EP0006694A2
Expired / Lapsed
EP
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Method of making a selected protein
Assignee: Nazionalizzazione biogen Inc
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Filing: 1979-06-04
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Granted: —
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Expires: 1999-06-04
Abstract
Abstract A plasmid or phage gene for a periplasmic or extracellular bacterial protein is cleaved, a doublestranded DNA sequence coding for a selected protein or portion thereof from a eukaryotic cell such as insulin is inserted in that cleaved gene by recombinant DNA techniques and used to transform a bacterium, and the excreted selected protein is collected.
Inventors
- 👤 Walter Gilbert
- 👤 Stephanie A. Broome
- 👤 Lydia J. Villa-Komaroff
- 👤 Argiris A. Efstratiadis
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-01 12:36
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/62; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/66; C12N9/00; C12N9/14; C12N9/78; C12N9/86; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/034; C07K2319/70; C07K2319/74; C07K2319/75; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/30
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
78 words
A method of making a selected protein or portion thereof by inserting DNA representing the selected protein or portion thereof into a bacterial gene,characterized by cleaving the bacterial gene for an extracellular or periplasmic carrier protein, inserting into the cleavage site by a recombinant step a non-bacterial DNA fragment which codes for the selected protein or portion thereof, transforming a bacterial host with the recombined gene,.and culturing the transformed bacteria to excrete the selected protein or portion thereof.
Claim 2
Independent Claim
78 words
A method of making a selected protein or portion thereof by inserting DNA representing the selected protein or portion thereof into a bacterial gene,characterized by cleaving the bacterial gene for an extracellular or periplasmic carrier protein, inserting into the cleavage site by a recombinant step a non-bacterial DNA fragment which codes for the selected protein or portion thereof, transforming a bacterial host with the recombined gene,.and culturing the transformed bacteria to excrete the selected protein or portion thereof.
Claim 3
Dependent Claim
32 words
The method according to claim 1, characterized in that the selected protein is a eukaryotic cell protein which contains a hydrophobic leader which is normally cleaved during excretion from the eukaryotic cell.
Claim 4
Dependent Claim
18 words
The method according to claim 1 or 2, characterized in that the carrier protein is E. coli penicillinase.
Claim 5
Independent Claim
18 words
The method according to any of claims 1 to 3, characterized in that the selected protein is insulin.
Claim 6
Dependent Claim
27 words
The method according to claim 1, characterized in that the carrier protein is E. coli penicillinase and the bacterial gene is cleaved at the Pst restriction site.
References Cited (Backward Citations)
No citation records indexed.
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