EP0005644A2
Expired / Lapsed
EP
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Production of urokinase
Assignee: Monsanto Co
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Filing: 1979-05-22
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Granted: —
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Expires: 1999-05-22
Abstract
Abstract The yield of urokinase produced by cell culture of kidney cells is improved by incorporating in the cell culture medium an elevated level of phenylalanine.
Inventors
- 👤 Mau-Jung Kuo
- 👤 Margaret Jean Reents
- 👤 Joseph Feder
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-26 16:12
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6462; C12Y; C12Y304/00; C12Y304/21; C12Y304/21073
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
45 words
A process for the production of urokinase by the culture of live kidney cells in an aqueous nutrient medium to which has been added a sufficient quantity of an amino acid to increase the yield of urokinase, characterised in that the amino acid is phenylalanine.
Claim 2
Dependent Claim
45 words
A process for the production of urokinase by the culture of live kidney cells in an aqueous nutrient medium to which has been added a sufficient quantity of an amino acid to increase the yield of urokinase, characterised in that the amino acid is phenylalanine.
Claim 3
Dependent Claim
29 words
A process according to Claim 1, in which the phenylalanine is used in an amount offrom about 0.3 to about 0.7 percent based on the weight of the medium.
Claim 4
Dependent Claim
26 words
A process according to Claim 2 in which the phenylalanine is used in an amount of about 0.5 percent based on the weight of the medium.
Claim 5
Dependent Claim
21 words
A process according to any of Claims 1 to 3 in which the cells are normal primary human embryonic kidney cells.
Claim 6
Dependent Claim
24 words
A process according to any of Claims 1 to 4 in which the medium comprises lactalbumin hydrolysate, human serum albumin, glucose and sodium bicarbonate.
References Cited (Backward Citations)
No citation records indexed.
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