprises the steps of mixing microorganisms with an aqueous solution of kappa-carrageenan at a temperature of about 25 to 50°C and gelling the mixture by the addition of a compound selected from the group consisting of an epihalohydrin-alkylene polyamine polymer and polyethyleneimine, to form a gel matrix containing said immobilized viable microorganisms.
Claim 2
Independent Claim
60 words
A method of preparing immobilized viable microorganisms which comprises the steps of mixing microorganisms with an aqueous solution of kappa-carrageenan at a temperature of about 25 to 50°C and gelling the mixture by the addition of a compound selected from the group consisting of an epihalohydrin-alkylene polyamine polymer and polyethyleneimine, to form a gel matrix containing said immobilized viable microorganisms.
Claim 3
Dependent Claim
21 words
A method as claimed in claim 1 wherein the microorganism is selected from the group consisting of yeasts, bacteria and fungi.
Claim 4
Dependent Claim
21 words
A method as claimed in claim 1 wherein the epihalohydrin-alkylene polyamine polymer has a molecular weight of from 4,000 to 50,000.
Claim 5
Dependent Claim
19 words
A method as claimed in claim 1 wherein the polyethyleneimine has a molecular weight of from 300 to 50,000.
Claim 6
Dependent Claim
13 words
A method as claimed in claim 2 wherein the yeast is Saccharomyces cerevisiae.
Claim 7
Dependent Claim
13 words
A method as claimed in claim 2 wherein the fungi is Aspergillus niger.
Claim 8
Independent Claim
47 words
A method of preparing immobilized catalytically active Aspergillus niger microorganisms which comprises the steps of mixing Aspergillus niger microorganisms with an aqueous solution of kappa-carrageenan at a temperature of about 40°C and gelling the mixture by the addition of polyethyleneimine having a molecular weight of about 1200.
Claim 9
Independent Claim
47 words
A method of preparing immobilized catalytically active Aspergillus niger microorganisms which comprises the steps of mixing Aspergillus niger microorganisms with an aqueous solution of kappa-carrageenan at a temperature of about 40°C and gelling the mixture by the addition of polyethyleneimine having a molecular weight of about 1200.
Claim 10
Independent Claim
73 words
A method of producing citric acid which comprises the steps of contacting a carbohydrate-containing substrate with immobilized Aspergillus niger microorganism cells and recovering citric acid therefrom, said immobilized cells having been produced by mixing Aspergillus niger microorganisms with an aqueous solution of-kappa-carrageenan at a temperature of about 25 to 50°C, and gelling the resulting mixture by the addition of a compound selected from the group consisting of an epihalohydrin-alkylene polyamine polymer and polyethyleneimine.
Claim 11
Independent Claim
73 words
A method of producing citric acid which comprises the steps of contacting a carbohydrate-containing substrate with immobilized Aspergillus niger microorganism cells and recovering citric acid therefrom, said immobilized cells having been produced by mixing Aspergillus niger microorganisms with an aqueous solution of-kappa-carrageenan at a temperature of about 25 to 50°C, and gelling the resulting mixture by the addition of a compound selected from the group consisting of an epihalohydrin-alkylene polyamine polymer and polyethyleneimine.
Claim 12
Independent Claim
68 words
A method of producing citric acid which comprises the steps of contacting a carbohydrate-containing substrate with immobilized Aspergillus niger microorganism cells and recovering citric acid therefrom, said immobilized cells having been produced by mixing Aspergillus niger microorganisms with an aqueous solution of kappa-carrageenan at a temperature of about 25 to 50°C, and gelling the mixture by the addition of a polyethyleneimine having a molecular weight of about 1200.
Claim 13
Independent Claim
68 words
A method of producing citric acid which comprises the steps of contacting a carbohydrate-containing substrate with immobilized Aspergillus niger microorganism cells and recovering citric acid therefrom, said immobilized cells having been produced by mixing Aspergillus niger microorganisms with an aqueous solution of kappa-carrageenan at a temperature of about 25 to 50°C, and gelling the mixture by the addition of a polyethyleneimine having a molecular weight of about 1200.
Claim 14
Independent Claim
60 words
A method of preparing immobilized catalytically active Saccharomyces cerevisiae microorganisms which comprises the steps of mixing Saccharomyces cerevisiae microorganisms with an aqueous solution of kappa-carrageenan at a temperature of about 40°C and gelling the mixture by the addition of a compound selected from the group consisting of an epihalohydrin-alkylene polyamine polymer and polyethyleneimine having a molecular weight of about 50,000.
Claim 15
Independent Claim
60 words
A method of preparing immobilized catalytically active Saccharomyces cerevisiae microorganisms which comprises the steps of mixing Saccharomyces cerevisiae microorganisms with an aqueous solution of kappa-carrageenan at a temperature of about 40°C and gelling the mixture by the addition of a compound selected from the group consisting of an epihalohydrin-alkylene polyamine polymer and polyethyleneimine having a molecular weight of about 50,000.
Claim 16
Independent Claim
71 words
A method of producing ethanol which comprises the steps of contacting a fermentable substrate with immobilized Saccharomyces cerevisiae microorganism cells and recovering ethanol therefrom, said immobilized cells having been produced by mixing Saccharomyces cerevisiae microorganisms with an aqueous solution of kappa-carrageenan at a temperature of about 25 to 50°C, and gelling the mixture by the addition of a compound selected from the group consisting of an epihalohydrin-alkylene polyamine polymer and polyethyleneimine.
Claim 17
Independent Claim
71 words
A method of producing ethanol which comprises the steps of contacting a fermentable substrate with immobilized Saccharomyces cerevisiae microorganism cells and recovering ethanol therefrom, said immobilized cells having been produced by mixing Saccharomyces cerevisiae microorganisms with an aqueous solution of kappa-carrageenan at a temperature of about 25 to 50°C, and gelling the mixture by the addition of a compound selected from the group consisting of an epihalohydrin-alkylene polyamine polymer and polyethyleneimine.
Claim 18
Independent Claim
66 words
A method of producing ethanol which comprises the steps of contacting a fermentable substrate with immobilized Saccharomyces cerevisiae microorganism cells and recovering ethanol therefrom, said immobilized cells having been produced by mixing Saccharomyces cerevisiae microorganisms with an aqueous solution of kappa-carrageenan at a temperature of about 25 to 50°C, and gelling the mixture by the addition of a polyethyleneimine having a molecular weight of about 1200.
Claim 19
Independent Claim
66 words
A method of producing ethanol which comprises the steps of contacting a fermentable substrate with immobilized Saccharomyces cerevisiae microorganism cells and recovering ethanol therefrom, said immobilized cells having been produced by mixing Saccharomyces cerevisiae microorganisms with an aqueous solution of kappa-carrageenan at a temperature of about 25 to 50°C, and gelling the mixture by the addition of a polyethyleneimine having a molecular weight of about 1200.
References Cited (Backward Citations)
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