Process for crystallization of glucose isomerase

Abstract

Abstract The invention concerns a stable glucose isomerase concentrate, in which the glucose isomerase is present as dissolved in a concentrated polyhydroxy compound containing water solution. The invention also concerns a process for the preparation of such a glucose isomerase concentrate, wherebya) a suitable salt is added to a partially purified glucose isomerase solution obtained from fermentation, so as to crystallize the glucose isomerase,b) the solution is cooled so as to promote crystallization of the glucose isomerase, and the crystal mass formed is separated, whereupon, if desired, one or several recrystallizations are performed, andc) a carbohydrate or a concentrated water solution of same is added to the crystal mass obtained, which said mass is dissolved, whereby a stable glucose isomerase concentrate is obtained. The glucose isomerase concentrate in accordance with the invention is used for immobilizing the glucose isomerase on a carrier material.

Inventors

• 👤 Kalevi J Visuri

Bibliographic Details