CA1179927A
Expired / Lapsed
CA
⏱️ 0.0 years remaining
Test for esterase activity in a liquid sample
Assignee: Bayer Corp
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Filing: 1982-12-23
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Granted: 1984-12-27
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Expires: 2002-12-23
Abstract
Abstract ABSTRACT OF THE DISCLOSURE A composition, test device and method for determining the presence of leukocytes, esterase and/or esterase activity in a test sample is dis-closed. The composition comprises an ester which, when cleaved by esterolytic activity, produces a detectable response such as color formation. In addition the composition comprises the compound 3-quinuclidinol. The device is prepared by incor-porating a carrier matrix, such as paper, with the composition. The method comprises contacting a test sample with the device and observing a detect able response.
Inventors
- 👤 A. Christopher Skjold
- 👤 Lonnie R. Stover
- 👤 Robert W. Trimmer
Bibliographic Details
Application Number
—
Jurisdiction
CA
Data Source
google_patents
Last Synchronized
2026-11-01 13:28
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/44; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
69 words
A composition for determining the presence of esterase activity in a liquid test sample said composition comprising a chromogenic ester having the formula R - O - R' in which R is a moiety which produces a detectable response when the ester is cleaved through an esterase or esterase-like esterolytic reaction, and in which R' is an amino acid or peptide moiety having a nitro-gen protective group, and 3-quinuclidinol.
Claim 2
Dependent Claim
69 words
A composition for determining the presence of esterase activity in a liquid test sample said composition comprising a chromogenic ester having the formula R - O - R' in which R is a moiety which produces a detectable response when the ester is cleaved through an esterase or esterase-like esterolytic reaction, and in which R' is an amino acid or peptide moiety having a nitro-gen protective group, and 3-quinuclidinol.
Claim 3
Dependent Claim
12 words
The composition of claim 1 in which R is indoxyl- or thioindoxyl-.
Claim 4
Dependent Claim
10 words
The composition of claim 1 in which R is indoxyl-.
Claim 5
Dependent Claim
13 words
The composition of claim 1 in which R' is an amino acid residue.
Claim 6
Dependent Claim
12 words
The composition of claim 1 in which R' is a peptide residue.
Claim 7
Dependent Claim
11 words
The composition of claim 1 in which R' is N-tosyl alaninate.
Claim 8
Dependent Claim
12 words
The composition of claim 1 in which the chromogenic ester is indoxyl-N-tosylalaninate.
Claim 9
Dependent Claim
20 words
The composition of claim 1, 2 or 3 in which the composition further comprises an alcohol having 1-20 carbon atoms.
Claim 10
Dependent Claim
20 words
The composition of claim 4, 5 or 6 in which the composition further comprises an alcohol having 1-20 carbon atoms.
Claim 11
Dependent Claim
17 words
The composition of claim 7 in which the com-position further comprises an alcohol having 1-20 carbon atoms.
Claim 12
Dependent Claim
15 words
The composition of claim 1, 2 or 3 in which the composition further comprises decanol.
Claim 13
Dependent Claim
15 words
The composition of claim 4, 5 or 6 in which the composition further comprises decanol.
Claim 14
Dependent Claim
12 words
The composition of claim 7 in which the com-position further comprises decanol.
Claim 15
Dependent Claim
31 words
A test device for determining the presence of esterase activity in a liquid test sample, said de-vice comprising a carrier matrix incorporated with the composition of claim 1, 2 or 3.
Claim 16
Dependent Claim
31 words
A test device for determining the presence of esterase activity in a liquid test sample, said de-vice comprising a carrier matrix incorporated with the composition of claim 4, 5 or 6.
Claim 17
Dependent Claim
28 words
A test device for determining the presence of esterase activity in a liquid test sample, said de-vice comprising a carrier matrix incorporated with the composition of claim 7.
Claim 18
Dependent Claim
43 words
A test device for determining the presence of esterase activity in a liquid test sample, said device comprising a carrier matrix incorporated with the composition of claim 1, 2 or 3 in which the com-position further comprises an alcohol having 1-20 carbon atoms.
Claim 19
Dependent Claim
43 words
A test device for determining the presence of esterase activity in a liquid test sample, said device comprising a carrier matrix incorporated with the composition of claim 4, 5 or 6 in which the com-position further comprises an alcohol having 1-20 carbon atoms.
Claim 20
Dependent Claim
40 words
A test device for determining the presence of esterase activity in a liquid test sample, said device comprising a carrier matrix incorporated with the composition of claim 7 in which the composition further comprises an alcohol having 1-20 carbon atoms.
Claim 21
Dependent Claim
38 words
A test device for determining the presence of esterase activity in a liquid test sample, said device comprising a carrier matrix incorporated with the composition of claim 1, 2 or 3 in which the com-position further comprises decanol.
Claim 22
Dependent Claim
38 words
A test device for determining the presence of esterase activity in a liquid test sample, said device comprising a carrier matrix incorporated with the composition of claim 4, 5 or 6 in which the com-position further comprises decanol.
Claim 23
Dependent Claim
35 words
A test device for determining the presence of esterase activity in a liquid test sample, said device comprising a carrier matrix incorporated with the composition of claim 7 in which the composition further comprises decanol.
Claim 24
Dependent Claim
51 words
A method for determining the presence of esterase activity in a liquid test sample, said method comprising the steps of contacting the test sample with a test device comprising a carrier ma-trix incorporated with the composition of claim 1, 2 or 3 and observing a detectable response from the carrier matrix.
Claim 25
Dependent Claim
51 words
A method for determining the presence of esterase activity in a liquid test sample, said method comprising the steps of contacting the test sample with a test device comprising a carrier ma-trix incorporated with the composition of claim 4, 5 or 6 and observing a detectable response from the carrier matrix.
Claim 26
Dependent Claim
48 words
A method for determining the presence of esterase activity in a liquid test sample, said method comprising the steps of contacting the test sample with a test device comprising a carrier ma-trix incorporated with the composition of claim 7 and observing a detectable response from the car-rier matrix.
Claim 27
Dependent Claim
63 words
A method for determining the presence of esterase activity in a liquid test sample, said method comprising the steps of contacting the test sample with a test device comprising a carrier ma-trix incorporated with the composition of claim 1, 2 or 3 in which the composition further comprises an alcohol having 1-20 carbon atoms and observing a detectable response from the carrier matrix.
Claim 28
Dependent Claim
63 words
A method for determining the presence of esterase activity in a liquid test sample, said method comprising the steps of contacting the test sample with a test device comprising a carrier ma-trix incorporated with the composition of claim 4, 5 or 6 in which the composition further comprises an alcohol having 1-20 carbon atoms and observing a detectable response from the carrier matrix.
Claim 29
Dependent Claim
60 words
A method for determining the presence of esterase activity in a liquid test sample, said method comprising the steps of contacting the test sample with a test device comprising a carrier ma-trix incorporated with the composition of claim 7 in which the composition further comprises an al-cohol having 1-20 carbon atoms and observing a de-tectable response from the carrier matrix.
Claim 30
Dependent Claim
58 words
A method for determining the presence of esterase activity in a liquid test sample, said method comprising the steps of contacting the test sample with a test device comprising a carrier ma-trix incorporated with the composition of claim 1, 2 or 3 in which the composition further comprises decanol and observing a detectable response from the carrier matrix.
Claim 31
Dependent Claim
58 words
A method for determining the presence of esterase activity in a liquid test sample, said method comprising the steps of contacting the test sample with a test device comprising a carrier ma-trix incorporated with the composition of claim 4, 5 or 6 in which the composition further comprises decanol and observing a detectable response from the carrier matrix.
Claim 32
Dependent Claim
55 words
A method for determining the presence of esterase activity in a liquid test sample, said method comprising the steps of contacting the test sample with a test device comprising a carrier ma-trix incorporated with the composition of claim 7 in which the composition further comprises decanol and observing a detectable response from the car-rier matrix.
References Cited (Backward Citations)
No citation records indexed.
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