Patent US5078745A successfully refreshed from Google Patents.
EP0036699B1
Expired / Lapsed
EP
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Extraction of poly-beta-hydroxybutyric acid
Assignee: Imperial Chemical Industries Ltd
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Filing: 1980-02-14
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Granted: 1983-02-02
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Expires: 2000-02-14
Abstract
No abstract record available.
Inventors
- 👤 Paul Arthur Holmes
- 👤 Leonard Feredrick Wright
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-24 09:19
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/62; C12P7/625; C08; C08G; C08G63/00; C08G63/88; C08G63/89
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
37 words
A process for the extraction of poly-β-hydroxybutyric acid (PHB) from bacterial cells containing PHB comprising contacting the bacterial cells with 1,2-dichloroethane at a temperature below 40°C and separating the PHB-containing solvent phase from the bacterial cell residue.
Claim 2
Dependent Claim
37 words
A process for the extraction of poly-β-hydroxybutyric acid (PHB) from bacterial cells containing PHB comprising contacting the bacterial cells with 1,2-dichloroethane at a temperature below 40°C and separating the PHB-containing solvent phase from the bacterial cell residue.
Claim 3
Dependent Claim
29 words
A process according to claim 1 in which an aqueous suspension of the cells is subjected to a cell disruption step prior to contact of the cells with 1,2-dichloroethane.
Claim 4
Dependent Claim
25 words
A process according to any one of claims 1 and 2 in which the 1,2-dichloroethane is contacted with an aqueous suspension of the bacterial cells.
Claim 5
Dependent Claim
49 words
A process according to claim 3 wherein the pH of the aqueous suspension is adjusted to within 0.5 units of pH of the isoelectric point of the cells, or of the disrupted cells if the cells are subjected to a cell disruption step, prior to contact with the 1,2-dichloroethane.
Claim 6
Dependent Claim
26 words
A process according to any one of claims 1 to 4 in which the weight of 1,2-dichioroethane is 10 to 100 times the cell dry weight.
Claim 7
Dependent Claim
36 words
A process according to any one of claims 1 to 5 in which the PHB is separated from the 1,2-dichloroethane by precipitation into a liquid in which PHB is insoluble and with which 1,2--dichloroethane is miscible.
References Cited (Backward Citations)
No citation records indexed.
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