EP0038529A1
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Composition, method, and element for the assay of neuraminic acids
Assignee: Eastman Kodak Co
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Filing: 1981-04-15
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Granted: —
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Expires: 2001-04-15
Abstract
Abstract A composition, method, and element for assaying a neuraminic acid employs a neuraminic acid aldolase, pyruvate oxidase and an electron acceptor. A sample containing a neuraminic acid can be contacted with the composition and the product detected spectrophotometrically.
Inventors
- 👤 Roy Eugene Snoke
- 👤 Theodore Walter Esders
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 04:23
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/34; C12Q1/527; G; G01; G01N; G01N2333/00; G01N2333/90; G01N2333/988; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
23 words
A composition for the assay of neuraminic acids in aqueous liquids characterized by a neuraminic acid aldolase, pyruvate oxidase, and an electron acceptor.
Claim 2
Dependent Claim
23 words
A composition for the assay of neuraminic acids in aqueous liquids characterized by a neuraminic acid aldolase, pyruvate oxidase, and an electron acceptor.
Claim 3
Dependent Claim
34 words
The composition of claim 1 wherein the neuraminic acid aldolase comprises at least 0.1 unit, the pyruvate oxidase comprises at least 0.005 unit, and the electron acceptor comprises from 0.02 millimolar to 10 millimolar.
Claim 4
Dependent Claim
19 words
The composition of either claim I or claim 2 wherein the neuraminic acid aldolase is N-acetyl neuraminic acid aldolase.
Claim 5
Dependent Claim
14 words
The composition of any of claims 1-3 wherein the electron acceptor is a dichloroindophenol.
Claim 6
Dependent Claim
11 words
The composition of claim 1 wherein the electron acceptor is oxygen.
Claim 7
Dependent Claim
14 words
The composition of any of claims 1-5 which is aqueous and includes a buffer.
Claim 8
Dependent Claim
14 words
The composition of claim 6 buffered to a pH of from 5 to 9.
Claim 9
Dependent Claim
10 words
The composition of any of claims 1-7 further containing neuraminidase.
Claim 10
Dependent Claim
12 words
The composition of claim 8 containing at least 0.01 unit of neuraminidase.
Claim 11
Dependent Claim
14 words
The composition of any of claims 1-9 wherein the neuraminic acid is sialic acid.
Claim 12
Independent Claim
45 words
A method for the detection of a neuraminic acid characterized by the steps of: (a) bringing a sample for analysis and a composition of any of claims 1-9 into contact in an aqueous medium to form a detectable product; and (b) determining the detectable product.
Claim 13
Dependent Claim
28 words
An element for the detection of a neuraminic acid characterized by a support having thereon at least one reagent layer containing a composition of any of claims 1-9.
Claim 14
Dependent Claim
30 words
The element of claim 12 including two reagent layers, the first reagent layer comprising pyruvate oxidase and an electron acceptor, and the second reagent layer comprising a neuraminic acid aldolase.
References Cited (Backward Citations)
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