EP0017565B1
Expired / Lapsed
EP
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Process for the inclusion of microorganisms in a polymer matrix and product so obtained
Assignee: Rhone Poulenc Recherches SA
•
Filing: 1980-03-27
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Granted: 1984-10-24
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Expires: 2000-03-27
Abstract
No abstract record available.
Inventors
- 👤 Gérard Jung
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-27 05:11
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/04; C05; C05F; C05F11/00; C05F11/08; C12N11/00; C12N11/02; C12N11/10; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/878
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
90 words
A process for producing micro-organisms which are included in a matrix formed by a gel of polymers based on at least one polymer of the polysaccharides group by bringing together the polymers in aqueous solution and the micro-organism(s) in the culture medium thereof; followed by at least partial cross-linking, characterised in that added to the gel produced is a substance having a high water absorption capacity, and that drying is effected so as to achieve a water content in the gel + absorbing agent mixture that is higher than 50%.
Claim 2
Dependent Claim
90 words
A process for producing micro-organisms which are included in a matrix formed by a gel of polymers based on at least one polymer of the polysaccharides group by bringing together the polymers in aqueous solution and the micro-organism(s) in the culture medium thereof; followed by at least partial cross-linking, characterised in that added to the gel produced is a substance having a high water absorption capacity, and that drying is effected so as to achieve a water content in the gel + absorbing agent mixture that is higher than 50%.
Claim 3
Dependent Claim
32 words
A process according to claim 1 characterised by forming a solution of polysaccharides in the hot condition before bringing together the polysaccharide in aqueous solution and the micro-organisms in their culture medium.
Claim 4
Dependent Claim
20 words
A process according to claim 2 characterised in that the heating operation is carried out at between 70 and 80°C.
Claim 5
Dependent Claim
31 words
A process according to claim 2 or claim 3 characterised in that the polymers and the micro-organism(s) are brought together at a temperature of the order of from 40 to 45°C.
Claim 6
Dependent Claim
19 words
A process according to claim 2 and claim 3 characterised in that the cross-linking step is effected by cooling.
Claim 7
Dependent Claim
36 words
A process according to claim 1 characterised in that the cross-linking step is effected by adding a metal salt such as of iron or aluminium, which may or may not be chelated by a polyhydric alcohol.
Claim 8
Dependent Claim
24 words
A process according to claim 1 or claim 6 characterised in that the operation of bringing the substances together is effected at ambient temperature.
Claim 9
Dependent Claim
55 words
A process according to any one of the preceding claims characterised in that the polymer is based on a heteropolysaccharide having a high molecular weight, wherein one of the polysaccharides is produced by fermentation of a carbohydrate by a bacterium of the genus Xanthomonas or Arthrobacter or by a fungus belonging to the genus Sclerotium.
Claim 10
Dependent Claim
27 words
A process according to claim 7 characterised in that the polysaccharide gel also comprises at least one other polysaccharide from the group of gums of natural origin.
Claim 11
Dependent Claim
20 words
A process according to claim 9 characterised in that the polysaccharides are selected from: alginates, kappa carragheenanes and carob flour.
Claim 12
Dependent Claim
26 words
A process according to claim 1 or claim 4 characterised in that the culture medium or the microbial suspension is added separately to each polysaccharide solution.
Claim 13
Dependent Claim
20 words
A process according to any one of the preceding claims characterised in that the micro-organism is of the genus Rhizobium.
Claim 14
Dependent Claim
17 words
A process according to claim 1 characterised in that the drying operation is carried out in air.
Claim 15
Dependent Claim
38 words
A process according to claim 1 characterised in that the added substance comprises silica so that the proportion of residual water in the gel + silica mixture is from 50 to 70 g per 100 g of silica.
Claim 16
Dependent Claim
29 words
A process according to one of claims 1 and -14 characterised in that the percentage of additive is from 10 to 120% by weight with respect to the gel.
Claim 17
Dependent Claim
49 words
A process according to one of claims 1 and 14 characterised in that the additive comprises a precipitation silica with a BET surface area of 200 m 2 /g, a CTAB surface area of 90 m 2 /g, and dioctylphthalate oil absorption of 340-346 cm 3 /1 00 g.
Claim 18
Dependent Claim
27 words
A micro-organism included in a polymer matrix characterised in that it is produced by carrying out the process according to one of claims 1 to 1 6.
References Cited (Backward Citations)
EP0028900A1
Cited
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