EP0008969A2
Expired / Lapsed
EP
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Vaccine and method of making
Assignee: Harvard University
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Filing: 1979-08-13
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Granted: —
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Expires: 1999-08-13
Abstract
Abstract Culturing Streptococcus agalactiae in a nutrient broth containing at least 10 g l of glucose while maintaining the pH at 6-8 by the addition of alkaline material produces polysaccharide antigen including a fraction having a molecular size from 8-11 x 10<sup>5</sup>d. By employing a broth free from constituents having a molecular size above 10<sup>5</sup>, the antigens can be separated from the broth in high yield after harvesting the organisms. Vaccination of humans with Streptococcus agalactiae polysaccharide antigens having molecular sizes above 5 x 10' produces in selected individuals specific antibody levels above 200 µg ml of antisera; globulins fractionated from such antisera contain most of the antibody and can be used for passive immunization.
Inventors
- 👤 Dennis L. Kasper
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-25 09:16
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/02; A61K39/09; A61K39/092; C; C07; C07K; C07K16/00; C07K16/12; C07K16/1267; C07K16/1275; C12; C12P; C12P19/00; C12P19/26; A61K38/00; Y; Y10; Y10S; Y10S435/00; Y10S435/961
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
68 words
In a method of making a polysaccharide vaccine against Group B streptococci Type Ia, Ic, II or III which comprises culturing the streptococci in a nutrient broth the improvement which comprises introducing into the broth at least 10 g/1 of glucose and maintaining the pH of the broth at pH 6-8 to provide polysaccharide antigen including a fraction having a molecular size above 0.8 X 10 6 .
Claim 2
Dependent Claim
68 words
In a method of making a polysaccharide vaccine against Group B streptococci Type Ia, Ic, II or III which comprises culturing the streptococci in a nutrient broth the improvement which comprises introducing into the broth at least 10 g/1 of glucose and maintaining the pH of the broth at pH 6-8 to provide polysaccharide antigen including a fraction having a molecular size above 0.8 X 10 6 .
Claim 3
Dependent Claim
26 words
A method as claimed in claim 1 in which the pH is maintained by introducing alkaline material into the broth during the growth of said streptococci.
Claim 4
Dependent Claim
18 words
A method as claimed in claim 1 in which the pH is maintained at pH 6.8 to 7.6.
Claim 5
Dependent Claim
21 words
A method as claimed in claim 1 in which the amount of glucose is from about 14 to about 16 g/l.
Claim 6
Dependent Claim
54 words
A method as claimed in claim 1 in which the nutrient broth is free from constituents having a molecular size above 10 at the beginning of culture, and polysaccharide antigen including a fraction having a molecular size from 8 to 11 X 10 5 is recovered from the broth after harvesting of the streptococci.
Claim 7
Dependent Claim
41 words
A method as claimed in claim 1 in which there is removed from the broth constituents having molecular sizes above 3 X 10 4 , and an amount of glucose is added to provide a concentration of 14 to 16 g/l.
Claim 8
Independent Claim
63 words
A method of making gamma globulin useful for immunization against Group B streptococci which comprises isolating from said streptococci a Group B-specific polysaccharide antigen having a molecular size of at least 5 X 10 5 , injecting said antigen into humans to produce antisera having specific antibody concentrations of at least 200 ug/ml, and fractionating said antisera to provide a hyperimmune globulin fraction.
Claim 9
Independent Claim
63 words
A method of making gamma globulin useful for immunization against Group B streptococci which comprises isolating from said streptococci a Group B-specific polysaccharide antigen having a molecular size of at least 5 X 10 5 , injecting said antigen into humans to produce antisera having specific antibody concentrations of at least 200 ug/ml, and fractionating said antisera to provide a hyperimmune globulin fraction.
Claim 10
Dependent Claim
21 words
A method as claimed in claim 7 in which said antigen has a molecular size above 0.8 X 10 6 .
Claim 11
Dependent Claim
68 words
A polysaccharide antigen in purified form specific to Type III Group B streptococci consisting essentially of repeating units having the following composition: in which GlcNAcp stands for N-acetyl glucosamine (in the pyranose form), Galp stands for galactose (in the pyranose form), Glcp stands for glucose (in the pyranose form) and a-D-NANA stands for sialic acid, said polysaccharide having a molecular size larger than 0.8 X 10 6 .
Claim 12
Dependent Claim
68 words
A polysaccharide antigen in purified form specific to Type III Group B streptococci consisting essentially of repeating units having the following composition: in which GlcNAcp stands for N-acetyl glucosamine (in the pyranose form), Galp stands for galactose (in the pyranose form), Glcp stands for glucose (in the pyranose form) and a-D-NANA stands for sialic acid, said polysaccharide having a molecular size larger than 0.8 X 10 6 .
Claim 13
Dependent Claim
71 words
A polysaccharide antigen in purified form specific to Type Ia and Type Ic Group B streptococci consisting essentially of repeating units having the following composition: in which GlcNAcp stands for N-acetyl glucosamine (in the pyranose form), Galp stands for galactose (in the pyranose form), Glcp stands for glucose (in the pyranose form) and a-D-NANA stands for sialic acid, said polysaccharide having a molecular size larger than 0.8 X 10 6 .
Claim 14
Dependent Claim
71 words
A polysaccharide antigen in purified form specific to Type Ia and Type Ic Group B streptococci consisting essentially of repeating units having the following composition: in which GlcNAcp stands for N-acetyl glucosamine (in the pyranose form), Galp stands for galactose (in the pyranose form), Glcp stands for glucose (in the pyranose form) and a-D-NANA stands for sialic acid, said polysaccharide having a molecular size larger than 0.8 X 10 6 .
Claim 15
Dependent Claim
24 words
Hyperimmune globulin fraction of human antisera having activity specific to Group B streptococci at a level of at least 2000 µg/ml of specific activity.
Claim 16
Dependent Claim
24 words
Hyperimmune globulin fraction of human antisera having activity specific to Group B streptococci at a level of at least 2000 µg/ml of specific activity.
Claim 17
Dependent Claim
32 words
A hyperimmune globulin fraction as claimed in claim 11 in a physiologically acceptable carrier at a dosage level from 0.3 to 1 ml at a concentration from 5 to 20% by weight.
Claim 18
Dependent Claim
42 words
A vaccine against Group B streptococcus comprising a physiologically acceptable non-toxic vehicle containing 10 to 1000 µg/ml of a polysaccharide antigen consisting essentially of sialic acid, galactose, glucose, and glucosamine and having a molecular size larger than 0.8 X 10 6 .
Claim 19
Dependent Claim
42 words
A vaccine against Group B streptococcus comprising a physiologically acceptable non-toxic vehicle containing 10 to 1000 µg/ml of a polysaccharide antigen consisting essentially of sialic acid, galactose, glucose, and glucosamine and having a molecular size larger than 0.8 X 10 6 .
Claim 20
Dependent Claim
43 words
A vaccine against Type Ia, Ic or III Group B streptococcus comprising a physiologically acceptable non-toxic vehicle containing 10 to 1000 µg/ml of the polysaccharide antigen as claimed in claim 9 for Type III or in claim 10 for Type Ia and Ic.
References Cited (Backward Citations)
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