CA1123336A — Method for the demonstration and determination o…

Claim 1

Independent Claim 304 words

A method for the detection and determination of an antigen or anti-body in a liquid sample containing said antigen or antibody which comprises carrying out the following sequence of process steps:-(A) (a) providing a predetermined quantity of a labelled antibody against said antigen; (b) providing a predetermined quantity of a labelled antibody against said antigen, said labelled antibody being derived from a different animal species than said first antibody; (c) providing an insolubilized antibody against said first antibody; (d) reacting the components of steps (a), (b) and (c) with a liquid sample suspected of containing the antigen to form a solid and a liquid phase; and (e) estimating the amount of labelled antibody in either the liquid or solid phase, said amount being an indication of the amount of antigen present in the liquid sample analyzed; or (B) (a) providing a predetermined amount of an antigen against which the first antibody is directed; (b) providing a second antibody also directed against said antigen, said second antibody derived from a different species of animal than said first antibody; (c) providing an insolubilized antibody directed against either the first or second antibody; (d) providing a labelled antibody directed against either the first or second antibody, provided that both the insolubilized and labelled antibodies are not directed against the same antibody; (e) reacting the components of steps (a) through (d) with a liquid sample suspected of containing the first antibody, thereby forming a solid and a liquid phase; and (f) estimating the amount of labelled antibody in either the liquid or solid phases, said amount being an indication of the amount of the first anti-body present in the sample to be analyzed; the selection of sequence (A) or (B) being entirely dependent on whether an antigen or antibody is to be determined in a given liquid sample.

Claim 2

Independent Claim 304 words

A method for the detection and determination of an antigen or anti-body in a liquid sample containing said antigen or antibody which comprises carrying out the following sequence of process steps:-(A) (a) providing a predetermined quantity of a labelled antibody against said antigen; (b) providing a predetermined quantity of a labelled antibody against said antigen, said labelled antibody being derived from a different animal species than said first antibody; (c) providing an insolubilized antibody against said first antibody; (d) reacting the components of steps (a), (b) and (c) with a liquid sample suspected of containing the antigen to form a solid and a liquid phase; and (e) estimating the amount of labelled antibody in either the liquid or solid phase, said amount being an indication of the amount of antigen present in the liquid sample analyzed; or (B) (a) providing a predetermined amount of an antigen against which the first antibody is directed; (b) providing a second antibody also directed against said antigen, said second antibody derived from a different species of animal than said first antibody; (c) providing an insolubilized antibody directed against either the first or second antibody; (d) providing a labelled antibody directed against either the first or second antibody, provided that both the insolubilized and labelled antibodies are not directed against the same antibody; (e) reacting the components of steps (a) through (d) with a liquid sample suspected of containing the first antibody, thereby forming a solid and a liquid phase; and (f) estimating the amount of labelled antibody in either the liquid or solid phases, said amount being an indication of the amount of the first anti-body present in the sample to be analyzed; the selection of sequence (A) or (B) being entirely dependent on whether an antigen or antibody is to be determined in a given liquid sample.

Claim 3

Independent Claim 126 words

A method for the detection and determination of an antigen in a liquid sample containing said antigen comprising the steps of: (a) providing a predetermined quantity of a labelled antibody against said antigen; (b) providing a predetermined quantity of a labelled antibody against said antigen, said labelled antibody being derived from a different animal species than said first antibody; (c) providing an insolubilized antibody against said first antibody; (d) reacting the components of steps (a), (b), and (c) with a liquid sample suspected of containing the antigen to form a solid and a liquid phase; and (e) estimating the amount of labelled antibody in either the liquid or solid phase, said amount being an indication of the amount of antigen present in the liquid sample analyzed.

Claim 4

Independent Claim 126 words

A method for the detection and determination of an antigen in a liquid sample containing said antigen comprising the steps of: (a) providing a predetermined quantity of a labelled antibody against said antigen; (b) providing a predetermined quantity of a labelled antibody against said antigen, said labelled antibody being derived from a different animal species than said first antibody; (c) providing an insolubilized antibody against said first antibody; (d) reacting the components of steps (a), (b), and (c) with a liquid sample suspected of containing the antigen to form a solid and a liquid phase; and (e) estimating the amount of labelled antibody in either the liquid or solid phase, said amount being an indication of the amount of antigen present in the liquid sample analyzed.

Claim 5

Dependent Claim 20 words

A method as in claim 2 wherein the insolubilized antibody is attached to the reaction surface of a polystyrene tube.

Claim 6

Dependent Claim 22 words

A method as in claim 2 wherein the label on the antibody of step (b) is a radioactive isotope or an enzyme.

Claim 7

Dependent Claim 14 words

A method as in claim 2 wherein the antigen is a hepatitis associated antigen.

Claim 8

Independent Claim 157 words

A method for the detection and determination of a first antibody in a liquid sample comprising: (b) providing a predetermined amount of an antigen against which the first antibody is directed; (b) providing a second antibody also directed against said antigen, said second antibody derived from a different species of animal than said first antibody; (c) providing an insolubilized antibody directed against either the first or second antibody; (d) providing a labelled antibody directed against either the first or second antibody, provided that both the insolubilized and labelled anti-bodies are not directed against the same antibody; (e) reacting the components of steps (a) through (d) with a liquid sample suspected of containing the first antibody, thereby forming a solid and a liquid phase; and (f) estimating the amount of labelled antibody in either the liquid or solid phases, said amount being an indication of the amount of the first antibody present in the sample to be analyzed.

Claim 9

Independent Claim 157 words

A method for the detection and determination of a first antibody in a liquid sample comprising: (b) providing a predetermined amount of an antigen against which the first antibody is directed; (b) providing a second antibody also directed against said antigen, said second antibody derived from a different species of animal than said first antibody; (c) providing an insolubilized antibody directed against either the first or second antibody; (d) providing a labelled antibody directed against either the first or second antibody, provided that both the insolubilized and labelled anti-bodies are not directed against the same antibody; (e) reacting the components of steps (a) through (d) with a liquid sample suspected of containing the first antibody, thereby forming a solid and a liquid phase; and (f) estimating the amount of labelled antibody in either the liquid or solid phases, said amount being an indication of the amount of the first antibody present in the sample to be analyzed.

Claim 10

Dependent Claim 29 words

A method as in claim 6 wherein the insolubilized antibody is directed against the second antibody and the labelled antibody is directed against the first antibody to be determined.

Claim 11

Dependent Claim 71 words

A method as in claim 6 for detecting the presence of hepatitis immune complexes in human serum suspected of containing said complexes, said first antibody being directed against hepatitis associated antigen, said antigen being a hepatitis associated antigen, said antigen and first antibody being in the form of an immune complex and wherein the presence of said complex is determined by reaction with the reagents of steps (b), (c) and (d).

Claim 12

Dependent Claim 15 words

A method as in claim 6 wherein the first antibody is directed against rubella virus.

Claim 13

Dependent Claim 190 words

A process for the detection or determination of an at least bivalent antigen in a liquid sample, said process comprising the steps of: (a) providing a given quantity of a first antibody against said at least bivalent antigen to be detected or determined, which first antibody is or has been labelled; (b) providing a given quantity of a second antibody against said at least bivalent antigen to be detected or determined, said second antibody being produced in a different animal species than said first antibody; (c) providing a given quantity of an insolubilized third antibody against said second antibody; (d) contacting and incubating a sample of a fluid containing said at least bivalent antigen to be detected or determined with the components of steps (a), (b) and (c) to form a reaction mixture having a liquid phase and a solid phase; (e) separating the liquid phase from the solid phase; and (f) detecting or determining the labelled component in either the liquid phase or the solid phase, which detection or determination is a measure of the presence or quantity of said at least bivalent antigen to be detected or determined.

Claim 14

Dependent Claim 190 words

A process for the detection or determination of an at least bivalent antigen in a liquid sample, said process comprising the steps of: (a) providing a given quantity of a first antibody against said at least bivalent antigen to be detected or determined, which first antibody is or has been labelled; (b) providing a given quantity of a second antibody against said at least bivalent antigen to be detected or determined, said second antibody being produced in a different animal species than said first antibody; (c) providing a given quantity of an insolubilized third antibody against said second antibody; (d) contacting and incubating a sample of a fluid containing said at least bivalent antigen to be detected or determined with the components of steps (a), (b) and (c) to form a reaction mixture having a liquid phase and a solid phase; (e) separating the liquid phase from the solid phase; and (f) detecting or determining the labelled component in either the liquid phase or the solid phase, which detection or determination is a measure of the presence or quantity of said at least bivalent antigen to be detected or determined.

Claim 15

Dependent Claim 21 words

The process of claim 10 wherein the first antibody is labelled by adding a labelled fourth antibody against said first antibody.

Claim 16

Dependent Claim 21 words

The process of claim 10 wherein the insolubilized third antibody is attached to the reaction surface of a polystyrene test tube.

Claim 17

Dependent Claim 13 words

The process of claim 10 wherein the antigen is a hepatitis associated antigen.

Claim 18

Dependent Claim 17 words

The process of claim 10 or 11 wherein the label is a radioactive isotope or an enzyme.

Claim 19

Dependent Claim 184 words

A process for the detection or determination of an antibody in a liquid sample, said process comprising the steps of: (a) providing a given quantity of an antigen against which an antibody to be detected or determined is directed; (b) providing a given quantity of a second antibody against said anti-gen, said second antibody being produced in a different animal species than said antibody to be detected or determined, thereby either said antibody to be detected or determined or said second antibody is or has been labelled; (c) providing a given quantity of an insolubilized third antibody against the non-labelled antibody; (d) contacting and incubating the components of steps (a), (b) and (c) with a liquid sample containing said antibody to be detected or determined to form a reaction mixture having a solid phase and a liquid phase; (e) separating the solid phase from the liquid phase; and (f) detecting or determining the labelled component in either the liquid phase or the solid phase, which detection or determination is a measure of the presence or quantity of said antibody to be detected or deter-mined.

Claim 20

Dependent Claim 184 words

A process for the detection or determination of an antibody in a liquid sample, said process comprising the steps of: (a) providing a given quantity of an antigen against which an antibody to be detected or determined is directed; (b) providing a given quantity of a second antibody against said anti-gen, said second antibody being produced in a different animal species than said antibody to be detected or determined, thereby either said antibody to be detected or determined or said second antibody is or has been labelled; (c) providing a given quantity of an insolubilized third antibody against the non-labelled antibody; (d) contacting and incubating the components of steps (a), (b) and (c) with a liquid sample containing said antibody to be detected or determined to form a reaction mixture having a solid phase and a liquid phase; (e) separating the solid phase from the liquid phase; and (f) detecting or determining the labelled component in either the liquid phase or the solid phase, which detection or determination is a measure of the presence or quantity of said antibody to be detected or deter-mined.

Claim 21

Dependent Claim 28 words

The process of claim 15 wherein either the first antibody or the second antibody is labelled by adding a labelled fourth antibody against said first or second antibody.

Claim 22

Dependent Claim 16 words

The process of claim 15 or 16 wherein the label is a radioactive isotope or enzyme.

Claim 23

Dependent Claim 14 words

The process of claim 15 wherein the first antibody is directed against rubella virus.

Claim 24

Dependent Claim 256 words

A process for the detection or determination of an at least bivalent antigen in a liquid sample, said process comprising the steps of: (a) providing a given quantity of a first antibody against said at least bivalent antigen to be detected or determined, which first antibody is or has been labelled, said given quantity being at least immunochemically equivalent to the maximum amount of said at least bivalent antigen to be detected or determined; (b) providing a given quantity of a second antibody against said at least bivalent antigen to be detected or determined, said second antibody being produced in a different animal species than said first antibody, said given quantity being at least immunochemically equivalent to the maximum amount of said at least bivalent antigen to be detected or determined; (c) providing a given quantity of an insolubilized third antibody against said second antibody, said given quantity being at least immuno-chemically equivalent to the maximum amount of said second antibody provided in step (b); (d) contacting and incubating a sample of a fluid containing said at least bivalent antigen to be detected or determined with the components of steps (a), (b) and (c) to form a reaction mixture having a liquid phase and a solid phase; (e) separating the liquid phase from the solid phase; and (f) detecting or determining the labelled component in either the liquid phase or the solid phase, which detection or determination is a measure of the presence or quantity of said at least bivalent antigen to be detected or determined.

Claim 25

Dependent Claim 256 words

A process for the detection or determination of an at least bivalent antigen in a liquid sample, said process comprising the steps of: (a) providing a given quantity of a first antibody against said at least bivalent antigen to be detected or determined, which first antibody is or has been labelled, said given quantity being at least immunochemically equivalent to the maximum amount of said at least bivalent antigen to be detected or determined; (b) providing a given quantity of a second antibody against said at least bivalent antigen to be detected or determined, said second antibody being produced in a different animal species than said first antibody, said given quantity being at least immunochemically equivalent to the maximum amount of said at least bivalent antigen to be detected or determined; (c) providing a given quantity of an insolubilized third antibody against said second antibody, said given quantity being at least immuno-chemically equivalent to the maximum amount of said second antibody provided in step (b); (d) contacting and incubating a sample of a fluid containing said at least bivalent antigen to be detected or determined with the components of steps (a), (b) and (c) to form a reaction mixture having a liquid phase and a solid phase; (e) separating the liquid phase from the solid phase; and (f) detecting or determining the labelled component in either the liquid phase or the solid phase, which detection or determination is a measure of the presence or quantity of said at least bivalent antigen to be detected or determined.

Claim 26

Dependent Claim 16 words

The process of claim 19, wherein said insolubilized third antibody of step (c) is in suspension.

Claim 27

Dependent Claim 16 words

The process of claim 20, wherein said insolubilized third anti-body is in a suspension of cellulose.

Claim 28

Dependent Claim 13 words

The process of claim 20, wherein said at least bivalent antigen is HBAg.

Claim 29

Dependent Claim 22 words

The process of claim 20 wherein the given quantities of said first antibody (a) and said second antibody (b) are immunochemically equivalent.

Claim 30

Dependent Claim 14 words

The process of claim 19, wherein said first antibody is labelled with horseradish peroxidase.

Claim 31

Dependent Claim 15 words

The process of claim 19, wherein said at least bivalent antigen is human chorionic gonadotrophin.

Claim 32

Dependent Claim 62 words

The process of claim 19, further comprising the steps of: first, contacting and incubating components (a) and (b) with the liquid sample containing said at least bivalent antigen to be detected or determined to form an intermediate reaction mixture; and contacting and incubating said intermediate reaction mixture with the insolubilized third antibody of step (c) in the manner recited in step (d).

Claim 33

Dependent Claim 93 words

The process of claim 19, further comprising the steps of: first, contacting and incubating the second antibody provided in step (b) with the liquid sample containing said at least bivalent antigen to be detected or determined to form a first intermediate reaction mixture; second, contacting and incubating said first intermediate reaction mix-ture with the labelled antibody provided in step (a) to form a second inter-mediate reaction mixture; and third, contacting and incubating said second intermediate reaction mixture with the insolubilized third antibody provided in step (c) in the manner recited in step (d).

Claim 34

Dependent Claim 95 words

The process of claim 19, further comprising the steps of: first, contacting and incubating the insolubilized third antibody of step (c) with the liquid sample containing said at least bivalent antigen to be detected or determined and said second antibody of step (b) to form an intermediate reaction mixture having an intermediate solid phase and an intermediate liquid phase; second, separating the intermediate solid phase from the intermediate liquid phase; and third, contacting and incubating the intermediate solid phase with the labelled first antibody provided in step (a) in the manner recited in step (d).

Claim 35

Dependent Claim 21 words

The process of any of claims 26, 27 or 28, wherein said at least bivalent antigen is hepatitis B surface antigen.

Claim 36

Dependent Claim 20 words

The process of any of claims 26, 27 or 28, wherein said at least bivalent antigen is human chorionic gonadotrophin.

Claim 37

Dependent Claim 23 words

The process of any of claims 26, 27 or 28, wherein said at least bivalent antigen is the third component in human serum.

Claim 38

Dependent Claim 23 words

The process of any of claims 26, 27 or 28, wherein said first anti-body provided in step (a) is labelled with an enzyme.

Claim 39

Dependent Claim 240 words

A process for the detection or determination of an antibody in a liquid sample, said process comprising the steps of: (a) providing a given quantity of an antigen against which an antibody to be detected or determined is directed, said given quantity being at least immunochemically equivalent to the maximum amount of antibody to be detected or determined; (b) providing a given quantity of a second antibody against said antigen, said second antibody being produced in a different animal species than said antibody to be detected or determined, whereby said second anti-body is or has been labelled, said given quantity of labelled second antibody being at least immunochemically equivalent to the maximum amount of antigen provided in step (a); (c) providing a given quantity of an insolubilized third antibody against the antibody to be detected or determined, said given quantity being at least immunochemically equivalent to the maximum amount of said antibody to be detected or determined; (d) contacting and incubating the components of steps (a), (b) and (c) with a liquid sample containing said antibody to be detected or determined to form a reaction mixture having a solid phase and a liquid phase; (e) separating the solid phase from the liquid phase; and (f) detecting or determining the labelled component in either the liquid phase or the solid phase, which detection or determination is a mea-sure of the presence or quantity of said antibody to be detected or determined.

Claim 40

Dependent Claim 240 words

A process for the detection or determination of an antibody in a liquid sample, said process comprising the steps of: (a) providing a given quantity of an antigen against which an antibody to be detected or determined is directed, said given quantity being at least immunochemically equivalent to the maximum amount of antibody to be detected or determined; (b) providing a given quantity of a second antibody against said antigen, said second antibody being produced in a different animal species than said antibody to be detected or determined, whereby said second anti-body is or has been labelled, said given quantity of labelled second antibody being at least immunochemically equivalent to the maximum amount of antigen provided in step (a); (c) providing a given quantity of an insolubilized third antibody against the antibody to be detected or determined, said given quantity being at least immunochemically equivalent to the maximum amount of said antibody to be detected or determined; (d) contacting and incubating the components of steps (a), (b) and (c) with a liquid sample containing said antibody to be detected or determined to form a reaction mixture having a solid phase and a liquid phase; (e) separating the solid phase from the liquid phase; and (f) detecting or determining the labelled component in either the liquid phase or the solid phase, which detection or determination is a mea-sure of the presence or quantity of said antibody to be detected or determined.

Claim 41

Dependent Claim 14 words

The process of claim 33, wherein the third antibody is adsorbed to a tube.

Claim 42

Dependent Claim 12 words

The process of claim 34, wherein said tube is a polystyrene tube.

Claim 43

Dependent Claim 19 words

The process of claim 33, wherein said antibody to be detected or determined is an antibody against rubella virus.

Claim 44

Dependent Claim 14 words

The process of claim 33, wherein said second antibody is labelled with an enzyme.

Claim 45

Dependent Claim 11 words

The process of claim 37, wherein said enzyme is horseradish peroxidase.

Claim 46

Dependent Claim 74 words

The process of claim 33, further comprising the steps of: first, contacting and incubating components (a) and (c) with the liquid sample containing the antibody to be detected or determined to form an intermediate solid phase and an intermediate liquid phase; separating the intermediate solid phase and the intermediate liquid phase; and contacting and incubating said intermediate solid phase with the labelled second antibody of step (b) in the manner recited in step (d).

Claim 47

Dependent Claim 131 words

A test system for the determination of an antigen or antibody by the method of claim 1, comprising: (a) either a predetermined quantity of a first antibody against an antigen to be determined or a predetermined amount of an antigen against which a first antibody to be determined is directed; (b) a predetermined quantity of a second antibody also directed against said antigen, said second antibody being derived from a different animal species than said first antibody; and (c) a predetermined amount of an insolubilized antibody directed against either the first or second antibodies; and (d) where an antibody is to be detected and/or determined, a labelled antibody directed against either the first or second antibodies, provided that both the insolubilized and labelled antibodies are not directed against the same antibody.

Claim 48

Dependent Claim 68 words

A test system for the determination of an antigen by the method of claim 2, comprising: (a) a predetermined quantity of a first antibody against the antigen to be determined; (b) a predetermined quantity of a labelled antibody against said antigen, said labelled antibody being derived from a different animal species than said first antibody; and (c) a predetermined amount of an insolubilized antibody against said first antibody.

Claim 49

Dependent Claim 108 words

A test system for the detection and determination of a first antibody in a liquid sample by the method of claim 6, comprising: (a) a predetermined amount of an antigen against which the first antibody is directed; (b) a predetermined amount of a second antibody also directed against said antigen, said second antibody derived from a different species of animal than said first antibody; (c) a predetermined amount of an insolubilized antibody directed against either the first or second antibodies; and (d) a labelled antibody directed against either the first or second antibodies, provided that both the insolubilized and labelled antibodies are not directed against the same antibody.

Claim 50

Dependent Claim 27 words

A test system as in claim 42 wherein the insolubilized antibody is directed against the second antibody and the labelled antibody is directed against the first antibody.

Claim 51

Dependent Claim 80 words

A test pack for the detection or determination of an at least bivalent antigen in a fluid sample comprising: (a) a given amount of a first antibody against the at least bivalent antigen to be detected or determined; (b) a given amount of a labelled second antibody against said at least bivalent antigen, said second antibody being produced in a different animal species than the first antibody; (c) a given amount of an insolubilized third antibody against said first antibody.

Claim 52

Dependent Claim 80 words

A test pack for the detection or determination of an at least bivalent antigen in a fluid sample comprising: (a) a given amount of a first antibody against the at least bivalent antigen to be detected or determined; (b) a given amount of a labelled second antibody against said at least bivalent antigen, said second antibody being produced in a different animal species than the first antibody; (c) a given amount of an insolubilized third antibody against said first antibody.

Claim 53

Dependent Claim 92 words

A test pack for the detection or determination of an at least bivalent antigen in a fluid sample comprising: (a) a given amount of a first antibody against the at least bivalent antigen to be detected or determined; (b) a given amount of a second antibody against said at least bivalent antigen, said second antibody being produced in a different animal species than the first antibody; (c) a given amount of a labelled third antibody against said second antibody; (d) a given amount of an insolubilized fourth antibody against said first antibody.

Claim 54

Dependent Claim 92 words

A test pack for the detection or determination of an at least bivalent antigen in a fluid sample comprising: (a) a given amount of a first antibody against the at least bivalent antigen to be detected or determined; (b) a given amount of a second antibody against said at least bivalent antigen, said second antibody being produced in a different animal species than the first antibody; (c) a given amount of a labelled third antibody against said second antibody; (d) a given amount of an insolubilized fourth antibody against said first antibody.

Claim 55

Dependent Claim 78 words

A test pack for the detection or determination of an antibody in a fluid sample comprising: (a) a given amount of an antigen for which the antibody to be detected or determined possesses affinity; (b) a given amount of a labelled second antibody, said second antibody being produced in a different animal species than the antibody to be detected or determined; (c) a given amount of an insolubilized third antibody against the antibody to be detected or determined.

Claim 56

Dependent Claim 78 words

A test pack for the detection or determination of an antibody in a fluid sample comprising: (a) a given amount of an antigen for which the antibody to be detected or determined possesses affinity; (b) a given amount of a labelled second antibody, said second antibody being produced in a different animal species than the antibody to be detected or determined; (c) a given amount of an insolubilized third antibody against the antibody to be detected or determined.

Claim 57

Dependent Claim 108 words

A test pack for the detection or determination of an antibody in a fluid sample comprising: (a) a given amount of an antigen for which the antibody to be detected or determined possesses affinity; (b) a given amount of a second antibody, said second antibody being produced in a different animal species than the antibody to be detected or determined; (c) a given amount of a labelled third antibody against either the antibody to be detected or determined or the second antibody; (d) a given amount of an insolubilized fourth antibody directed against the other of the two antibodies to which the labelled third antibody (c) is directed.

Claim 58

Dependent Claim 108 words

A test pack for the detection or determination of an antibody in a fluid sample comprising: (a) a given amount of an antigen for which the antibody to be detected or determined possesses affinity; (b) a given amount of a second antibody, said second antibody being produced in a different animal species than the antibody to be detected or determined; (c) a given amount of a labelled third antibody against either the antibody to be detected or determined or the second antibody; (d) a given amount of an insolubilized fourth antibody directed against the other of the two antibodies to which the labelled third antibody (c) is directed.

Claim 59

Dependent Claim 163 words

A test pack for the detection or determination of an at least bivalent antigen to be detected or determined in a liquid sample according to the process of claim 19 comprising: (a) a given quantity of a first antibody against the at least bivalent antigen to be detected or determined, said given quantity being at least immunochemically equivalent to the maximum amount of said at least bivalent antigen to be detected or determined; (b) a given quantity of a second antibody against said at least bivalent antigen to be detected or determined, said second antibody being produced in a different animal species than said first antibody, said given quantity of second antibody being at least immunochemically equivalent to the maximum amount of said at least bivalent antigen to be detected or determined; and (c) a given quantity of an insolubilized third antibody against said second antibody, said given quantity being at least immunochemically equivalent to the maximum amount of second antibody in (b).

Claim 60

Dependent Claim 162 words

A test pack for the detection or determination of an antibody in a liquid sample according to the process of claims 33 or 39 comprising: (a) a given quantity of an antigen against which an antibody to be detected or determined is directed, said given quantity being at least immunochemically equivalent to the maximum amount of said antibody to be detected or determined; (b) a given quantity of a second antibody against said antigen, said second antibody being produced in a different animal species than said antibody to be detected or determined, whereby said second antibody is or has been labelled and said given quantity of second labelled antibody being at least immunochemically equivalent to the maximum of said antigen in (a); and (c) a given quantity of insolubilized third antibody against the antibody to be detected or determined, said given quantity of insolubilized antibody being at least immunochemically equivalent to the maximum amount of said antibody to be detected or determined.

Claim 61

Dependent Claim 21 words

The test pack of claim 48, where the given quantities of said first antibody and said second antibody are immunochemically equivalent.

Method for the demonstration and determination of an antigen or antibody

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