CA2011784A1
Expired / Lapsed
CA
⏱️ 0.0 years remaining
Method for the targetted change of a gene
Assignee: Max-Planck-Gesellschaft zur Förderung der Wissenschaften eV
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Filing: 1990-03-08
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Granted: —
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Expires: 2010-03-08
Abstract
Abstract A b s t r a c t For the targetted change of a gene within the genome of intact mammalian cells by homologous recombination a DNA sequence, which is homologous to the gene to be changed but, however, differs by at least one nucleotide from its sequence as a result of mutation, deletion, or insertion, is introduced into appropriate cells by microinjection and cells are isolated in which the resultant change can be detected.
Inventors
- 👤 Peter Gruss
- 👤 Andreas Zimmer
Bibliographic Details
Application Number
—
Jurisdiction
CA
Data Source
google_patents
Last Synchronized
2026-11-08 03:23
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/10; C12N15/102; C12N15/87; C12N15/90; C12N15/902; C12N15/907
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
75 words
Method for the targetted change of a gene within the genome of intact mammalian cells by homologous recombination, w h e r e i n a DNA sequence, which is homologous to the gene to be changed but, however, differs by at least one nucleotide from its sequence as a result of mutation, deletion, or insertion, is introduced into appropriate cells by microinjection and cells are isolated in which the resultant change can be detected.
Claim 2
Dependent Claim
75 words
Method for the targetted change of a gene within the genome of intact mammalian cells by homologous recombination, w h e r e i n a DNA sequence, which is homologous to the gene to be changed but, however, differs by at least one nucleotide from its sequence as a result of mutation, deletion, or insertion, is introduced into appropriate cells by microinjection and cells are isolated in which the resultant change can be detected.
Claim 3
Dependent Claim
22 words
Method as claimed in claim 1, w h e r e i n somatic stem cells are used as the mammalian cells.
Claim 4
Dependent Claim
26 words
Method as claimed in claim 1 or 2, w h e r e i n a PCR reaction is carried out to detect the resultant change.
Claim 5
Dependent Claim
43 words
Method as claimed in one of the claims 1 to 3, w h e r e i n the gene to be changed is a gene which causes symptoms of disease and a DNA sequence is used which corresponds to the healthy gene.
References Cited (Backward Citations)
No citation records indexed.
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