CA1189807A
Expired / Lapsed
CA
⏱️ 0.0 years remaining
Microbiological process for oxidation of alkanes, vinyl compounds and secondary alcohols
Assignee: ExxonMobil Technology and Engineering Co
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Filing: 1983-03-18
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Granted: 1985-07-02
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Expires: 2003-03-18
Abstract
Abstract ABSTRACT OF THE DISCLOSURE A substrate selected from alkanes, saturated alicyclic or aromatic hydrocarbons, alkenes, dienes, vinyl aromatic compounds or secondary alcohols is oxidized to the corresponding oxidation product(s) by contacting it in a reaction medium under aerobic condi-tions with microbial cells derived from a selected microorganism or a genetically engineered derivative or natural mutant thereof or an enzyme preparation prepared from the cells, wherein the microorganism has been previously grown under aerobic conditions in a nutrient medium containing a C2-C6 alkane or, if the substrate is a secondary alcohol, also a C2-C6 alkyl radical donating compound such as a linear alcohol or alkylamine.
Inventors
- 👤 Ching-Tsang Hou
- 👤 Allen I. Laskin
- 👤 Ramesh N. Patel
Bibliographic Details
Application Number
—
Jurisdiction
CA
Data Source
google_patents
Last Synchronized
2026-11-02 09:00
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/0004; C12N9/0006; C12P; C12P17/00; C12P17/02; C12P7/00; C12P7/02; C12P7/04; C12P7/06; C12P7/22; C12P7/24; C12P7/26; C12P7/28; Y; Y02; Y02E; Y02E50/00; Y02E50/10
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
322 words
A process for oxidizing a substrate selected from alkanes, saturated alicyclic or aromatic hydrocarbons, alkenes, dienes, vinyl aromatic compounds, or secondary alcohols to the corresponding oxidation product or products comprising contacting said substrate in a reaction medium under aerobic conditions with microbial cells derived from a microorganism, a gen-etically engineered derivative or natural mutant of the microorganism, or an enzyme preparation prepared from said cells, which microorganism, derivative, mutant or preparation exhibits oxygenase or dehydrogenase enzyme activity, until the corresponding product is produced in at least isolatable amounts, wherein the microorganism has been previously grown under aerobic conditions in a nutrient medium containing a compound which is a C2-C6 alkane or, if the substrate is an alcohol, the compound may additionally be a C2-C6 alkyl radical donating compound, and wherein said microorganism strains are selected from the group consisting of: Rhodococcus rhodochrous (Arthrobacter sp.) ATCC 19140 Pseudomonas aeruginosa (Alcaligenes sp.) ATCC 15525 Arthrobacter petroleophagus ATCC 21494 Arthrobacter simplex ATCC 21032 Pseudomonas aeruginosa (Brevibacterium insectiphilum) ATCC 1552 Brevibacterium sp. ATCC 14649 Brevibacterium fuscum ATCC 15993 Alcaligenes eutrophus (Hydrogenomonas eutropha) ATCC 17697 Mycobacterium album ATCC 29676 Rhodococcus rhodochrous (Mycobacterium rhodochrous) ATCC 29670 Rhodococcus rhodochrous (Mycobacterium rhodochrous) ATCC 29672 Rhodococcus sp. (Nocardia neoopaca) ATCC 21499 Pseudonomas crucurae NRRL B-1021 Pseudomonas fluorescens NRRL B-1244 Pseudomonas cepacia (Pseudomonas multivorans) ATCC 17616 Pseudomonas putida Type A ATCC 17453 Pseudomonas aeruginosa (Pseudomonas ligustri) ATCC 15522, wherein further if the substrate is an alkene, diene or vinyl aromatic compound, the microorganism strains may additionally be selected from the group consisting of: Mycobacterium parafrinicum ATCC 12670 Rhodococcus rhodochrous (Mycobacterium rhodochrous) ATCC 184, and wherein further if the substrate is a secondary alcohol, the microorganism strains may additionally be selected from the group consisting of: Mycobacterium paraffinicum ATCC 12670 Rhodococcus rhodochrous (Nocardia paraffinica) ATCC 21198, and wherein further if the substrate is an alkane or saturated alicyclic or aromatic hydrocarbon, the micro-organism strain may additionally be: Rhodococcus rhodochrous (Nocardia paraffinica) ATCC 21198.
Claim 2
Dependent Claim
322 words
A process for oxidizing a substrate selected from alkanes, saturated alicyclic or aromatic hydrocarbons, alkenes, dienes, vinyl aromatic compounds, or secondary alcohols to the corresponding oxidation product or products comprising contacting said substrate in a reaction medium under aerobic conditions with microbial cells derived from a microorganism, a gen-etically engineered derivative or natural mutant of the microorganism, or an enzyme preparation prepared from said cells, which microorganism, derivative, mutant or preparation exhibits oxygenase or dehydrogenase enzyme activity, until the corresponding product is produced in at least isolatable amounts, wherein the microorganism has been previously grown under aerobic conditions in a nutrient medium containing a compound which is a C2-C6 alkane or, if the substrate is an alcohol, the compound may additionally be a C2-C6 alkyl radical donating compound, and wherein said microorganism strains are selected from the group consisting of: Rhodococcus rhodochrous (Arthrobacter sp.) ATCC 19140 Pseudomonas aeruginosa (Alcaligenes sp.) ATCC 15525 Arthrobacter petroleophagus ATCC 21494 Arthrobacter simplex ATCC 21032 Pseudomonas aeruginosa (Brevibacterium insectiphilum) ATCC 1552 Brevibacterium sp. ATCC 14649 Brevibacterium fuscum ATCC 15993 Alcaligenes eutrophus (Hydrogenomonas eutropha) ATCC 17697 Mycobacterium album ATCC 29676 Rhodococcus rhodochrous (Mycobacterium rhodochrous) ATCC 29670 Rhodococcus rhodochrous (Mycobacterium rhodochrous) ATCC 29672 Rhodococcus sp. (Nocardia neoopaca) ATCC 21499 Pseudonomas crucurae NRRL B-1021 Pseudomonas fluorescens NRRL B-1244 Pseudomonas cepacia (Pseudomonas multivorans) ATCC 17616 Pseudomonas putida Type A ATCC 17453 Pseudomonas aeruginosa (Pseudomonas ligustri) ATCC 15522, wherein further if the substrate is an alkene, diene or vinyl aromatic compound, the microorganism strains may additionally be selected from the group consisting of: Mycobacterium parafrinicum ATCC 12670 Rhodococcus rhodochrous (Mycobacterium rhodochrous) ATCC 184, and wherein further if the substrate is a secondary alcohol, the microorganism strains may additionally be selected from the group consisting of: Mycobacterium paraffinicum ATCC 12670 Rhodococcus rhodochrous (Nocardia paraffinica) ATCC 21198, and wherein further if the substrate is an alkane or saturated alicyclic or aromatic hydrocarbon, the micro-organism strain may additionally be: Rhodococcus rhodochrous (Nocardia paraffinica) ATCC 21198.
Claim 3
Dependent Claim
12 words
Process of claim 1 wherein said C2-C6 alkane is a C2-C4 alkane.
Claim 4
Dependent Claim
10 words
Process of claim 1 wherein said C2-C6 alkane is propane.
Claim 5
Dependent Claim
14 words
Process of claims 1 or 2 wherein said strain is a Pseudomonas aeruginosa strain.
Claim 6
Dependent Claim
28 words
Process of any one of claims 1 to 3 wherein said substrate is a C2-C6 alkane, cyclohexane, benzene, a C2-C7 alkene, butadiene, styrene or a C3-C6 secondary alcohol.
Claim 7
Dependent Claim
22 words
Process of any one of claims 1 to 3 wherein said substrate is propane, n-butane, cyclohexane, benzene, ethylene, propylene, 2-propanol or 2-butanol.
Claim 8
Dependent Claim
28 words
Process of claim 1 wherein said C2-C6 alkyl radical donating compound is a C2-C4 compound selected from the group consisting of ethanol, propanol, butanol, ethylamine, propylamine and butylamine.
Claim 9
Dependent Claim
14 words
Process of claims 1 or 7 wherein said secondary alcohol is a C3-C5 alcohol.
Claim 10
Dependent Claim
11 words
Process of claim 1 wherein the oxidation is carried out batchwise.
Claim 11
Dependent Claim
14 words
Process of claim 1 wherein the oxidation is carried out in a continuous manner.
Claim 12
Dependent Claim
12 words
11 Process of claim 1 wherein said micro-bial cells are resting cells.
Claim 13
Dependent Claim
17 words
Process of claim 1 wherein said micro-bial cells are in the form of a resting cell suspension.
Claim 14
Dependent Claim
16 words
Process of claim 12 wherein said substrate is a C3-C6 alkane, a C2-C6 alkene or butadiene.
Claim 15
Dependent Claim
13 words
Process of claim 1 wherein said enzyme preparation is a cell-free soluble fraction.
Claim 16
Dependent Claim
33 words
Process of claim 1 wherein the substrate is an alkene, diene or vinyl aromatic compound and an electron carrier or other biochemical species which drives the oxidation is added to the reaction medium.
Claim 17
Dependent Claim
10 words
Process of claim 15 wherein the electron carrier is NADH.
Claim 18
Dependent Claim
16 words
Process of claims 15 or 16 wherein the electron carrier or other biochemical species is regen-erated.
References Cited (Backward Citations)
No citation records indexed.
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